| Project/Area Number |
20390308
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
|
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| Research Institution | Kurume University |
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Principal Investigator |
HASHIMOTO Takashi Kurume University, 医学部, 教授 (20129597)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
安元 慎一郎 久留米大学, 医学部, 准教授 (10220162)
辛島 正志 久留米大学, 医学部, 講師 (70211175)
濱田 尚宏 久留米大学, 医学部, 講師 (40320204)
石井 文人 久留米大学, 医学部, 助教 (80330827)
大山 文悟 久留米大学, 医学部, 助教 (90461441)
大日 輝記 久留米大学, 医学部, 講師 (20423543)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | プロテオーム / 免疫学 / 蛋白質 / 遺伝子 / バイオテクノロジー / ケラチノサイト / デスモソーム / ヘミデスモソーム / たんぱく質 |
|---|
| Research Abstract |
We identified the autoantigen for anti-p200 pemphigoid as laminin gamma-1 by proteomics technique, produced recombinant proteins of laminin gamma-1, and established novel ELISA. We established new immunoprecipitation technique for IgA antibodies using protein M sepharose and cultured KU8 cells which were shown to produce plenty of autoantigen for IEN type IgA pemphigus. We identified the autoantigen as IgA secretary component by two-dimensional gel electrophoresis, immunoblotting and mass spectrometric analysis.
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