Effects of early postnatal stress on the development of 5-HT/GABA co-localized neuron in the raphe nuclei
| Project/Area Number |
20390309
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Taku 北海道大学, 大学院・医学研究科, 助教 (80325563)
IZUMI Takeshi 北海道大学, 大学院・医学研究科, 助教 (60312360)
YOSHIDA Takayuki 北海道大学, 大学院・医学研究科, 助教 (60374229)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 精神薬理学 / セロトニン / GABA / 縫線核 / 情動 / ストレス / 発達 / 海馬 / 内側前頭前野 / RT-PCR |
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| Research Abstract |
We characterized 5-HTergic neurons that also contained GAD67 compared to neurons containing only 5-HT through electrophysiological, molecular biological and neuroanatomical methods. We confirmed tryptophan hydroxylase 2 (TPH2) mRNA and GAD67 mRNA co-expression in the DRN via in situ hybridization and single-cell RT-PCR methods. We also demonstrated that GABA is not released into the synaptic cleft of these neurons. In the electrophysiological experiment, we found that the action potential amplitude, membrane resistance and firing frequency of both TPH2 and GAD67 mRNA-positive neurons were all significantly lower than in neurons that were positive for TPH2 mRNA only. An early postnatal stress disturbed the development of TPH2 and GAD67 mRNA-positive neurons.
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Report
(4 results)
Research Products
(64 results)
