Clinical application of OMICS for isolation of novel marker in colorectal cancer

• Project/Area Number: 20390357
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: TAKEMASA Ichiro Osaka University, 医学系研究科, 助教 (50379252)
• Co-Investigator(Kenkyū-buntansha): SEKIMOTO Mitugu 大阪大学, 医学系研究科, 准教授 (10273658) ; YAMAMOTO Hirofumi 大阪大学, 医学系研究科, 講師 (30322184) ; IKEDA Masataka 大阪大学, 医学系研究科, 助教 (80335356)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 大腸がん / 個別医療 / OMICS / 大規模症例登録 / 臨床応用 / ファンクショナルゲノミクス / プロテオミクス / メタボロミクス / バイオマーカー

Research Abstract

It has been expected that the personalized medicine is essential for the near future treatment strategy of colorectal cancer with clinical application of the OMICS technology such as functional genomics, proteomics and metabolomics. In this study, several biological markers for the colorectal cancer were successfully isolated in each OMICS level. They are promising and paly important roles for the diagnosis or detection of cancer, the prediction of recurrence or treatment responsiveness that had been difficult with the conventional pathological diagnosis.

Research Products

• [Journal Article] Heterogeneity of colorectal cancers and extraction of discriminator gene signatures for personalized prediction of prognosis. (2011)
  • Author(s): Miyake M, Takemasa I, Matoba R, Tanino M, Niijima S, Ikeda M, Yamamoto H, Sekimoto M, Kuhara S, Okayama T, Monden M, Matsu bara K, Mori M.
  • Journal Title: Int J Clin Oncol. (Epub ahead of print)
• [Journal Article] The Significance of PITX2 Overexpression in Human Colorectal Cancer. (2011)
  • Author(s): Hirose H, Ishii H, Mimori K, Tanaka F, Takemaa I Mizushima T, Ikeda M,Yamamoto H, Sekimoto M,, Doki Y, Mori M.
  • Journal Title: Ann Surg Oncol. (Epub ahead of print)
• [Journal Article] Hypoxia-inducible adrenomedullin in colorectal cancer. (2011)
  • Author(s): Uemura M, Yamamoto H, Takemasa I, Mimori K, Mizushima T, Ikeda M, Sekimoto M, Dok Y,Mori M.
  • Journal Title: Anticancer Res. 2 Pages: 507-514
• [Journal Article] Clinical significance of circulating galectins as colorectal cancer markers. (2011)
  • Author(s): Watanabe M, Takemasa I, Kaneko N, Yokoyama Y, Matsuo E, Iwasa S, Mori M, Matsuura N, Monden M, Nishimura O.
  • Journal Title: Oncol Rep. 25(5) Pages: 1217-1226
• [Journal Article] Significance of Lgr5(+ve)cancer stem cells in the colon and rectum. (2011)
  • Author(s): Takahashi H, Ishii H, Nishida N, Takemasa I, Mizushima T, Ikeda M, Yokobori T, Mimori K, Yamamoto H, Sekimoo M, Dok Y, Mori M.
  • Journal Title: Ann Surg Oncol. 18(4) Pages: 1166-1174
• [Journal Article] Reduction of novel circulating long-chain fatty acids in colorectal cancer patients is independent of tumor burden and correlates with age. (2010)
  • Author(s): Ritchie SA, Heath D, Yamazaki Y, Grimmalt B, Kavianpour A, Krenitsky K, Elshoni H, Takemasa I, Miyake M, Sekimoto M, Monden M, Tomonaga T, Matsubara H, Sogawa K, Matsushita K, Nomura F, Goodenowe DB.
  • Journal Title: BMC Gastroenterol. 10 Pages: 140-140
• [Journal Article] Expression of C4. 4A at the invasive front is a novel prognostic marker for disease recurrence of colorectal cancer. (2010)
  • Author(s): Konishi K, Yamamoto H, Mimori K, Takemasa I, Mizushima T, Ikeda M, Sekimoto M, Matsuura N, Takao T, Doki Y, Mori M.
  • Journal Title: CancerSci. 101(10) Pages: 2269-2277
• [Journal Article] Reduced levels of hydroxylated, polyunsaturated ultra long chain fatty acids in the serum of colorectal cancer patients : implications for early screening an detection. (2010)
  • Author(s): Ritchie SA, Ahiahonu PW, Jayasinghe D, Heath D, Liu J, Lu Y, Jin W, Kavianpour A, Yamazaki Y, Khan AM, Hossain M, Su-Myat KK, Wood PL, Krenitsky K, Takemasa I, Miyake M, Sekimoto M, Monden M, Matsubara H, Nomura F, Goodenowe DB.
  • Journal Title: BMC Med. 15 Pages: 8-13
• [Journal Article] Reduced levels of hydroxylated, polyunsaturated ultra long-chain fatty acids in the serum of colorectal cancer patients : implications for early screening and detection. (2010)
  • Author(s): Ritchie SA, et al
  • Journal Title: BMC Med. Volume: 8(1):13(Epub)
  • Peer Reviewed
• [Journal Article] Reduced levels of hydroxylated, polyunsaturated ultra long-chain fatty acids in the serum of colorectal cancer patients : implications for early screening and detection. (2010)
  • Author(s): Ritchie SA, et al
  • Journal Title: BMC Med. 8(1) : 13(Epub)
  • Peer Reviewed
• [Journal Article] The feasibity of usingiopsy sales from esophagl ccer for comprehensive gene expression profiling. (2009)
  • Author(s): Motoori M, Takemasa I, Yamaski M, Komori T, Takeno A, Miyata H, Takiguchi S, Fujiwara Y, Yasuda T, YanoM, Matsuura N, Matsubara K, Monden M, Mori M, Doki Y.
  • Journal Title: Ann Surg Oncol. 16(7) Pages: 2058-2064
• [Journal Article] マイクロアレイで大腸癌の肝転移予測ができるか教えてください (2009)
  • Author(s): 竹政伊知朗、森正樹
  • Journal Title: Surgery Frontier (4) Pages: 106-109
• [Journal Article] The feasibility of using biopsy samples from esophageal cancer for comprehensive gene expression profiling. (2009)
  • Author(s): Motoori M., et al
  • Journal Title: Ann Surg Oncol. 16(7) : Pages: 2058-2064
  • Peer Reviewed
• [Journal Article] マイクロアレイで大腸癌の肝転移予測ができるか教えてください (2009)
  • Author(s): 竹政伊知朗, 他
  • Journal Title: Surgery Frontier 16(4) : Pages: 106-109
  • Peer Reviewed
• [Journal Article] Gene expression of colorectal cancer : Preoperative genetic diagnosis usinscopic biopsies. (2008)
  • Author(s): Komori T., Takemasa I., Yamasaki M., Motoori M., Kato T., Kikkawa N., Kawaguchi N., Ikeda M., Yamamoto H., Semoto M., Matsubara K., Matsuura N., onden M.
  • Journal Title: Int J Oncol. 32(2) Pages: 367-375
• [Journal Article] Aberrant Expression of Connexin 26 Is Associated with Lung Metastasis of Colorectal Cancer. (2008)
  • Author(s): Ezumi K.,Yammoto H., Murata K., Higashiyama M., Damdinsuren B., Nakamura Y., Kyo N., Okami J., Ngan CY., Takemasa I., Ikeda M., Skimoto M., Matsuura N., Nojima H., Monden M.
  • Journal Title: Clin Cancer Res. 14(3) Pages: 677-684
• [Journal Article] An application of the 2-nitrobenznesulfenyl method to proteomic profiling of human colorectalrcinoA novel approach for biomarker discovery. (2008)
  • Author(s): Watanabe M., M.,Takemasa I., Kawaguchi N., Miyake M., Nishimura N., Matsubara T., Matsuo E., Sekimoto M., Nagai K., Matsuura N., Monden Mishimura O.
  • Journal Title: Proteomics Clin Appl. 2 Pages: 925-935
• [Journal Article] 大腸癌の個別化医療に向けて (2008)
  • Author(s): 竹政伊知朗、池田正孝、山本浩文、関本貢嗣、森正樹、門田守人
  • Journal Title: Frontiers in Gastroenterology. 13(3) Pages: 224-231
• [Journal Article] Gene expression of colorectal cancer : Preoperative genetic diagnosis using endoscopic biopsies. (2008)
  • Author(s): Komori T., Takemasa I, Yamasaki M., Motoori M., Kato T., Kikkawa N., Kawaguchi N., Ikeda M., Yamamoto H., SekimotoM., Matsubara K., Matsuura N., Monden M.
  • Journal Title: Int J Oncol. 32(2) Pages: 367-375
  • Peer Reviewed
• [Journal Article] Aberrant Expression of Connexin 26 Is Associated with Lung Metastasis of Colorectal Cancer. (2008)
  • Author(s): Ezumi K., Yamamoto H., Murata K., Higashiyama M., Damdinsuren B., Nakamura Y., Kyo N., Okami J., Ngan Cy., Takemasa I., Ikcda M., Sekimoto M., Matsuura N., Nojima H., Monden M.
  • Journal Title: Clin Cancer Res. 14(3) Pages: 677-684
  • Peer Reviewed
• [Journal Article] An application of the 2-nitrobenzenesulfenyl method to proteomic profiling of human colorectal carcinoma : A novel approach for biomarker discovery. (2008)
  • Author(s): Watanabe M., Takemasa I., Kawaguchi N., Miyake M., Nishimura N., Matsubara T., MatsuoE., Sekimoto M., Nagai K., Matsuura N., Monden M., Nishimura O.
  • Journal Title: Proteomics Clin Appl. 2 Pages: 925-935
  • Peer Reviewed
• [Journal Article] 転移因手を標的とした肝転移の治療戦略 (2008)
  • Author(s): 山本浩文, 竹政伊知朗, 池田正孝, 関本貢嗣, 門田守人
  • Journal Title: Biotherapy 22(2) Pages: 96-104
• [Journal Article] 大腸癌の個別化医療に向けて (2008)
  • Author(s): 竹政伊知朗, 池田正孝, 山本活文, 関本貢嗣, 森正樹, 門田守人
  • Journal Title: Frontiers in Gastroenterology 13(3) Pages: 224-231
• [Journal Article] 転移因子を標的とした肝転移の治療戦略
  • Author(s): 山本浩文、竹政伊知朗、池田正孝、関本貢嗣、門田守人
  • Journal Title: Biotherapy 22(2) Pages: 96-104
• [Presentation] 生体内低酸素誘導遺伝子検索モデルとしての大腸癌肝転移巣の有用性 (2009)
  • Author(s): 植村守、山本浩文、原口直紹、竹政伊知朗、水島恒和、池田正孝、石井秀始、関本貢嗣、土岐祐一郎、森正樹
  • Organizer: 第20回日本消化器癌発生学会総会
  • Place of Presentation: 広島
• [Presentation] 大腸癌網羅的遺伝子解析を用いた新規マーカー探索:余後関連遺伝子とそのタンパク発現 (2009)
  • Author(s): 三宅正和、竹政伊知朗、渡辺真、池田正孝、山本浩文、水島恒和、関本貢嗣、松浦成昭、西村紀、土岐祐一郎、森正樹
  • Organizer: 第109回日本外科学会期学術集会
  • Place of Presentation: 福岡
• [Presentation] NBS法による大腸癌組織を用いた新規肝転移関連蛋白の検討 (2009)
  • Author(s): 徳岡優佳、竹政伊知朗、渡辺真、三宅正和、水島恒和、池田正孝、山本浩文、関本貢嗣、西村紀、門田守人、森正樹
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] 癌と間質のinterfaceにおける分子解析と治療応用 (2009)
  • Author(s): 山本浩文、小西健、鈴木玲、原口直紹、水島恒和、竹政伊知朗、池田正孝、石井秀始、関本貢嗣、土岐祐一郎、森正樹
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] NBS法を用いた網羅的タンパク解析による大腸癌関連マーカーの探索 (2009)
  • Author(s): 三宅正和、竹政伊知朗、渡辺真、池田正孝、山本浩文、水島恒和、関本貢嗣、松浦成昭、西村紀、土岐祐一郎、森正樹
  • Organizer: 第5回日本消化管学会総会
  • Place of Presentation: 東京
• [Presentation] 癌と間質のinterfaceにおける分子解析と治療応用 (2009)
  • Author(s): 山本浩文, 他
  • Organizer: 第109回 日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] 大腸癌網羅的遺伝子解析を用いた新規マーカー探索:余後関連遺伝子とそのタンパク発現 (2009)
  • Author(s): 三宅正和, 他
  • Organizer: 第109回 日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] NBS法による大腸癌組織を用いた新規肝転移関連蛋白の検討 (2009)
  • Author(s): 徳岡優佳, 他
  • Organizer: 第109回 日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] 生体内低酸素誘導遺伝子検索モデルとしての大膓癌肝転移巣の有用性 (2009)
  • Author(s): 植村守, 他
  • Organizer: 第20回 日本消化器癌発生学会総会
  • Place of Presentation: 広島
• [Presentation] Identificaion of clon cancer-initiating cell. (2008)
  • Author(s): Ishii H.,Haraguchi N., Tanaka F., Mimori K., Inoue H., Takemasa I., Ikda M., Yamamoto H.,ekimoto M.
  • Organizer: 21st the Naito conference.
  • Place of Presentation: 湘南
• [Presentation] NBS法による大腸癌組織を用いた新規転移関連蛋白の検討 (2008)
  • Author(s): 徳岡優佳、竹政伊知朗、渡辺真、三宅正和、人羅俊貴、野村昌哉、池田正孝、山本浩文、関本貢嗣、西村紀、門田守人
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
• [Presentation] 大腸癌網羅的遺伝子解析を用いた新規マーカーを探索:高発現遺伝子とタンパク発現との比較 (2008)
  • Author(s): 三宅正和、竹政伊知朗、池田正孝、山本浩文、関本貢嗣、松原謙一、門田守人
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
• [Presentation] 血管新生因子Adrenomedulinの大腸癌術後予後因子としての意義 (2008)
  • Author(s): 植村守、山本浩文、田中純一、竹政伊知朗、池田正孝、関本貢嗣、門田守人
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
• [Presentation] Identification of colon cancer-imtiatmg cells (2008)
  • Author(s): Ishii H., Haraguchi N., Tanaka F., Mimori K., Inoue H., Takemasa I., Ikeda M., Yamamoto H., Sekimoto M., Mori M.
  • Organizer: 21st the Naito conference
  • Place of Presentation: Shonan
• [Presentation] NBS法による大腸癌組織を用いた新規転移関連蛋白の検討 (2008)
  • Author(s): 徳岡優佳, 竹政伊知朗, 渡辺真, 三宅正和, 人羅俊貴, 野村昌哉, 池田正孝, 山本浩文, 関本貢嗣, 西村紀, 門田守人
  • Organizer: 第108回日本外科字会足期字術集会
  • Place of Presentation: 長崎
• [Presentation] 大腸癌網m勺遺伝子解析を用いた新規マーカーを探索 : 高発現遺伝子とタンパク発現との比較 (2008)
  • Author(s): 三宅正和, 竹政伊知朗, 池田正孝, 山本浩文, 関本貢嗣, 松原謙一, 門田守人
  • Organizer: 第108回日本外科字会足期字術集会
  • Place of Presentation: 長崎
• [Presentation] 皿管新生因子Adrenomedulmの大腸癌術後予後因子としての意義 (2008)
  • Author(s): 植村守, 山本浩文, 田中純一, 竹政伊知朗, 池田正孝, 関本貢嗣, 門田守人
  • Organizer: 第108回日本外科字会足期字術集会
  • Place of Presentation: 長崎
• [Patent(Industrial Property Rights)] 腫瘍マーカー及び癌疾病の罹患の識別方法 (2007)
  • Inventor(s): 渡辺真, 他
  • Industrial Property Rights Holder: 株式会社島津製作所、および国立大学法人大阪大学
  • Filing Date: 2007-06-04