| Budget Amount *help |
¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Research Abstract |
We characterized 5-HTergic neurons that also contained GAD67 compared to neurons containing only 5-HT through electrophysiological, molecular biological and neuroanatomical methods. We confirmed tryptophan hydroxylase 2 (TPH2) mRNA and GAD67 mRNA co-expression in the DRN via in situ hybridization and single-cell RT-PCR methods. We also demonstrated that GABA is not released into the synaptic cleft of these neurons. In the electrophysiological experiment, we found that the action potential amplitude, membrane resistance and firing frequency of both TPH2 and GAD67 mRNA-positive neurons were all significantly lower than in neurons that were positive for TPH2 mRNA only. An early postnatal stress disturbed the development of TPH2 and GAD67 mRNA-positive neurons.
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