Establishment of a method inducing embryonic stem cells to differentiate to hematopoietic stem cells (HSC) for therapeutic HSC transplantation
| Project/Area Number |
20390291
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TSUJI Kohichiro The University of Tokyo, 医科学研究所, 准教授 (50179991)
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| Co-Investigator(Kenkyū-buntansha) |
EBIHARA Yasuhiro 東京大学, 医科学研究所, 助教 (40302608)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | ヒト胚性幹細胞(ES細胞) / 造血幹細胞 / ストローマ細胞 / 再生医療 / ヒトES細胞 / 多能性造血細胞 / バイオテクノロジー / 間葉系幹細胞 / 移植 / ヒト胚性幹細胞 / 造血幹細胞. / 胎仔肝 / ヒト血清 / ヒト血漿. / platelet lysate |
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| Research Abstract |
We challenged to establish a method inducing human embryonic stem (ES) cells to differentiate into hematopoietic stem cells without animal cells and serum for their clinical application. In brief, we first transferred human ES cells onto gelatin-coated culture dishes and cultured with 5% human platelet lysate or serum-containing media to induce their differentiation into stromal cells. Then, human ES cells were cocultured with the estabshished stromal cells under animal serum-free condition for 2 weeks to generate multipotent hematopoietic cells.Thus, we succeeded the establishment of a novel method inducing human ES cells to differentiate into multipotent hematopoietic cells without anima cells and seru,.
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Report
(4 results)
Research Products
(95 results)
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[Journal Article] Sero-prevalence of pandemic (H1N1) 2009 influenza A virus among schoolchildren and their parents inTokyo, Japan.2011
Author(s)
Iwatsuki-Horimoto K, Horimoto T, Tamura D, Kiso M, Kawakami E, Hatakeyama S, Ebihara Y, Koibuchi T, Fujii T, Takahashi K, Shimojima M, Sakai-Tagawa Y, Ito M, Sakabe S, Iwasa A, Takahashi K, Ishii T, Gorai T, Tsuji K, Iwamoto A, Kawaoka Y.
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Journal Title
J Clinic Microbiol (掲載確定)
Related Report
Peer Reviewed
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[Journal Article] A 5'untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model.2010
Author(s)
Nagamachi A, Htun PW, Ma F, Miyazaki K, Yamasaki N, Kanno M, Inaba T, Honda Z, Okuda T, Oda H, Tsuji K, Honda H H(*equally contributed)
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Journal Title
Dev Biol 345
Pages: 226-236
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Peer Reviewed
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[Journal Article]2009
Author(s)
海老原康榑
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Journal Title
小児がん.がん患者の心に寄り添うためのサイコオンコロジーの基礎と実践.大木桃代編(真興交易医書出版部)
Pages: 43-47
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[Journal Article] Cardiovascular toxicity of cryopreserved cord blood cell infusion.2008
Author(s)
Konuma T, Konuma T, Ooi J, Takahashi S, Tomonari A, Tsukada N, Kobayashi T, Sato A, Kato S, Kasahara S, Ebihara Y, Nagamura-Inoue T, Tsuji K, Tojo A, Asano S.
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Journal Title
Bone Marrrow Transplant 41
Pages: 861-865
Related Report
Peer Reviewed
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[Presentation] 慢性骨髄性白血病に対する骨髄破壊的前処置を用いた臍帯血移植2010
Author(s)
河北敏郎, 大井淳, 高橋聡, 塚田信弘, 加藤せい子, 佐藤亜紀, 永田泰之, 海老原康博, 辻浩一郎, 浅野茂隆, 東條有伸
Organizer
第32回日本造血細胞移植学会
Place of Presentation
浜松
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[Presentation] Second or third myeloablative allo-SCT using cord blood for adult leukemia.2009
Author(s)
加藤せい子, 大井淳, Tahashi S, Tsukada N, Sato A, Konuma T, 河北敏郎, Nagata Y, 海老原康博, 辻浩一郎, 東條有伸, 浅野茂隆.
Organizer
第71回日本血液学会総会
Place of Presentation
京都
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