A new strategy for antibody-therapy against infectious diseases and bacterial terrorism using lymphocyte-chip

• Project/Area Number: 20390286
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: University of Toyama
• Principal Investigator: MURAGUCHI Atsushi University of Toyama, 大学院・医学薬学研究部, 教授 (20174287)
• Co-Investigator(Kenkyū-buntansha): KISHI Hiroyuki 富山大学, 大学院・医学薬学研究部, 准教授 (60186210) ; OZAWA Tatsuhiko 富山大学, 大学院・医学薬学研究部, 助教 (10432105) ; KITAJIMA Isao 富山大学, 大学院・医学薬学研究部, 教授 (50214797) ; NIKAIDO Toshio 富山大学, 大学院・医学薬学研究部, 教授 (50180568)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: リンパ球チップ / 抗体医療 / 抗体遺伝子 / 抗体産生細胞 / リコンビナント抗体 / インフルエンザウイルス / B型肝炎ウイルス / 中和抗体 / 抗体医薬 / 感染症 / 抗体工学 / ヒト抗体 / 新興感染症

Research Abstract

We developed ISAAC (Immunospot assay for antibody-secreting cell) method that can detect antibody-secreting cells in a microwell using murine system. We then succeeded in generating high-quality human monoclonal antibodies for hepatitis B virus and influenza virus from vaccinated healthy volunteers using the ISAAC method in a very short time ; within one week. We further developed an in vitro assay system to examine the HBV-neutralizing activity of human anti-HBs antibodies and clarified the relationship between neutralizing activity of human mAbs and epitopes of HBs antigen.

Research Products

• [Journal Article] Aberrant CaMKII activity is associated with abnormal dendoric spine morphology in ATRX mutant mouse brain. (2011)
  • Author(s): Shioda N., et al
  • Journal Title: J Neurosci Volume: 31 Pages: 346-358
  • Peer Reviewed
• [Journal Article] Generation of TRAIL-receptor 1-specific human monoclonal Ab by a combination of immunospot array assay on a chip and human Ab-producing mice. (2010)
  • Author(s): Jin A., Ozawa T., Tajiri K., Lin Z., Obata T., Ishida I., Kishi H., Muraguchi A.
  • Journal Title: Eur.J.Immunol. 40 Pages: 3591-3593
  • Peer Reviewed
• [Journal Article] Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine. (2010)
  • Author(s): Tajiri K., Ozawa T., Jin A., Tokimitsu Y., Minemura M., Kishi H., Sugiyama T., Muraguchi A.
  • Journal Title: Antiviral.Res. 87 Pages: 40-49
  • Peer Reviewed
• [Journal Article] Post-translational modification of TRAIL receptor type 1 on various tumor cells and the susceptibility of tumors to TRAIL-induced apoptosis. (2010)
  • Author(s): Lin Z., Jin A., Ozawa T., Tajiri K., Obata T., Ishida I., Jin F., Kishi H., Muraguchi A.
  • Journal Title: Biochem.Biophys.Res.Commun. 395 Pages: 251-257
  • Peer Reviewed
• [Journal Article] Generation of TRAIL-receptor 1-specific human monoclonal Ab by a combination of immunospot array assay on a chip and human Ab-producing mice. (2010)
  • Author(s): Jin A., et al
  • Journal Title: Eur.J.Immunol. Volume: 40 Pages: 3591-3593
  • Peer Reviewed
• [Journal Article] Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine. (2010)
  • Author(s): Tajiri K., et al
  • Journal Title: Antiviral.Res. Volume: 87 Pages: 40-49
  • Peer Reviewed
• [Journal Article] Post-translational modification of TRAIL receptor type 1 on various tumor cells and the susceptibility of tumors to TRAIL-induced apoptosis. (2010)
  • Author(s): Lin Z., et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 395 Pages: 251-257
  • Peer Reviewed
• [Journal Article] Regulatory role of leukocyte common antigen-related molecule (LAR) in thymocyte differentiation. (2010)
  • Author(s): Kondo S., et al
  • Journal Title: Eur.J.Immunol. Volume: 40 Pages: 1296-1302
  • Peer Reviewed
• [Journal Article] Venous thromboembolism after spinal surgery : Changes of the fibrin monomer complex and D-dimer level during the perioperative period. (2010)
  • Author(s): Yoshioka K., et al
  • Journal Title: Journal of Neurosurgery.Spine Volume: 13 Pages: 594-599
  • Peer Reviewed
• [Journal Article] Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice. (2010)
  • Author(s): Nogami T., et al
  • Journal Title: HIPPOCAMPUS Volume: 21巻
  • Peer Reviewed
• [Journal Article] A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour. (2010)
  • Author(s): Ito M., et al
  • Journal Title: J Reprod Immunol Volume: 84 Pages: 75-85
  • Peer Reviewed
• [Journal Article] Cyclin A2 confers cisplatin resistance to endometrial carcinoma cells via up-regulation of an Akt-binding protein, periplakin. (2010)
  • Author(s): Suzuki A., et al
  • Journal Title: J Cell Mol Med Volume: 14 Pages: 2305-2317
  • Peer Reviewed
• [Journal Article] A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour, (2010)
  • Author(s): Ito M, et al.
  • Journal Title: J Reprod Immunol 84 Pages: 75-85
  • Peer Reviewed
• [Journal Article] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. (2009)
  • Author(s): Jin A.-S., Ozawa T., Tajiri K., Obata T., Kondo S., Kinoshita K., Kadowaki S., Takahashi K., Sugiyama T., Kishi H., Muraguchi A.
  • Journal Title: Nat.Med. 15 Pages: 1088-1092
  • Peer Reviewed
• [Journal Article] Identification of antigen-specific B cells by concurrent monitoring of intracellular Ca^<2+> mobilization and antigen binding with microwell array chip system equipped with a CCD imager. (2009)
  • Author(s): Kinoshita K., Ozawa T., Tajiri K., Kadowaki S., Kishi H., Muraguchi A.
  • Journal Title: Cytometry Part A 75A Pages: 682-687
  • Peer Reviewed
• [Journal Article] SFMAC : A novel method for analyzing multiple parameters on lymphocytes with a single fluorophore in cell-microarray system. (2009)
  • Author(s): Tajiri K., Kishi H., Ozawa T., Sugiyama T., Muraguchi A.
  • Journal Title: Cytometry Part A 75A Pages: 282-288
  • Peer Reviewed
• [Journal Article] MAC-CCD system : a novel lymphocyte microwell-array chip system equipped with CCD scanner to generate human monoclonal antibodies against influenza virus. (2009)
  • Author(s): Ozawa T., Kinoshita K., Kadowaki S., Tajiri K., Kondo S., Honda R., Ikemoto M., Piao L.-X., Morisato A., Fukurotani K., Kishi H., Muraguchi A.
  • Journal Title: Lab on a Chip 9 Pages: 158-163
  • Peer Reviewed
• [Journal Article] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. (2009)
  • Author(s): Jin A.-S., et al
  • Journal Title: Nature Medicine 15 Pages: 1088-1092
  • Peer Reviewed
• [Journal Article] Identification of antigen-specific B cells by concurrent monitoring of intracellular Ca^<2+> mobilization and antigen binding with microwell array chip system equipped with a CCD imager. (2009)
  • Author(s): Kinoshita K., et al
  • Journal Title: Cytometry Part A 75A Pages: 682-687
  • Peer Reviewed
• [Journal Article] Induction of autophagic cell death and radiosensitization by the pharmacological inhibition of nuclear factor-kappa B activation in human glioma cell lines. (2009)
  • Author(s): Tsuoi Y, et al.
  • Journal Title: J.Neurosurg. 110 Pages: 594-604
  • Peer Reviewed
• [Journal Article] MAC-CCD system : a novel lymphocyte microwell-array chip system equipped with CCD scanner to generate human monoclonal antibodies against influenza virus (2009)
  • Author(s): Ozawa T., et al
  • Journal Title: Lab on a Chip 9 Pages: 158-163
  • Peer Reviewed
• [Journal Article] SFMAC : A novel method for analyzing multiple parameters on lymphocytes with a single fluorophore in cell-microarray system (2009)
  • Author(s): Tajiri K., et al
  • Journal Title: Cytometry Part A 75A Pages: 282-288
  • Peer Reviewed
• [Journal Article] The direct excitatory effect of IL-1β on cerebellar Purkinje cell (2009)
  • Author(s): Motoki K., et al
  • Journal Title: Biochem. Biophys. Res. Commun. 379 Pages: 665-668
  • Peer Reviewed
• [Journal Article] Induction of autophagic cell death and radiosensitization by the pharmacological inhibition of nuclear factor-kappa B actication in human glioma cell lines (2009)
  • Author(s): Tsuboi Y., et al
  • Journal Title: J. Neurosurg 110 Pages: 594-604
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of the functional TCR repertoire at the single-cell level (2008)
  • Author(s): Ozawa T., et al
  • Journal Title: Biochem. Biophys. Res. Commun. 367 Pages: 820-825
  • Peer Reviewed
• [Journal Article] Effects of different statins on endothelial nitric oxide synthase and AKT phosphorylation in endothelial cells (2008)
  • Author(s): Wang J., et al
  • Journal Title: International Journal of Cardiology 127 Pages: 33-39
  • Peer Reviewed
• [Journal Article] Changes of the soluble fibrin monomer complex level in the perioperative period of hip replacement surgery (2008)
  • Author(s): Misaki T., et al
  • Journal Title: J. Orthop. Sci. 13 Pages: 419-424
  • Peer Reviewed
• [Journal Article] Characterization of laminin isoforms in human amnion (2008)
  • Author(s): Takashima S., et al
  • Journal Title: Tissue and Cell 40 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Overexpression of RhoA enhances peritoneal dissemination. RhoA suppression with Lovastatin may be useful for ovarian cancer (2008)
  • Author(s): Horiuchi A., et al
  • Journal Title: Cancer Sci. 99 Pages: 2532-2539
  • Peer Reviewed
• [Presentation] Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer. (2011)
  • Author(s): Muraguchi A., Ozawa T., Jin A., Tajiri K., Takemoto M., Okuda T., Shiraki K., Kishi H.
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone, USA.
• [Presentation] Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer. (2011)
  • Author(s): Muraguchi A., et al
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone, USA
• [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-Receptor 1 specific human mAbs generated by Immunos of array assay on a chip. (2011)
  • Author(s): Jin A., et al
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone, USA
• [Presentation] Nuclear factor-kappa B activity correlates with metabolic syndrome-related biomerkers in patients with essential hypertension. (2010)
  • Author(s): Harada K., et al
  • Organizer: 2010 AACC (American Association for Clinical Chemistry)
  • Place of Presentation: Anaheim, CA, USA
  • Year and Date: 2010-07-27
• [Presentation] Anti-inflammatory effect of pitavastatin on NF-kaoppa B activated by TNF-alfa through Rho kinase pathway in breast cancer cells. (2010)
  • Author(s): Kitajima I
  • Organizer: Keystone Symposia 2010 NF-kappa B in inflammation and Disease
  • Place of Presentation: Santa Fe, USA
  • Year and Date: 2010-01-08
• [Presentation] Isolation and characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus. (2010)
  • Author(s): Ozawa T., Jin A., Tajiri K., Takemoto M., Okuda T., Shiraki K., Kishi H., Muraguchi A.
  • Organizer: Cold Spring Harbor Conferences Asia
  • Place of Presentation: Suzhou, China.
• [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-receptor 1 specific human mAbs generated by Immunospot array assay on a chip. (2010)
  • Author(s): Jin A., Ozawa T., Tajiri K., Lin Z., Kishi H., Muraguchi A.
  • Organizer: Cold Spring Harbor Conferences Asia
  • Place of Presentation: Suzhou, China.
• [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-R1-specific human mAbs generated by immunospot array assay on a chip. (2010)
  • Author(s): Kishi H., Jin A., Ozawa T., Tajiri K., Lin Z., Obata T., Ishida I., Muraguchi A.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe.
• [Presentation] Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer. (2010)
  • Author(s): Ozawa T., Jin A., Tajiri K., Shiraki K., Kishi H., Muraguchi A.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe.
• [Presentation] Detection of antigen-specific T-cell response on the single cell-basis with microwell array chip. (2010)
  • Author(s): Kishi H., Tajiri K., Hatakeyama S., Kobayashi E., Horii M., Jin A., Ozawa T., Muraguchi A.
  • Organizer: 4th Measuring Antigen-Specific Immune Response (MASIR) Conference
  • Place of Presentation: Mykonos, Greece.
• [Presentation] Detection of antigen-specific T-cell response on the single cell-basis with microwell array chip. (2010)
  • Author(s): Kishi H., et al
  • Organizer: 4th Measuring Antigen- Specific Immune Response (MASIR) Conference
  • Place of Presentation: Mykonos, Greece
• [Presentation] Rapid and efficient cloning and functional evaluation system for antigen- specific T cell receptor obtained from single T-cells. (2010)
  • Author(s): Horii M., et al
  • Organizer: 4th Measuring Antigen-Specific Immune Response (MASIR) Conference
  • Place of Presentation: Mykonos, Greece
• [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-R1-specific human mAbs generated by immunospot array assay on a chip. (2010)
  • Author(s): Kishi H., et al
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer. (2010)
  • Author(s): Ozawa T., et al
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] The novel method to detect single cytokine-secreting T-cell using a cell-microarray chip. (2010)
  • Author(s): Tajiri K., et al
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] Single T cell analysis system for rapid cloning and functional evaluation of antigen-specific T cell receptors. (2010)
  • Author(s): Kobayashi E., et al
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] Isolation and characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus. (2010)
  • Author(s): Ozawa T., et al
  • Organizer: Cold Spring Harbor Conferences Asia
  • Place of Presentation: Suzhou, China
• [Presentation] Enhancement of TRAIL-induced apoptosis with novel TRAIL-receptor 1 specific human mAbs generated by Immunos of arra assay on a chip. (2010)
  • Author(s): Jin A., et al
  • Organizer: Cold Spring Harbor Conferences Asia
  • Place of Presentation: Suzhou, China
• [Presentation] ユニバーサルワクチンに向けたHBワクチンの作用機序の検討 (2010)
  • Author(s): 田尻和人, 他
  • Organizer: 第46回肝臓学会総会
  • Place of Presentation: 山形
• [Presentation] ヒトから単離したインフルエンザM2e特異的抗体の機能解析 (2010)
  • Author(s): 小澤龍彦, 他
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
• [Presentation] Characterization of amniotic stem cells. (2010)
  • Author(s): Koike C., et al
  • Organizer: The 8th International Society for Stem Cell Research
  • Place of Presentation: San Francisco
• [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. (2009)
  • Author(s): 小澤龍彦, 金艾順, 田尻和人, 小幡勤, 近藤佐千子, 木下耕史, 門脇慎一, 高橋和郎, 杉山敏郎, 岸裕幸, 村口篤
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. (2009)
  • Author(s): 金艾順, 小澤龍彦, 田尻和人, 岸裕幸, 村口篤
  • Organizer: IBC's 20th Annual International Conference Antibody Engineering
  • Place of Presentation: San Diego, USA.
• [Presentation] 単一細胞レベルにおけるB細胞受容体シグナル解析 (2009)
  • Author(s): 岸裕幸, 小澤龍彦, 田尻和人, 村口篤
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] Isolation of human anti-influenza M2 antibody from peripheral blood of healthy volunteer. (2009)
  • Author(s): 小澤龍彦, 田尻和人, 金艾順, 岸裕幸, 村口篤
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. (2009)
  • Author(s): 金艾順, 小澤龍彦, 田尻和人, 岸裕幸, 村口篤
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] Extracellular loop domain of small (S)-HBsAg play a major role in preventing the infection of HBV : the result from omprehensive analysis of monoclonal antibodies obtained from S-HBsAg-vaccinated healthy individuals. (2009)
  • Author(s): Tajiri K., Ozawa T., Jin A.-S., Tokimitsu Y., Nakayama Y., Minemura M., Kishi H., Sugiyama T., Muraguchi A.
  • Organizer: The 8th JSH Single Topic Conference
  • Place of Presentation: Tokyo.
• [Presentation] B-cell receptor signal analysis at single cell levels using a microwell-array chip. (2009)
  • Author(s): Kinoshita K., Kishi H., Fukurotani K., Honda R., Ozawa T., Tajiri K., Muraguchi A.
  • Organizer: Cold Spring Harbor Meeting
  • Place of Presentation: New York.
• [Presentation] B-cell receptor signal analysis at single cell levels using a microwell-array chip. (2009)
  • Author(s): Kishi H., et al
  • Organizer: Cold Spring Harbor Meeting
  • Place of Presentation: New York
• [Presentation] Extracellular loop domain of small (S)-HBsAg play a major role in preventing the infection of HBV : the result from comprehensive analysis of monoclonal antibodies obtained from S-HBsAg-vaccinated healthy individuals. (2009)
  • Author(s): Tajiri K., et al
  • Organizer: The 8th JSH Single Topic Conference
  • Place of Presentation: Tokyo
• [Presentation] A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. (2009)
  • Author(s): Muraguchi A., et al
  • Organizer: IBC's 20th Annual International Conference Antibody Engineering
  • Place of Presentation: San Diego
• [Presentation] HBワクチンにより得られたモノクローナル抗体のHBV中和活性とエピトープの解析 (2009)
  • Author(s): 田尻和人, 他
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] Microwell arrays for detecting single, antigen-specific B-lineage cells. (2008)
  • Author(s): 岸裕幸
  • Organizer: IBC's 19th Annual International Conference Antibody Engineering
  • Place of Presentation: San Diego.
• [Presentation] マイクロウェルアレイチップを用いた抗原特異的T細胞の検出 (2008)
  • Author(s): 岸裕幸, 田尻和人, 小澤龍彦, 金艾順, 村口篤
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] Microwell arrays for detecting single, antigen-specific B-lineage cells (2008)
  • Author(s): Kishi H.
  • Organizer: IBC's 19th Annual International Conference Antibody Engineering
  • Place of Presentation: San Diego
• [Presentation] HBワクチンにより誘導されるHBsAg特異的モノクローナル抗体についての検討 (2008)
  • Author(s): 田尻 和人, 他
  • Organizer: 第44回日本肝臓学会総会
  • Place of Presentation: 松山
• [Presentation] Isolation of human anti-Influenza virus antibodies secreting cells from peripheral blood of Influenza HA-vaccinated healthy volunteer (2008)
  • Author(s): 小澤 龍彦, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] リンパ球チップを用いた完全ヒト抗体産生マウス(TCマウス)からのヒト抗TRAIL-R1抗体遺伝子取得 (2008)
  • Author(s): 金 艾順, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] Hepatitis B virus-neutralization activity with a panel of human monoclonal antibodies obtained by cell-microarray system (2008)
  • Author(s): 田尻 和人, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] MAC-CCD systemを用いた細胞内カルシウムの時系列解析とヒトモノクローナルインフルエンザ抗体の単離 (2008)
  • Author(s): 小澤 龍彦, 他
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 植物細胞を利用したヒトB型肝炎ウイルス抗体の作製及び機能解析 (2008)
  • Author(s): 池本 守, 他
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Role of Nuclear factor-kappa B (NF-κB) in tumor progression (2008)
  • Author(s): Kitajima I.
  • Organizer: The 1^<st> International traditional Chinese medicine and Interactive Medicine Oncology Forum
  • Place of Presentation: Shanghai
• [Presentation] mRNA and micro RNA expression profile in chondrogenic differentiation regulated by BMP (2008)
  • Author(s): Kitajima I., et al
  • Organizer: Annual TGF-β meeting Leiden
  • Place of Presentation: Leiden
• [Presentation] Gas 6, a new regulator of chodrogenic differentiation promoted by BMP (2008)
  • Author(s): Kitajima I., et al
  • Organizer: Annual TGF-β meeting Leiden
  • Place of Presentation: Leiden
• [Presentation] The Potential of Amniotic Membrane (2008)
  • Author(s): Nikaido T.
  • Organizer: The 2nd Hong Kong International Burns & Wound Healing Symposium
  • Place of Presentation: Hong Kong
• [Presentation] Amnion-Derived Cells for Regenerative Medicine (2008)
  • Author(s): Nikaido T.
  • Organizer: The 2nd Hong Kong International Burns & Wound Healing Symposium
  • Place of Presentation: Hong Kong
• [Presentation] Application of Amniotic Membrane/Amnion-Derived Cells for Regenerative Medicine (2008)
  • Author(s): Nikaido T.
  • Organizer: The 5^<th> Chinese Conference on Oncology
  • Place of Presentation: Shijiazhuang