Construction of the system for hematopoietic cell production from human ES cells and the analysis of its molecular basis toward the development of ES cell therapies
| Project/Area Number |
20390273
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kyushu University |
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Principal Investigator |
TANI Kenzaburo Kyushu University, 生体防御医学研究所, 教授 (00183864)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Daisuke 九州大学, 医学研究院, 特任准教授 (00426652)
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| Co-Investigator(Renkei-kenkyūsha) |
KURITA Ryo 理化学研究所, バイオリソースセンター, 研究員 (90380526)
YOKOO Tomoko 九州大学, 生体防御医学研究所, 研究員 (70596840)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Keywords | 血液内科学 / 霊長類ES細胞 / Tal1/Scl遺伝子 / レンチウイルスベクター / 造血細胞分化誘導法 / CD34陽性細胞 / 染色体非挿入型ウイルス / 麻疹ウイルス / 幹細胞 / ヒト胚性幹細胞 / コモンマーモセット / Tall / Scl遺伝子 / NOGマウス / GFP遺伝子 / 麻疹ウイルスベクター / VSV-Gシュードタイプレンチウイルスベクター / ヒト胎児肝臓cDNA発現レンチウイルスベクターラブラリー / コモンマーモセットES細胞 / Tal1 / ヒト胎児肝臓 / LVベクターライブラリー |
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| Research Abstract |
In this study, we analyzed the mechanism for hematopoietic cell differentiation by Tal1/Scl gene-transduction for the purpose of developing new therapies using human embryonic stem(ES) cells. We established the ES cell line expressing Tal1/Scl gene constitutively by lentiviral gene transfer and demonstrated that these cell lines produced various types of hematopoietic progenitor cell colonies. And we screened the candidate genes which promote the hematopoiesis as the collaboration with Tal1/Scl gene using high-performance human fetal liver-derived lentiviral cDNA library. We have cloned twenty-nine candidate genes and are now studying their roles in hematopoiesis precisely. These results would give us important information toward the clinical application of human ES cells in future.
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] APOA-1 is a novel marker of erythroid cell maturation from hematopoietic stem cells in mice and humans.2011
Author(s)
Inoue T, Sugiyama D, Kurita R, Oikawa T, Kulkeaw K, Kawano H, Miura Y, Okada M, Suehiro Y, Takahashi A, Marumoto T, Inoue H, Komatsu N, Tani K.
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Journal Title
Stem Cell Rev Rep 7
Pages: 43-52
Related Report
Peer Reviewed
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[Journal Article] cells are specifically enriched in a unique CD3^<dim>CD7^<low> subpopulation of CD4^+ T cells in acute-type adult T cell leukemia.2010
Author(s)
Tian, Y., Kobayashi, S., Ohno, N., Isobe, M., Tsuda, M., Zaike, Y., Watanabe, N., Tani, K.,Tojo, A., Uchimaru, K.Leukemic T
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] CD30 ligand is a target for a novel biological therapy against colitis associated with Th17 responses./CD30 plays a critical role in Th17 differentiation in mice.2010
Author(s)
Sun, X., Yamada, H., Shibata, K., Muta, H., Tani, K., Podack, ER., Iwakura, Y., Yoshikai, Y.
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Journal Title
J Immunol 185
Pages: 7671-7680
Related Report
Peer Reviewed
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[Journal Article] Enhanced Antitumor Effects of an Oncolytic Measles Virus Vaccine Strain Expressing the Wild-type N, P, L Genes on Human Renal Cell Carcinoma2010
Author(s)
Meng, X., Nakamura, T., Okazaki, T., Inoue, H., Takahashi, A., Miyamoto, S., Sakaguchi, G., Eto, M., Naito, S., Takeda, M., Yanagi, Y., Tani, K.
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Journal Title
Mol Ther. 18(3)
Pages: 544-51
Related Report
Peer Reviewed
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[Journal Article] Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinomaantigen 1 promoter-driven oncolytic adenovirus.2010
Author(s)
Hamada, K., Zhang, T., Desaki, J., Nakashiro, K-I., Ito, H., Tani, K., Koyama, Y.Hamakawa, H.
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Journal Title
J.Gene Med. 12
Pages: 545-554
Related Report
Peer Reviewed
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[Journal Article] CD30 ligand/CD30 plays a critical role in Th17 differentiation in mice.2010
Author(s)
Sun, X., Yamada, H., Shibata, K., Muta, H., Tani, K., Podack, ER., Yoshikai, Y.
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Journal Title
J Immunol 185
Pages: 2222-2230
Related Report
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[Journal Article] Tumor growth suppression by adenovirus-mediated introduction of a cell growth suppressing gene tob in a pancreatic cancer model.2009
Author(s)
Yanagie, H., Tanabe, T., Sumimoto, H., Sugiyama, H., Matsuda, S., Nonaka, Y., Ogiwara, N., Sasaki, K., Tani, K.,Takamoto, S., Tkahashi, H., Eriguchi, M.
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Journal Title
Biomed Pharmacother 63
Pages: 275-286
Related Report
Peer Reviewed
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[Journal Article] Non-transmissible Sendai virus encoding granulocyte macrophage colony-stimulating factor is a novel and potent vector system for producing autologous tumor vaccines.2008
Author(s)
Inoue, H., Iga, M., Nabeta, H., Yokoo, T., Suehiro, Y., Okano, S., Inoue, M., Kinoh, H., Katagiri, T., Takayama, K., Yonemitsu, Y., Hasegawa, M., Nakamura, Y., Nakanishi, Y., Tani, K.
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Journal Title
Cancer Sci. 99(11)
Pages: 2315-2326
NAID
Related Report
Peer Reviewed
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[Journal Article] TARC and RANTES enhance antitumor immunity induced by the GM-CSF-transduced tumor vaccine in a mouse tumor model.2008
Author(s)
Inoue, H., Iga, M., Xin, M., Asahi, S.,Nakamura, T., Kurita, R., Nakayama, M., Nakazaki, Y., Takayama, K.Nakanishi, Y., Tani, K.
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Journal Title
Cancer Immunol Immun. 57
Pages: 1399-1411
Related Report
Peer Reviewed
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[Journal Article] Construction of a high-performance human fetal liver-derived lentiviral cDNA library.2008
Author(s)
Kurita, R., Oikawa, T., Okada, M., Yokoo, T., Kurihara, Y., Honda, Y., Kageyama, R., Suehiro, Y., Okazaki, T., Iga, M., Miyoshi, H., Tani, K.
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Journal Title
Mol Cell Biochem. 319(1-2)
Pages: 181-187
Related Report
Peer Reviewed
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