| Budget Amount *help |
¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Research Abstract |
After heat treatment at 45.5℃ for 20 min, DNA DSB recognizing proteins were partially localized with γH2AX foci. Here we show that there were slight differences in the localization pattern among these proteins, such as a disappearance from the nucleus (phospho-ATM) and translocation to the cytoplasm (phospho-NBS1) at 30 min after heat treatment, and some foci (phospho- DNA-PKcs and phospho-Chk2) appeared at 8 h after heat treatment. In addition, we demonstrated that not NHEJ deficient cells but HR deficient cells is heat-sensitivity compared with these proficient cells. Polβ functions as a critical elements involved in thermotolerance. Moreover, we indicated that heat-induced foci of γH2AX and these DNA DSB recognizing proteins were suppressed in thermotolerance development.
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