Establishment of a new method of inducing differentiation from human embryonic stem cells to hepatocytes in vitro and investigation of the clinical utility for cell transplantation.
| Project/Area Number |
20390356
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
IKAI Iwao Kyoto University, 医学研究科, 非常勤講師 (60263084)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YASUCHIKA Kentaro 京都大学, 医学研究科, 病院特定助教 (00378895)
ISHII Takamichi 京都大学, 医学研究科, 医員 (70456789)
上本 伸二 京都大学, 医学研究科, 教授 (40252449)
加茂 直子 京都大学, 医学研究科, 医員 (50452355)
波多野 悦朗 京都大学, 医学研究科, 助教 (80359801)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Keywords | ヒトES細胞 / 分化誘導 / 肝細胞 / 細胞移植 / ヒトiPS細胞 |
|---|
| Research Abstract |
We demonstrated that human embryonic stem cells (hESCs) matured into hepatocyte-like cells with higher hepatocyte function in vitro via co-culturing with the fetal murine hepatic cells after administration of hepatocyte growth factor (HGF) and ActivinA. Furthermore, we also demonstrated that human induced pluripotent stem cells (hiPSCs) could be differentiated into hepatocyte-like cells. Moreover, we suggested that transplantation of mature hepatocytes for acute liver failure significantly improved the survival rate in comparison to that of transplantation of immature cells.
|
Report
(4 results)
Research Products
(14 results)
