Sensitive prion detection by in vitro amplification of abnormal prion protein
Project/Area Number |
20390287
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Infectious disease medicine
|
Research Institution | Nagasaki University |
Principal Investigator |
ATARASHI Ryuichiro Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (90452846)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
|
Keywords | 感染症診断学 / プリオン病 / プリオン / 異常型プリオンタンパク / 試験管内増幅法 / リコンビナントタンパク / 早期診断 |
Research Abstract |
The development of in vitro amplification technology for abnormal forms of prion protein (PrPSc) has generated the possibility for developing a novel diagnostic test for prion diseases. However, ultrasensitive PrPSc detection in easily accessible specimens such as cerebrospinal fluid (CSF) has not yet been successful in human prion diseases. In this study, we developed a new PrPSc amplification assay, designated "real-time QUIC (RT-QUIC)", allows for the detection of a minute amount of PrPSc in diluted sporadic Creutzfeldt?Jakob disease (sCJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.
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Report
(4 results)
Research Products
(25 results)