Analysis of cell nuclear dynamics in thermotolerance development
Project/Area Number |
20390330
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Radiation science
|
Research Institution | Nara Medical University |
Principal Investigator |
TAKAHASHI Akihisa Nara Medical University, 先端科学研究指導者育成ユニット, 准教授 (60275336)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | 放射線治療学 / 温熱治療 / 温熱耐性 / DNA二本鎖切断 / 細胞核ダイナミクス |
Research Abstract |
After heat treatment at 45.5℃ for 20 min, DNA DSB recognizing proteins were partially localized with γH2AX foci. Here we show that there were slight differences in the localization pattern among these proteins, such as a disappearance from the nucleus (phospho-ATM) and translocation to the cytoplasm (phospho-NBS1) at 30 min after heat treatment, and some foci (phospho- DNA-PKcs and phospho-Chk2) appeared at 8 h after heat treatment. In addition, we demonstrated that not NHEJ deficient cells but HR deficient cells is heat-sensitivity compared with these proficient cells. Polβ functions as a critical elements involved in thermotolerance. Moreover, we indicated that heat-induced foci of γH2AX and these DNA DSB recognizing proteins were suppressed in thermotolerance development.
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Report
(4 results)
Research Products
(230 results)