| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2008: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Research Abstract |
The results of the present research project are summarized : 1) The supCD28mAb can preferentially expand nTregs, which induced a potent inhibition of the lethality of GvHD via an antigen-specific manner. These data also provide evidence that GvHD is associated with a decrease of Foxp3 nTregs in the periphery of the host. The determination of the Foxp3 Tregs can be a helpful tool to discriminate the severity and lethality of GvHD after allogeneic stem cell transplantation ; 2) Our rat model showed differences in the suppression of lymphocyte proliferation and GvHD prevention between the short-term cultured conventional BM-MSCs and cloned MSCs and the mechanisms of functional MSCs still remain to be identified ; 3) We demonstrated that utilizing the multiplex PCR GeXP system, to identify a set of immunoregulatory genes which provides sensitivity and specificity for diagnosing rejection and tolerance in mouse hepatic and cardiac transplant models. These data may usher in an era of increased utilization of these gene-based tests for the routine diagnosis of rejection and/or tolerance and thus may be a useful new technology for identification of new biomarkers for the diagnosis of allograft rejection and tolerance in transplant patients.
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