Development of cancer therapy using organelles-targeting nanocarrier

• Project/Area Number: 20390381
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nagoya University
• Principal Investigator: MIZUNO Masaaki Nagoya University, 医学系研究科, 准教授 (70283439)
• Co-Investigator(Kenkyū-buntansha): SHIMATO Shinji 名古屋大学, 大学院・医学系研究科, 助教 (30464142)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 細胞 / ナノテクノロジー / 細胞内小器官 / がん / siRNA

Research Abstract

In this study, we developed nanocarrier for organelles based on the technologies using DEAE polymer and liposome, and succeeded to target a lot of organelles such as mitochondria, chromosome, and contractile ring. When binding siRNA to the nanocarrier, we could confirm he biological activities in in vitro and in vivo experiments. Also, we established the process to produe the siRNA binding nanocarrier under GLP (Good Laboratory Practice) and GMP (Good Manufacturing Practice) control.

Research Products

• [Journal Article] 日本版EHR(Electronic Health Record)構築に向けて (2011)
  • Author(s): 水野正明
  • Journal Title: 電子情報通信学会誌 94 Pages: 172-177
  • NAID: 110008460862
• [Journal Article] A Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier.II. (2011)
  • Author(s): Eshita Y^*, Higashihara J, Onishi M, Mizuno M, Yoshida J, Takasaki T, Yoshioka H, Kubota N, Onishi Y.
  • Journal Title: Its Thixotropy Property. Nanomedicine & Nanotechnology 2 Pages: 1-5
  • Peer Reviewed
• [Journal Article] A Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier. II. Its Thixotropy Property. (2011)
  • Author(s): Eshita Y, et al.
  • Journal Title: Nanomedicine & Nanotechnology Volume: 2 Pages: 1-5
  • Peer Reviewed
• [Journal Article] 日本版EHR(Electronic Health Record)構築に向けて (2011)
  • Author(s): 水野正明
  • Journal Title: 電子情報通信学会誌 Volume: 94 Pages: 172-177
  • NAID: 110008460862
• [Journal Article] Neurosurgical robotic system for brain tumor removal. (2010)
  • Author(s): Arata J^*, Tada Y, Kozuka H, Wada T, Saito Y, Ikedo N, Hayashi Y, Fujii M, Kajita Y, 水野正明, Wakabayashi T, Yoshida J, Fujimoto H.
  • Journal Title: Int J Comput Assist Radiol Surg.
  • Peer Reviewed
• [Journal Article] Development of a real-time tactile sensing system for brain tumor diagnosis. (2010)
  • Author(s): Tanaka Y, Yu Q, Doumoto K, Sano A, Hayashi Y, Fujii M, Kajita Y, 水野正明, Wakabayashi T, Fujimoto H.
  • Journal Title: Int J Comput Assist Radiol Surg. 5(4) Pages: 359-367
  • Peer Reviewed
• [Journal Article] Neurosurgical robotic system for brain tumor removal. (2010)
  • Author(s): Arata J, et al.
  • Journal Title: Int J Comput Assist Radiol Surg.(電子ジャーナルJul 13)
  • Peer Reviewed
• [Journal Article] Development of a real-time tactile sensing system for braintumor diagnosis. (2010)
  • Author(s): Tanaka Y, et al.
  • Journal Title: Int J Comput Assist Radiol Surg. Volume: 5 Pages: 359-67
  • Peer Reviewed
• [Journal Article] Effect of sonoporation on cationic liposome-mediated IFNbeta gene therapy for metastatic hepatic tumors of murine colon cancer. (2009)
  • Author(s): Hayashi S^*, 水野正明, Yoshida J, Nakao A.
  • Journal Title: Cancer Gene Ther 16 Pages: 638-643
  • Peer Reviewed
• [Journal Article] Effect of sonoporation on cationic liposome-mediated IFNbeta gene therapy for metastatic hepatic tumors of murine colon cancer (2009)
  • Author(s): Hayashi S, Mizuno M, Yoshida J, Nakao A
  • Journal Title: Cancer Gene Ther 16 Pages: 638-643
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 水野正明, 吉田純
  • Journal Title: 一方向型地域医療連携におけるICTの利活用(ITvision) Pages: 38-39
• [Journal Article] (2009)
  • Author(s): 水野正明, 吉田純
  • Journal Title: ICTを用いた脳卒中連携医療支機システムの構築(日本医師会雑誌) Pages: 1369-1373
• [Journal Article] Significant antitumor activity of cationic multi- lamellar liposomes containing human interferon- β gene in combination with 5-fluorouracil against human renal cell carcinoma. (2008)
  • Author(s): Yamamoto K, Mizutani Y, Nakanishi H, Fujiwara J, Ishida H, Toiyama D, Abe K, Hayashi I, Okada K, Kawauchi A, 水野正明, Yoshida J, Miki T^*.
  • Journal Title: Int.J Oncol 33 Pages: 565-571
  • Peer Reviewed
• [Journal Article] A phase I clinical trial of interferon- beta gene therapy for high-grade glioma : novel findings from gene expression profiling and autopsy. (2008)
  • Author(s): Wakabayashi T, Natsume A, Hashizume Y, Fujii M, 水野正明, Yoshida J^*.
  • Journal Title: J Gene Med 10 Pages: 329-339
  • Peer Reviewed
• [Journal Article] A pilot study of human interferon beta gene therapy for patients with advanced melanoma by in vivo transduction using cationic liposomes. (2008)
  • Author(s): Matsumoto K, Kubo H, Murata H, Uhara H, Takata M, Shibata S, Yasue S, Sakakibara A, Tomita Y, Kageshita T, Kawakami Y, 水野正明, Yoshida J, Saida T^*.
  • Journal Title: Jpn J Clin Oncol 38 Pages: 849-856
  • Peer Reviewed
• [Journal Article] A phase I clinical trial of interferon-beta gene therapy for high-grade glioma : novel findings from gene expression profiling and autopsy (2008)
  • Author(s): Wakabayashi T, et al.
  • Journal Title: J Gene Med 10 Pages: 329-339
  • Peer Reviewed
• [Journal Article] A pilot study of human interferon beta gene therapy for patients with advanced melanoma by in vivo transduction using cationic liposomes (2008)
  • Author(s): Matsumoto K, et al.
  • Journal Title: Jpn J Clin Oncol 38 Pages: 849-856
  • Peer Reviewed
• [Presentation] インターフェロン遺伝子治療の展開と先端医療開発体制の再整備 (2010)
  • Author(s): 水野正明
  • Organizer: 第48回日本癌治療学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-28
• [Presentation] インターフェロン遺伝子治療の展開と先端医療開発体制の再整備 (2010)
  • Author(s): 水野正明
  • Organizer: 第48回日本癌治療学会
  • Place of Presentation: グランドプリンスホテル京都(京都府)
  • Year and Date: 2010-10-28
• [Presentation] 疾患別医療からみた医療情報の標準化及び共有化:日本版EHRの構築に向けて (2010)
  • Author(s): 水野正明
  • Organizer: 第14回日本医療情報学会春季学術大会
  • Place of Presentation: 高松
  • Year and Date: 2010-05-28
• [Presentation] 疾患別医療からみた医療情報の標準化及び共有化:日本版EHRの構築に向けて (2010)
  • Author(s): 水野正明
  • Organizer: 第14回日本医療情報学会春季学術大会
  • Place of Presentation: サンポートホール高松(香川県)
  • Year and Date: 2010-05-28
• [Presentation] 地域医療情報連携システムの現状と展望 (2009)
  • Author(s): 水野正明、吉田純
  • Organizer: 第29回医療情報学連合大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-25
• [Presentation] 連携医療を支えるセキュリティー (2009)
  • Author(s): 水野正明、吉田純
  • Organizer: 第65回放射線技術学会・総会学術大会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2009-04-18
• [Book] がんに対する遺伝子治療脳腫瘍 (2010)
  • Author(s): 水野正明、若林利彦
  • Total Pages: 8
  • Publisher: 日本臨床
• [Book] がんに対する遺伝子治療脳腫瘍 (2010)
  • Author(s): 水野正明
  • Publisher: 日本臨床
• [Book] ITvision-方向型地域医療連携における (2009)
  • Author(s): 水野正明、吉田純
  • Publisher: ICTの利活用
• [Book] ICTを用いた脳卒中連携医療支援システムの構築 (2009)
  • Author(s): 水野正明、吉口純
  • Publisher: 日本医師会雑誌