Generation of hematopoietic cells and immune cells from iPS cells

• Project/Area Number: 20390296
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyoto University
• Principal Investigator: HEIKE Toshio Kyoto University, 大学院・医学研究科, 教授 (90190173)
• Co-Investigator(Kenkyū-buntansha): UMEDA Katsutsugu 京都大学, 大学院・医学研究科, 助教 (80397538) ; BABA Shiro 京都大学, 大学院・医学研究科, 助教 (60432382) ; KATO Takeo 京都大学, 大学院・医学研究科, 助教 (60422945) ; FUJINO Hisanori 京都大学, 大学院・医学研究科, 助教 (70532604)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: ES細胞 / iPS細胞 / 線維芽細胞 / 再生医学 / 造血・免疫細胞 / 繊維芽細胞

Research Abstract

In murine iPS cells, we identified the Flk-1(+) cells as the hemoangiogenic progenitors, "hemangioblasts", differentiated mature erythrocytes and analyzed their functions. We also established a neutrophil differentiation system from human iPS cells. The iPS cell-derived neutrophils were equipped with morphological and functional characteristics of mature neutrophils and traced the differentiation process similar to bone marrow hematopoiesis.

Research Products

• [Journal Article] Neutrophil differentiation from human-induced pluripotent stem cells. (2011)
  • Author(s): Morishima T, Watanabe K, Niwa A, Fujino H, Matsubara H, Adachi S, Suemori H, Nakahata T, Heike T.
  • Journal Title: J Cell Physiol. 226(5) Pages: 1283-1291
  • NAID: 40019064352
  • Peer Reviewed
• [Journal Article] Neutrophil differentiation from human-induced pluripotent stem cells (2011)
  • Author(s): Morishima T, Watanabe K, Niwa A, Fujino H, Matsubara H, Adachi S, Suemori H, Nakahata T, Heike T
  • Journal Title: J Cell Physiol Volume: 226 Pages: 1283-1291
  • NAID: 40019064352
  • Peer Reviewed
• [Journal Article] Cell line-dependent differentiation of induced pluripotent stem cells into cardiomyocytes in mice. (2010)
  • Author(s): Kaichi S, Hasegawa K, Takaya T, Yokoo N, Mima T, Kawamura T, Morimoto T, Ono K, Baba S, Doi H, Yamanaka S, Nakahata T, Heike T.
  • Journal Title: Cardiovasc Res. 88 Pages: 315-322
  • Peer Reviewed
• [Journal Article] Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. (2010)
  • Author(s): Mizuno Y, Chang H, Umeda K, Niwa A, Iwasa T, Awaya T, Fukuda S, Yamamoto H, Yamanaka S, Nakahata T, Heike T
  • Journal Title: FASEB J 24 Pages: 2245-2253
  • Peer Reviewed
• [Journal Article] Cell line-dependent differentiation of induced pluripotent stem cells into cardiomyocytes in mice (2010)
  • Author(s): Kaichi S, Hasegawa K, Takaya T, Yokoo N, Mima T, Kawamura T, Morimoto T, Ono K, Baba S, Doi H, Yamanaka S, Nakahata T, Heike T
  • Journal Title: Cardiovasc Res Volume: 88 Pages: 315-322
  • Peer Reviewed
• [Journal Article] Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells (2010)
  • Author(s): Mizuno Y, Chang H, Umeda K, Niwa A, Iwasa T, Awaya T, Fukuda S, Yamamoto H, Yamanaka S, Nakahata T, Heike T
  • Journal Title: FASEB J (in press)
  • Peer Reviewed
• [Journal Article] Orderly hematopoietic development of induced pluripotent stem cells via Flk-1 (+) hemoangiogenic progenitors. (2009)
  • Author(s): Niwa A, Umeda K, Chang H, Saito M, Okita K, Takahashi K, Nakagawa M, Yamanaka S, Nakahata T, Heike T
  • Journal Title: J Cell Physiol 221 Pages: 367-377
  • Peer Reviewed
• [Journal Article] Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells. (2009)
  • Author(s): Chang H, Yoshimoto M, Umeda K, Iwasa T, Mizuno Y, Fukuda S, Yamamoto H, Motohashi N, Suzuki Y, Takeda S, Heike T, Nakahata T
  • Journal Title: FASEB J 23 Pages: 1907-1919
  • Peer Reviewed
• [Journal Article] Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells (2009)
  • Author(s): Chang H, Yoshimoto M, Umeda K, Iwasa T, Mizuno Y, Fukuda S, Yamamoto H, Motohashi N, Suzuki Y, Takeda S, Heike T, Nakahat T
  • Journal Title: FASEB J 23 Pages: 1907-1919
  • Peer Reviewed
• [Journal Article] Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis. (2008)
  • Author(s): Nagao K, Ohta T, Tahara T, Hasiwara T, Naeda Y, Yoneyaya T, Sohma Y, Heike T, Nakahata T, Inagaki Y, Nishikawa M
  • Journal Title: Biochem Biophys Res Commun 377 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis (2008)
  • Author(s): Nagao K, Ohta T, Tahara T, Hasiwara T, Naeda Y, Yoneyaya T, Sohma Y, Heike T, Nakahata T, Inagaki Y, Nishikawa M
  • Journal Title: Biochem Biophys Res Commun 377 Pages: 205-209
  • Peer Reviewed
• [Presentation] Neutrophil differentiation from human induced pluripotent stem (iPS) cells for disease investigation. (2010)
  • Author(s): Morishima T, Watanabe K, Niwa A, Fujino H, Adachi S, Nakahata T, Heike T
  • Organizer: The 72^<nd> Annual Metting of the Japanese Society of Hematology
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-09-25