Pursuit of a new system for a high throughput screening of drugs to develop a new strategy of prevention and treatment of ATL
Project/Area Number |
20390267
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Hematology
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Research Institution | The University of Tokyo |
Principal Investigator |
WATANABE Toshiki The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (30182934)
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Co-Investigator(Kenkyū-buntansha) |
TSUKASAKI Kunihiro 長崎大学, 医歯薬学総合研究科, 准教授 (40274659)
ISHIDA Takaomi 東京大学, 医科学研究所, 特任准教授 (80293447)
NAKANO Kazumi 東京大学, 大学院・新領域創成科学研究科, 助教 (60549591)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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Keywords | ATL / 発現解析 / 薬効評価 / トキシコゲノミクス / ハイスループット / イマチニブ / セファランチン / miRNA |
Research Abstract |
We prepared a gene panel of 12 genes that show abnormal expression levels on ATL cells based on the expression profile analyses. The expression levels of these genes were measured by QuantiGene Plex simultaneously and in a short time. The measured values were transformed into an "ATL score" by calculation base on a formula. "ATL score" was used to monitor effects of drugs against ATL cells and cell lines derived from them, although experiments using this system were limited because of the cost. In the process of establishing this system, we obtained detailed information about the expression levels of specific genes in PBMCs of HTLV-1 carriers and ATL patients, which provided information about the changes in expression levels in the course of transformation of HTLV-1-infected cells. Conventional analyses were done on some chemicals as to the effects on the ATL cell lines, which provided interesting information about the ATL-specific activities of these molecules.
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Report
(4 results)
Research Products
(92 results)
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[Journal Article] Intra-and inter-laboratory Variability in HTLV-1 Proviral Load Quantification Using Real-Time Polymerase Chain Reaction Assays : A Multi-Center Study.2010
Author(s)
Kamihira S, Iwanaga M, Sasaki D, Yamano Y, Okayama A, Umeki K, Kubota R, Izumo S, Yamaguchi K, Watanabe T, Satake M.
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Journal Title
Cancer Sci 101(11)
Pages: 2361-2367
Related Report
Peer Reviewed
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[Journal Article] Long-term study of indolent adult T-cell leukemia-lymphoma (ATL).2010
Author(s)
Takasaki Y, Iwanaga M, Imaizumi Y, Tawara M, Joh T, Kohno T, Yamada Y, Kamihira S, Ikeda S, Miyazaki Y, Tomonaga M, Tsukasaki K.
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Journal Title
Blood 115
Pages: 4337-4343
Related Report
Peer Reviewed
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[Journal Article] Intra-and inter-laboratory variability in HTLV-1 proviral load quantification using real-time polymerase chain reaction assays : A Multi-Center Study2010
Author(s)
Kamihira S, Iwanaga M, Sasaki D, Yamano Y, Okayama A, Umeki K, Kuboto R, Izumo S, Yamaguchi K, Watanabe T, Satake M
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Journal Title
Cancer Science
Volume: 101
Pages: 2361-2367
Related Report
Peer Reviewed
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[Journal Article] CADM1 interacts with Tiaml and promotes invasive phenotype of human T-cell leukemia virus type I-transformed cells and adult T-cell leukemia cells2010
Author(s)
Masuda M, Ohta T, Maruyama T, Ito A, Hayashi T, Tsukasaki K, Kamihira S, Yamaoka S, Hoshino H, Yoshida T, Watanabe T
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Journal Title
J Biol Chem
Volume: 285(20)
Pages: 15511-15522
Related Report
Peer Reviewed
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[Journal Article] CADM1 INTERACTS WITH TIAM1 AND PROMOTES INVASIVE PHENOTYPE OF HU MAN T-CELL LEUKEMIA VIRUS TYPE I(HTLV-I)TRANSFORMED CELLS AND ADULT T-CELL LEUKEMIA(ATL)CELLS : POSSIBLE INVOLVEMENT OF CADM1 IN PATHOGENESIS OF ATL2010
Author(s)
Masuda M, Ohta T, Maruyama T, Ito A, Hayashi T, Tsukasaki K, Kamihira S, Yamaoka S, Hoshino H, Yoshida T, Watanabe T, Stanbridge E-J, Murakam Y
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Journal Title
J Biol Chem (in press, 掲載確定)
Related Report
Peer Reviewed
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[Journal Article] Long-term study of indolent adult T-cell leukemia-lymphoma(ATL)2010
Author(s)
Takasaki Y, Iwanaga M, Imaizumi Y, Tawara M, Joh T, Kohno T, Yamada Y, Kamihira S, Ikeda S, Miyazaki Y, Tomonaga M, Tsukasaki K
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Activation of p53 by Nutlin-3a, an antagonist of MDM2, induces apoptosis and cellular senescence in adult T-cell leukemia cells.2009
Author(s)
Hasegawa H, Yamada Y, Iha H, Tsukasaki K, Nagai K, Atogami S, Sugahara K, Tsuruda K, Ishizaki A, Kamihira S.
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Journal Title
Leukemia 23
Pages: 2090-2101
NAID
Related Report
Peer Reviewed
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[Journal Article] Definition, prognostic factors, treatment and response criteria of adult T-cell leukemia-lymphoma : a proposal from an international consensus meeting.2009
Author(s)
Tsukasaki K, Hermine O, Bazarbachi A, Ratner L, Ramos CJ, Harrington Jr W, O'Mahony D, Janik J, Bittencourt AL, Taylor GP, Yamaguchi K, Utsunomiya A, Tobinai K, Watanabe T.
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Journal Title
J Clin Oncol 27
Pages: 453-459
Related Report
Peer Reviewed
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[Journal Article] Activation of p53 by Nutlin-3a, an antagonist of MDM2, induces apoptosis and cellular senescence in adult T-cell leukemia cells2009
Author(s)
Hasegawa H, Yamada Y, Iha H, Tsukasaki K, Nagai K, Atogami S, Sugahara K, Tsuruda K, Ishizaki A, Kamihira S
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Journal Title
Leukemia 23
Pages: 2090-2101
NAID
Related Report
Peer Reviewed
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[Journal Article]2009
Author(s)
塚崎邦弘
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Journal Title
III悪性リンパ腫-治療の実際-1.病型別治療方針-標準的治療、研究的治療E.成人T細胞白血病/リンパ腫(ATL).(飛内賢正、堀田知光、木下朝博編集、悪性リンパ腫治療マニュアル改訂第3版(南江堂)
Pages: 188-193
Related Report
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[Journal Article] Autocrine and/or paracrine growth of aggressive ATLL cells caused by HGF and c-Met.2008
Author(s)
Onimaru Y, Tsukasaki K, Murata K, Imaizumi Y, Choi YL, Hasegawa H, Sugahara K, Yamada Y, Hayashi T, Nakashima M, Taguchi T, Mano H, Kamihira S, Tomonaga M.
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Journal Title
Int J Oncol 33
Pages: 697-703
NAID
Related Report
Peer Reviewed
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[Journal Article] Adhesion-dependent growth of primary adult T cell leukemia cells with down-regulation of HTLV-I p40Tax protein : a novel in vitro model of the growth of acute ATL cells.2008
Author(s)
Nagai K, Jinnai I, Hata T, Usui T, Sasaki D, Tsukasaki K, Sugahara K, Hishikawa Y, Yamada Y, Tanaka Y, Koji T, Mano H, Kamihira S, Tomonaga M.
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Journal Title
Int J Hematol 88
Pages: 551-564
NAID
Related Report
Peer Reviewed
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[Presentation] Loss of miR-31 links NF-κB activation in Adult T-cell leukemia Viruses, Genes ad Cancer-20102010
Author(s)
Yamagishi M, Nakano K, Miyake A, Kagami Y, Tsutsumi A, Utsunomiya A, Yamaguchi K, Uchimaru K, Watanabe T
Organizer
Viruses, Genes and Cancer-2010
Place of Presentation
Venezia, Italy
Year and Date
2010-09-30
Related Report
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[Presentation] Chronic / smoldering ATL : long-term follow-up and prognostic factor analysis2010
Author(s)
Takasaki Y, Iwanaga M, Imaizumi Y, Tawara M, Joh T, Kohno T, Yamada Y, Kamihira S, Ikeda S, Miyazaki Y, Tomonaga M, Tsukasaki K
Organizer
第72回日本血液学会学術集会
Place of Presentation
横浜
Year and Date
2010-09-26
Related Report
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[Presentation] Molecular pathogenesis of ATL2010
Author(s)
渡邉俊樹
Organizer
The Japanese Society of Hematology/EHA Joint Symposium (Hematology-in-Focus session), The 15th Congress of the European Hematology Association (EHA)
Place of Presentation
Barcelona, Spain(Invited talk)
Year and Date
2010-06-12
Related Report
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[Presentation] Loss of miR-31 links NF-.B activation in Adult T-cell leukemia2010
Author(s)
山岸誠, 中野和民, 三宅在子, 加賀美弥生, 包明久, 宇都宮與, 山口一成, 浅沼里実, 渡邉俊樹
Organizer
Viruses, Genes and Cancer-2010
Place of Presentation
Venezia, Italy
Related Report
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[Presentation] JSPFAD investigators HTLV-1 proviral load and the relation to disease progression in carriers : a nationwide cohort study2010
Author(s)
Iwanaga M, Watanabe T, Utsunomiya A, Okayama A, Uchimaru K, Koh K-R, Ogata M, Kikuchi H, Sagara Y, Uozumi K, Mochizuki M, Tsukasaki K, Saburi Y, Yamamura M, Tanaka J, Moriuchi Y, Hino S, Kamihira S, Yamaguchi K
Organizer
第72回日本血液学会学術集会
Place of Presentation
横浜
Related Report
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[Presentation] Chronic/smoldering ATL: long-term follow-up and prognostic factor analysis2010
Author(s)
Takasaki Y, Iwanaga M, Imaizumi Y, Tawara M, Joh T, Kohno T, Yamada Y, Kamihira S, Ikeda S, Miyazaki Y, Tomonaga M, Tsukasaki K
Organizer
第72回日本血液学会学術集会
Place of Presentation
横浜
Related Report
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[Presentation] Sequential Monitoring of HTLV-1 Provirus Load and Clinical Data in HTLV-1 Infected Individuals in the Joint Study on Predisposing Factors of ATL Development (JSPFAD)2009
Author(s)
Iwanaga M, Watanabe T, Utsunomiya A, Uozumi k, Ogata M, Uchimaru K, Okayama A, Koh K-R, Kamihira S, Yamaguchi K
Place of Presentation
Salvador, Brazil
Year and Date
2009-07-04
Related Report
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[Presentation] Sequential Monitoring of HTLV-1 Provirus Load and Clinical Data in HTLV-1 Infected Individuals in the Joint Study on Predisposing Factors of ATL Development(JSPFAD)2009
Author(s)
Iwanaga M, Watanabe T, Utsunomiya A, Uozumi k, Ogata M, Uchimaru K, Oka yama A, Koh K-R, Kamihira S, Yamaguchi K
Organizer
14th International Conference of Human Retroviroloay : HTLV and related retroviruses
Place of Presentation
Salvador, Brazil
Year and Date
2009-07-04
Related Report
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[Presentation] Possible dysfunction of NMD in ATL cells2009
Author(s)
Takahasi R, Ando T, Nakano K, Utsuno miva A, Yamaguchi K, Watanabe T
Organizer
14th International Conference of Human Retrovirology : HTLV and related retroviruses
Place of Presentation
Salvador, Brazil
Year and Date
2009-07-02
Related Report
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