Construction of the system for hematopoietic cell production from human ES cells and the analysis of its molecular basis toward the development of ES cell therapies

• Project/Area Number: 20390273
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyushu University
• Principal Investigator: TANI Kenzaburo Kyushu University, 生体防御医学研究所, 教授 (00183864)
• Co-Investigator(Kenkyū-buntansha): SUGIYAMA Daisuke 九州大学, 医学研究院, 特任准教授 (00426652)
• Co-Investigator(Renkei-kenkyūsha): KURITA Ryo 理化学研究所, バイオリソースセンター, 研究員 (90380526) ; YOKOO Tomoko 九州大学, 生体防御医学研究所, 研究員 (70596840)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: 血液内科学 / 霊長類ES細胞 / Tal1/Scl遺伝子 / レンチウイルスベクター / 造血細胞分化誘導法 / CD34陽性細胞 / 染色体非挿入型ウイルス / 麻疹ウイルス / 幹細胞 / ヒト胚性幹細胞 / コモンマーモセット / Tall / Scl遺伝子 / NOGマウス / GFP遺伝子 / 麻疹ウイルスベクター / VSV-Gシュードタイプレンチウイルスベクター / ヒト胎児肝臓cDNA発現レンチウイルスベクターラブラリー / コモンマーモセットES細胞 / Tal1 / ヒト胎児肝臓 / LVベクターライブラリー

Research Abstract

In this study, we analyzed the mechanism for hematopoietic cell differentiation by Tal1/Scl gene-transduction for the purpose of developing new therapies using human embryonic stem(ES) cells. We established the ES cell line expressing Tal1/Scl gene constitutively by lentiviral gene transfer and demonstrated that these cell lines produced various types of hematopoietic progenitor cell colonies. And we screened the candidate genes which promote the hematopoiesis as the collaboration with Tal1/Scl gene using high-performance human fetal liver-derived lentiviral cDNA library. We have cloned twenty-nine candidate genes and are now studying their roles in hematopoiesis precisely. These results would give us important information toward the clinical application of human ES cells in future.

Research Products

• [Journal Article] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cells in mice and humans. (2011)
  • Author(s): Inoue T, Sugiyama D, Kurita R, Oikawa T, Kulkeaw K, Kawano H, Miura Y, Okada M, Suehiro Y, Takahashi A, Marumoto T, Inoue H, Komatsu N, Tani K.
  • Journal Title: Stem Cell Rev Rep 7 Pages: 43-52
  • Peer Reviewed
• [Journal Article] Variation in mesodermal and hematopoietic potential of adultskin-derived induced pluripotent stem cell lines in mice. (2011)
  • Author(s): Inoue T, Kulkeaw, K., Okayama, S., Tani, K., Sugiyama, D
  • Journal Title: Stem Cell Rev Rep in press
  • Peer Reviewed
• [Journal Article] Telomerase inhibition promotes an initial step of cell differentiation of primate embryonic stem cell. (2011)
  • Author(s): Maeda,T., Kurita, R., Yokoo, T, Tani, K., Makino, N.
  • Journal Title: Biochem Biophys Res Commun in press
• [Journal Article] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cells in mice and humans. (2011)
  • Author(s): Inoue T, et al
  • Journal Title: Stem Cell Rev Rep Volume: 7 Pages: 43-52
  • Peer Reviewed
• [Journal Article] Variation in mesodermal and hematopoietic potential of adult skin-derived induced pluripotent stem cell lines in mice (2011)
  • Author(s): Inoue T, et al
  • Journal Title: Stem Cell Rev Rep Volume: (In press)
  • Peer Reviewed
• [Journal Article] Leukemic T cells are specifically enriched in a unique CD3(dim) CD7(low) subpopulation of CD4(+) T cells in acute-type adult T-cell leukemia. (2011)
  • Author(s): Tian Y, et al
  • Journal Title: Cancer Sci Volume: 102 Pages: 569-577
  • Peer Reviewed
• [Journal Article] Gene therapy for oral squamous cell carcinoma with IAI.3B promoter-driven oncolytic adenovirus-infected carrier cells. (2011)
  • Author(s): Zhang T, et al
  • Journal Title: Oncol Rep Volume: 25 Pages: 795-802
  • Peer Reviewed
• [Journal Article] A novel potent tumour promoter aberrantly overexpressed in most human cancers. (2011)
  • Author(s): Takahashi, A, et al
  • Journal Title: Scientific Reports Volume: (In press)
  • Peer Reviewed
• [Journal Article] cells are specifically enriched in a unique CD3^<dim>CD7^<low> subpopulation of CD4^+ T cells in acute-type adult T cell leukemia. (2010)
  • Author(s): Tian, Y., Kobayashi, S., Ohno, N., Isobe, M., Tsuda, M., Zaike, Y., Watanabe, N., Tani, K.,Tojo, A., Uchimaru, K.Leukemic T
  • Journal Title: Cancer Sci 14
  • Peer Reviewed
• [Journal Article] CD30 ligand is a target for a novel biological therapy against colitis associated with Th17 responses./CD30 plays a critical role in Th17 differentiation in mice. (2010)
  • Author(s): Sun, X., Yamada, H., Shibata, K., Muta, H., Tani, K., Podack, ER., Iwakura, Y., Yoshikai, Y.
  • Journal Title: J Immunol 185 Pages: 7671-7680
  • Peer Reviewed
• [Journal Article] Enhanced Antitumor Effects of an Oncolytic Measles Virus Vaccine Strain Expressing the Wild-type N, P, L Genes on Human Renal Cell Carcinoma (2010)
  • Author(s): Meng, X., Nakamura, T., Okazaki, T., Inoue, H., Takahashi, A., Miyamoto, S., Sakaguchi, G., Eto, M., Naito, S., Takeda, M., Yanagi, Y., Tani, K.
  • Journal Title: Mol Ther. 18(3) Pages: 544-51
  • Peer Reviewed
• [Journal Article] Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinomaantigen 1 promoter-driven oncolytic adenovirus. (2010)
  • Author(s): Hamada, K., Zhang, T., Desaki, J., Nakashiro, K-I., Ito, H., Tani, K., Koyama, Y.Hamakawa, H.
  • Journal Title: J.Gene Med. 12 Pages: 545-554
  • Peer Reviewed
• [Journal Article] CD30 ligand/CD30 plays a critical role in Th17 differentiation in mice. (2010)
  • Author(s): Sun, X., Yamada, H., Shibata, K., Muta, H., Tani, K., Podack, ER., Yoshikai, Y.
  • Journal Title: J Immunol 185 Pages: 2222-2230
• [Journal Article] Enhanced Antitumor Effects of an Oncolytic Measles Virus Vaccine Strain Expressing the Wild-type N, P, L Genes on Human Renal Cell Carcinoma (2010)
  • Author(s): Meng X, et al
  • Journal Title: Mol Ther Volume: 18 Pages: 544-551
  • Peer Reviewed
• [Journal Article] Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinoma antigen 1 promoter-driven oncolytic adenovirus. (2010)
  • Author(s): Hamada K, et al
  • Journal Title: J Gene Med Volume: 12 Pages: 545-554
  • Peer Reviewed
• [Journal Article] CD30 Ligand/CD30 Plays a Critical Role in Th17 Differentiation in Mice (2010)
  • Author(s): Sun X, et al
  • Journal Title: J Immunol Volume: 185 Pages: 2222-2230
  • Peer Reviewed
• [Journal Article] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cells in mice and humans (2010)
  • Author(s): Inoue T., Sugiyama D., Tani. K., et al.,
  • Journal Title: Stem Cell Rev Rep (in press)
  • Peer Reviewed
• [Journal Article] Tumor growth suppression by adenovirus-mediated introduction of a cell growth suppressing gene tob in a pancreatic cancer model. (2009)
  • Author(s): Yanagie, H., Tanabe, T., Sumimoto, H., Sugiyama, H., Matsuda, S., Nonaka, Y., Ogiwara, N., Sasaki, K., Tani, K.,Takamoto, S., Tkahashi, H., Eriguchi, M.
  • Journal Title: Biomed Pharmacother 63 Pages: 275-286
  • Peer Reviewed
• [Journal Article] Development of monoclonal antibodies for analyzing immune and hematopoietic systems of common marmoset (2009)
  • Author(s): Kametani, Y., Suzuki, D., Tani, K., et al.,
  • Journal Title: Exp Hematol 37 Pages: 1318-1329
  • Peer Reviewed
• [Journal Article] A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo (2009)
  • Author(s): Thacker EE, Nakayama M, Tani. K., et al.,
  • Journal Title: Vaccine 27 Pages: 7116-7124
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 谷憲三朗
  • Journal Title: 遺伝子治療(日本医師会雑誌) Pages: 144-146
• [Journal Article] Non-transmissible Sendai virus encoding granulocyte macrophage colony-stimulating factor is a novel and potent vector system for producing autologous tumor vaccines. (2008)
  • Author(s): Inoue, H., Iga, M., Nabeta, H., Yokoo, T., Suehiro, Y., Okano, S., Inoue, M., Kinoh, H., Katagiri, T., Takayama, K., Yonemitsu, Y., Hasegawa, M., Nakamura, Y., Nakanishi, Y., Tani, K.
  • Journal Title: Cancer Sci. 99(11) Pages: 2315-2326
  • NAID: 10024013606
  • Peer Reviewed
• [Journal Article] TARC and RANTES enhance antitumor immunity induced by the GM-CSF-transduced tumor vaccine in a mouse tumor model. (2008)
  • Author(s): Inoue, H., Iga, M., Xin, M., Asahi, S.,Nakamura, T., Kurita, R., Nakayama, M., Nakazaki, Y., Takayama, K.Nakanishi, Y., Tani, K.
  • Journal Title: Cancer Immunol Immun. 57 Pages: 1399-1411
  • Peer Reviewed
• [Journal Article] Construction of a high-performance human fetal liver-derived lentiviral cDNA library. (2008)
  • Author(s): Kurita, R., Oikawa, T., Okada, M., Yokoo, T., Kurihara, Y., Honda, Y., Kageyama, R., Suehiro, Y., Okazaki, T., Iga, M., Miyoshi, H., Tani, K.
  • Journal Title: Mol Cell Biochem. 319(1-2) Pages: 181-187
  • Peer Reviewed
• [Journal Article] Non-transmissible Sendai virus encoding granulocyte macrophage colony-stimulating factor is a novel and potent vector system for producing autologous tumor vaccines (2008)
  • Author(s): Inoue, H., Iga, M., Tani, K., et al.
  • Journal Title: Cancer Sci 99 Pages: 2315-2326
  • Peer Reviewed
• [Journal Article] TARC and RANTES enhance antitumor immunity induced by the GM-CSF-transduced tumor vaccine in a mouse tumor model (2008)
  • Author(s): Inoue, H., Iga, M., Tani, K., et al.
  • Journal Title: Cancer Immunol Immun 57 Pages: 1399-1411
  • Peer Reviewed
• [Journal Article] Construction of a high-performance human fetal liver-derived lentiviral cDNA library (2008)
  • Author(s): Kurita, R., Tani, K., et al.
  • Journal Title: Mol Cell Biochem 319 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Tumor growth suppression by adenovirus-mediated introduction of a cell growth suppressing gene tob in a pancreatic cancer model (2008)
  • Author(s): Yanagie, H., Tani, K., et al
  • Journal Title: Biomed Pharmacother (in press)
  • Peer Reviewed
• [Presentation] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cells in mice and humans. (2010)
  • Author(s): Inoue T, Tani K, et al.
  • Organizer: American Society of Gene and Cell Therapy, 13^<th> Annual Meeting
  • Place of Presentation: Washington, DC, USA
• [Presentation] A novel coxsackievirus B shows remarkable oncolytic capacity against lung cancer. (2010)
  • Author(s): Miyamoto S, Tani K, et al.
  • Organizer: American Society of Gene and Cell Therapy, 13^<th> Annual Meeting
  • Place of Presentation: Washington, DC, USA
• [Presentation] Establishment of iPS cells derived from the New World monkey of common marmoset. (2010)
  • Author(s): Yamaguchi S, Tani K, et al.
  • Organizer: American Society of Gene and Cell Therapy, 13^<th> Annual Meeting
  • Place of Presentation: Washington, DC, USA
• [Presentation] Coxsackievirus B possesses remarkable oncolytic activity against human lung cancer without lethal side effects. (2010)
  • Author(s): Inoue H, Tani K, et al.
  • Organizer: APSR Travel Grant in the 15^<th> Congress of the Asian Pacific Society of Respirology
  • Place of Presentation: Manila, Philippine
• [Presentation] A novel oncolytic virotherapy by coxsackievirus B against human lung cancer without severe adverse events. (2010)
  • Author(s): Inoue H, Tani K, et al.
  • Organizer: 第16回日本遺伝子治療学会
  • Place of Presentation: 宇都宮
• [Presentation] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cell. (2010)
  • Author(s): Inoue T, Tani K, et al.
  • Organizer: 第16回日本遺伝子治療学会
  • Place of Presentation: 宇都宮
• [Presentation] 悪性腫瘍に対する免疫遺伝子・細胞療法の現状と展望 (2010)
  • Author(s): 谷憲三朗
  • Organizer: 第72回日本血液学会総会
  • Place of Presentation: 横浜
• [Presentation] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cells in mice and humans. (2010)
  • Author(s): Inoue T, et al
  • Organizer: American Society of Gene and Cell Therapy, 13^<th> Annual Meeting
  • Place of Presentation: Washington, DC USA
• [Presentation] A novel coxsackievirus B shows remarkable oncolytic capacity against lung cancer (2010)
  • Author(s): Miyamoto S, et al
  • Organizer: American Society of Gene and Cell Therapy, 13^<th> Annual Meeting
  • Place of Presentation: Washington, DC USA
• [Presentation] Establishment of iPS cells derived from the New World monkey of common marmoset (2010)
  • Author(s): Yamaguchi S, et al
  • Organizer: American Society of Gene and Cell Therapy, 13^<th> Annual Meeting
  • Place of Presentation: Washington, DC USA
• [Presentation] Coxsackievirus B possesses remarkable oncolytic activity against human lung cancer without lethal side effects (2010)
  • Author(s): Inoue H, et al
  • Organizer: APSR Travel Grant in the 15^<th> Congress of the Asian Pacific Society of Respirology
  • Place of Presentation: Manila, Philippine
• [Presentation] A novel oncolytic virotherapy by coxsackievirus B against human lung cancer without severe adverse events. (2010)
  • Author(s): Inoue H, et al
  • Organizer: 第16回 日本遺伝子治療学会
  • Place of Presentation: 宇都宮
• [Presentation] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cell (2010)
  • Author(s): Inoue T, et al
  • Organizer: 第16回 日本遺伝子治療学会
  • Place of Presentation: 宇都宮
• [Presentation] Expression Cloning of Gens Enabling Erythropoietin-Independent Erythropoiesis in Vitro. (2009)
  • Author(s): 横尾朋子, 栗田良, 谷憲三朗, et al.
  • Organizer: American Society of Hematology 51^<th> Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Expression Cloning of Genes Enabling Erythropoietin-Independent Erythronoiesis in Vitro (2009)
  • Author(s): Yokoo T., Kuruta R., Tani K., et al.,
  • Organizer: 51^<th> ASH(American Society of Hematology)
  • Place of Presentation: New Orleans, LA
• [Presentation] ヒト胎児レンチウイルス発現ライブラリーを用いたエリスロポエチン非依存的な赤芽球増殖因子の探索 (2008)
  • Author(s): 横尾朋子、栗田良、谷憲三朗
  • Organizer: 第70回日本血液学会総会
  • Place of Presentation: 京都
• [Presentation] ヒト胎児肝レンチウイルス発現ライブラリーを用いたェリスロポェチン非依存的な赤芽球増殖因子の探索 (2008)
  • Author(s): 横尾朋子, 栗田良, 谷憲三朗
  • Organizer: 第70回 日本血液学会総会
  • Place of Presentation: 京都
• [Book] 悪性腫瘍に対する免疫遺伝子・細胞療法の現状と今後の課題、胆と膵 (2011)
  • Author(s): 谷憲三朗
  • Total Pages: 10
  • Publisher: 医学図書出版
• [Book] 日本血液学会 悪性腫瘍に対する免疫遺伝子・細胞療法の現状と展望(臨床血液) (2010)
  • Author(s): 谷憲三朗
  • Publisher: 社団法人
• [Book] 日本血液学会 抗腫瘍免疫療法における新戦略-New strategies of immunetherapy targeting malignancy-(臨床血液) (2010)
  • Author(s): 村橋(伊賀)睦了
  • Publisher: ら社団法人
• [Book] サイトカインと癌治療(臨床検査) (2010)
  • Author(s): 井上博之、谷憲三朗
  • Publisher: 医学書院
• [Book] 悪性腫瘍に対する免疫遺伝子・細胞療法の現状と今後の課題(胆と膵) (2010)
  • Author(s): 谷憲三朗
  • Publisher: 医学図書出版
• [Book] 悪性腫瘍に対する免疫遺伝子・細胞療法の現状と展望 臨床血液 (2010)
  • Author(s): 谷憲三朗
  • Total Pages: 7
  • Publisher: 社団法人 日本血液学会
• [Book] 抗腫瘍免疫療法における新戦略-New strategies of immunetherapy targeting malignancy-、臨床血液 (2010)
  • Author(s): 村橋(伊賀)睦了, 谷憲三朗, 他
  • Total Pages: 7
  • Publisher: 社団法人 日本血液学会
• [Book] サイトカインと癌治療 臨床検査 (2010)
  • Author(s): 井上博之、谷憲三朗
  • Total Pages: 6
  • Publisher: 医学書院
• [Book] 遺伝子治療(日本医師会雑誌) (2009)
  • Author(s): 谷憲三朗
• [Book] コモンマーモセット (2009)
  • Author(s): 谷憲三朗
  • Publisher: 最新医学社
• [Book] コモンマーモセット (2009)
  • Author(s): 谷 憲三朗
  • Total Pages: 796
  • Publisher: 最新医学社
• [Book] 遺伝子治療領域における細胞療法の現状 (2008)
  • Author(s): 谷憲三朗
  • Publisher: 医薬ジャーナル社
• [Book] 遺伝子治療領域における細胞療法の現状 (2008)
  • Author(s): 谷 憲三朗
  • Total Pages: 115
  • Publisher: 医薬ジャーナル社
• [Remarks] ホームページ等
  • URL: http://www.bioreg.kyushu-u.ac.jp/
• [Patent(Industrial Property Rights)] NOVEL ERYTHROCYTE DIFFERENTIATION INDUCING METHOD AND NOVEL ERYTHROCYTE DIFFERENTIATION MARKER (2010)
  • Inventor(s): 谷憲三朗, 横尾朋子
  • Industrial Property Rights Holder: 九州大学
  • Filing Date: 2010-03-04
  • Overseas
• [Patent(Industrial Property Rights)] 遺伝子治療用ウイルスベクター (2008)
  • Inventor(s): 谷憲三朗, ほか4名
  • Industrial Property Rights Holder: 谷憲三朗, ほか4名
  • Filing Date: 2008-04-25
• [Patent(Industrial Property Rights)] 遺伝子治療用ウイルスベクター (2008)
  • Inventor(s): 谷 憲三朗, 他4名
  • Industrial Property Rights Holder: 谷 憲三朗, 他4名
  • Filing Date: 2008-04-25