Enhanced Angiogenic Potential of Bone Marrow Cells by Hypoxic Preconditioning

• Project/Area Number: 20390345
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Yamaguchi University
• Principal Investigator: FURUTANI Akira Yamaguchi University, 医学部・附属病院, 学術研究員 (90346552)
• Co-Investigator(Kenkyū-buntansha): HAMANO Kimikazu 山口大学, 大学院・医学系研究科, 教授 (60263787) ; KUBO Masayuki 山口大学, 大学院・医学系研究科, 助教 (60420519) ; LI Taoーsheng 山口大学, 大学院・医学系研究科, 講師 (50379997)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 骨髄細胞 / 血管再生 / 低酸素 / プレコンディショニング / 再生医療 / 虚血性疾患 / 加齢 / 糖尿病

Research Abstract

Previous studies have demonstrated that hypoxic preconditioning of bone marrow cells can enhance their potency for inducing therapeutic angiogenesis. However, the exact mechanism of hypoxic preconditioning is not fully understood. Furthermore, experiments have used cells from young healthy individuals. This study shows that hypoxic preconditioning increases the retention of bone marrow cells after implantation into ischemic tissue, and that it also enhances the angiogenic potential of the cells with aged or diabetes-related functional impairment.

Research Products

• [Journal Article] Impaired angiogenic potency of bone marrow cells from patients with advanced age, anemia, and renal failure. (2010)
  • Author(s): Li TS, Kubo M, Ueda K, Murakami M, Mikamo A, Hamano K.
  • Journal Title: J Thorac Cardiovasc Surg. 139(2) Pages: 459-465
  • Peer Reviewed
• [Journal Article] Impaired angiogenic potency of bone marrow cells from patients with advanced age, anemia, and renal failure (2010)
  • Author(s): Tao-Sheng Li
  • Journal Title: The Journal of Thoracic and Cardiovascular Surgery Volume: 139(2) Pages: 459-465
  • Peer Reviewed
• [Journal Article] Increased expression of CXCR4 and integrin ・M in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency. (2009)
  • Author(s): Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K.
  • Journal Title: J Cell Physiol. 220(2) Pages: 508-514
  • Peer Reviewed
• [Journal Article] Antioxidant therapy attenuates diabetes-related impairment of bone marrow stem cells. (2009)
  • Author(s): Ohshima M, Li TS, Kubo M, Qin SL, Hamano K.
  • Journal Title: Circ J. 73(1) Pages: 162-166
  • NAID: 110007007968
  • Peer Reviewed
• [Journal Article] Increased expression of CXCR4 and integrin αM in hypoxia-preconditionde cells contributes to improved cell retention and angiogenic potency (2009)
  • Author(s): Masayuki Kubo
  • Journal Title: Journal of Cellular Physiology 220 Pages: 508-514
  • Peer Reviewed
• [Journal Article] identification of risk factors related to poor angiogenic potency of bone marrow cells from different patients (2009)
  • Author(s): Tao-Sheng Li
  • Journal Title: Circulation 120
  • Peer Reviewed
• [Journal Article] Increased expression of CXCR4 and integrin αM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency (2009)
  • Author(s): Masayuki Kubo, et al
  • Journal Title: Journal of Cellular Physiology (in press)
  • Peer Reviewed
• [Journal Article] Antioxidant therapy attenuates diabetes-related impairment of bone marrow stem cells (2009)
  • Author(s): Mako Ohshima, et al
  • Journal Title: Circulation Journal 73 Pages: 162-166
  • Peer Reviewed
• [Journal Article] Transient increase of cytokines in the acute ischemic tissue is beneficial to cell-based therapeutic angiogenesis. (2008)
  • Author(s): Qin SL, Li TS, Kubo M, Ohshima M, Furutani A, Hamano K.
  • Journal Title: Circ J. 72(12) Pages: 2075-2080
  • NAID: 110006987367
  • Peer Reviewed
• [Journal Article] Hypoxic preconditioning increases survival and angiogenic potency of peripheral blood mononuclear cells via oxidative stress resistance. (2008)
  • Author(s): Kubo M, Li TS, Suzuki R, Shirasawa B, Morikage N, Ohshima M, Qin SL, Hamano K.
  • Journal Title: Am J Physiol Heart Circ Physiol. 294(2)
  • Peer Reviewed
• [Journal Article] Hypoxic preconditioning increases survival and angiogenic potency of peripheral blood mononuclear cells via oxidative stress resistance (2008)
  • Author(s): Masayuki Kubo, et al
  • Journal Title: American Journal of Physiology- Heart and Circulatory Physiology 294
  • Peer Reviewed
• [Presentation] 加齢動物の骨髄細胞に対する低酸素プレコンディショニングの有用性:細胞移植治療の効果向上を目指して (2010)
  • Author(s): 久保正幸, 李桃生, 大島真子, 桂春作, 古谷彰, 濱野公一
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島,広島国際会議場
  • Year and Date: 2010-03-18
• [Presentation] 加齢動物の骨髄細胞に対する低酸素プレコンディショニングの有用性:細胞移植治療の効果向上を目指して (2010)
  • Author(s): 久保正幸
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] Hypoxic pretreatment improves age-related functional imparment of bone marrow cells for inducing therapeutic angiogenesis (2009)
  • Author(s): 久保正幸, 李桃生, 大島真子, 桂春作, 古谷彰, 濱野公一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜,パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Hypoxic pretreatment improves age-related functional impairment of bone marrow cells for inducing therapeutic angiogenesis (2009)
  • Author(s): 久保正幸
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 加齢や糖尿病マウスの骨髄細胞に対する低酸素プレコンディショニングの効果 (2009)
  • Author(s): 大島真子, 李桃生, 久保正幸, 古谷彰, 濱野公一
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京,東京国際フォーラム
  • Year and Date: 2009-03-06
• [Presentation] 加齢や糖尿病マウスの骨髄細胞に対する低酸素プレコンディショニングの効果 (2009)
  • Author(s): 大島 真子
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-06
• [Presentation] 移植細胞の生着率向上に基づく血管新生治療法の開発:低酸素プレコンディショニングの有用性 (2009)
  • Author(s): 久保正幸, 李桃生, 鴨田隆弘, 大島真子, 秦淑蘭, 濱野公一
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京,東京国際フォーラム
  • Year and Date: 2009-03-05
• [Presentation] 移植細胞の生着率向上に基づく血管新生治療法の開発 : 低酸素プレコンディショニングの有用性 (2009)
  • Author(s): 久保 正幸
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] Hypoxic preconditioning of mononuclear cells enhances angiogenesis by increasing their retention after implantation into ischemic tissue (2008)
  • Author(s): 久保正幸, 李桃生, 鴨田隆弘, 大島真子, 秦淑蘭, 濱野公一
  • Organizer: American Heart Association 2008 Scientific Sessions
  • Place of Presentation: New Orleans, Ernest N.Morial Convention Center, USA
  • Year and Date: 2008-11-11
• [Presentation] Hypoxic preconditioning of mononuclear cells enhances angiogenesis by increasing their retention after implantation into ischemic tissue (2008)
  • Author(s): Masayuki Kubo
  • Organizer: American Heart Association Scientific Sessions 2008
  • Place of Presentation: New Orleans (U. S. A. )
  • Year and Date: 2008-11-11
• [Book] 虚血性心疾患に対する心血管再生治療の現状と未来(「びまん性冠動脈病変の臨床-基本的病態理解から治療の選択・実際」) (2010)
  • Author(s): 久保正幸, 李桃生, 濱野公一
  • Publisher: メジカルビュー社
• [Book] 虚血性心疾患に対する心血管再生治療の現状と未来「びまん性冠動脈病変の臨床-基本的病態理解から治療の選択・実際」 (2010)
  • Author(s): 久保正幸
  • Publisher: メジカルビュー社
• [Remarks] ホームページ等 なし