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Identification of neural induction domain in stem cells and neuronal regenerative medicine using the peptide derived from the domain

Research Project

Project/Area Number 20390389
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionYokohama City University

Principal Investigator

KANNO Hiroshi  Yokohama City University, 医学部, 准教授 (40244496)

Co-Investigator(Kenkyū-buntansha) SAITO Tomoyuki  横浜市立大学, 医学研究科, 教授 (30170517)
ITO Norihiko  横浜市立大学, 医学部, 助教 (80264654)
MIZUKI Nobuhisa  横浜市立大学, 医学研究科, 教授 (90336579)
DEZAWA Mari  東北大学, 大学院・医学研究科, 教授 (50272323)
味村 俊郎  横浜市立大学, 医学研究科, 客員研究員 (50336583)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords神経再生 / 幹細胞 / 神経分化ペプチド / 脊髄再生 / パーキンソン病 / VHL / 機能性ペプチド / 多能性幹細胞 / 神経再生医療 / 神経幹細胞 / 皮膚由来幹細胞 / 脊髄損傷 / 皮膚幹細胞
Research Abstract

We identified a neural induction domain for somatic stem cells at elonign BC binding site in the VHL protein, and show neuronal differentiation of the cells by transfer of the domain peptide linked to protein transduction domain (PTD). In addition, we show that the domain has the same function for the other somatic stem cells, and that BC-box motifs within SOCS-box proteins also have an ability to induce neuronal differentiation. Furthermore, when the domain peptide-transferred stem cells are grafted into recipient nervous tissue, the grafted cells differentiate to neurons and recovery of the neuronal symptom is recognized. Thus, the neuronal differentiation of pluripotent somatic stem cells is occurred by transfer of the neural induction domain peptide linked to PTD and would contribute to neuronal regenerative therapy.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (30 results)

All 2011 2010 2009 2008

All Journal Article (18 results) (of which Peer Reviewed: 7 results) Presentation (10 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Engrafted VHL peptide-delivered bone marrow stromal cells promote spinal cord repair in rats.2010

    • Author(s)
      Yamazaki Y, Kanno H, Maeda K, Yoshida T, Kobayashi N, Kubo A, Yamaguchi Y, Saito T.
    • Journal Title

      Neuroreport. 21

      Pages: 287-292

    • Related Report
      2010 Final Research Report
  • [Journal Article] Skin-derived precursors differentiate into dopaminergic neuronal cells in the brains of Parkinson's disease model rats.2010

    • Author(s)
      Higashida, T, Jitsuki S, Kubo A, Mitsushima D, Kamiya Y, Kanno H
    • Journal Title

      J Neurosurg 113(3)

      Pages: 648-655

    • Related Report
      2010 Final Research Report
  • [Journal Article] Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration.2010

    • Author(s)
      Matsuse D, Kitada M, Kohama M, Nishikawa K, Makinoshima H, Wakao S, Fujiyoshi Y, Heike T, Nakahata T, Akutsu H, Umezawa A, Harigae H, Kira J, Dezawa M.
    • Journal Title

      Neuropathol Exp Neurol. 69(9)

      Pages: 973-985

    • NAID

      10030408600

    • Related Report
      2010 Final Research Report
  • [Journal Article] Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration.2010

    • Author(s)
      Wakao S, Hayashi T, Kitada M, Kohama M, Matsue D, Teramoto N, Ose T, Itokazu Y, Koshino K, Watabe H, Iida H, Takamoto T, Tabata Y, Dezawa M.
    • Journal Title

      Exp Neurol. 223(2)

      Pages: 537-547

    • Related Report
      2010 Final Research Report
  • [Journal Article] Unique multipotent cells in adult human mesenchymal cell populations.2010

    • Author(s)
      Kuroda Y, Kitada M, Wakao S, Nishikawa K, Tanimura Y, Makinoshima H, Goda M, Akashi H, Inutsuka A, Niwa A, Shigemoto T, Nabeshima Y, Nakahata T, Nabeshima Y, Fujiyoshi Y, Dezawa M.
    • Journal Title

      Proc Natl Acad Sci USA. 107(19)

      Pages: 8639-8643

    • Related Report
      2010 Final Research Report
  • [Journal Article] Skin-derived precursors differentiate into dopaminergic neuronal cells in the brains of Parkinson's disease model rats2010

    • Author(s)
      Higashida, T, Jitsuki S, Kubo A, Mitsushima D, Kamiya Y, Kanno H
    • Journal Title

      Journal of Neurosurgery

      Volume: 113 Pages: 648-655

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Engrafted VHL peptide-delivered bone marrow stromal cells promote spinal cord repair in rats2010

    • Author(s)
      Yamazaki Y, Kanno H, Maeda K, Yoshida T, Kobayashi N, Kubo A, Yamaguchi Y, Saito T.
    • Journal Title

      Neuroreport 21

      Pages: 287-292

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal differentiation of neural progenitor cells by intracellular delivery of synthetic oligopeptide derived from Von Hippel-Lindau protein.2009

    • Author(s)
      Kanno H, Nakano S, Kubo A, Mimura T, Tajima N, Sugimoto N.
    • Journal Title

      Protein Pept Lett. 16

      Pages: 1291-1296

    • Related Report
      2010 Final Research Report
  • [Journal Article] Efficient generation of dopamine neuron-like cells from skin-derived precursors with a synthetic peptide derived from von Hippel-Lindau protein.2009

    • Author(s)
      Kubo A, Yoshida T, Kobayashi N, Yokoyama T, Mimura T, Nishiguchi T, Higashida T, Yamamoto I, Kanno H.
    • Journal Title

      Stem Cells Dev. 18

      Pages: 1523-1532

    • Related Report
      2010 Final Research Report
  • [Journal Article] Transplantation of Von Hippel-Lindau peptide delivered neural stem cells promotes recovery in the injured rat spinal cord.2009

    • Author(s)
      Maeda K, Kanno H, Yamazaki Y, Kubo A, Sato F, Yamaguchi Y, Saito T.
    • Journal Title

      Neuroreport. 20

      Pages: 1559-15563

    • Related Report
      2010 Final Research Report
  • [Journal Article] Committed neural progenitor cells derived from genetically modified bone marrow stromal cells ameliorate deficits in a rat model of stroke.2009

    • Author(s)
      Hayase M, Kitada M, Wakao S, Itokazu Y, Nozaki K, Hashimoto N, Takagi Y, Dezawa M.
    • Journal Title

      J Cereb Blood Flow Metab. 29(8)

      Pages: 1409-1420

    • Related Report
      2010 Final Research Report
  • [Journal Article] Practical induction system for dopamine-producing cells from bone marrow stromal cells using spermine-pullulan-mediated reverse transfection method.2009

    • Author(s)
      Nagane K, Kitada M, Wakao S, Dezawa M, Tabata Y.
    • Journal Title

      Tissue Eng Part A. 15(7)

      Pages: 1655-1665

    • Related Report
      2010 Final Research Report
  • [Journal Article] Neuronal differentiation of neural progenitor cells by intracellular delivery of synthetic oligopeptide derived from von Hippel-Lindau protein2009

    • Author(s)
      Kanno H, Nakano S, Kubo A, Mimura T, Tajima N, Sugimoto N
    • Journal Title

      Protein and Peptide Letters 16

      Pages: 1291-1296

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient generation of dopamine neuron-like cells from skin-derived precursors with a synthetic peptide derived from von Hippel-Lindau protein2009

    • Author(s)
      Kubo A, Yoshida T, Kobayashi N, Yokoyama T, Mimura T, Nishiguchi T, Higashida T, Yamamoto I, Kanno H
    • Journal Title

      Stem Cells and Development 18

      Pages: 1523-1532

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of Von Hippel-Lindau peptide delivered neural stem cells promotes recovery in the injured rat spinal cord2009

    • Author(s)
      Maeda K, Kanno H, Yamazaki Y, Kubo A, Sato F, Yamaguchi Y, Saito T
    • Journal Title

      Neuroreport 20

      Pages: 1559-1563

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脳神経外科領域における再生医療の展望2009

    • Author(s)
      菅野 洋, 川原信隆
    • Journal Title

      Current Insights in Neurological Scienece 17(1)

      Pages: 2-4

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Neuronal differentiation of neural progenitor cells by intracellular delivery of synthetic oligopeptide derived from von Hippel-Lindau protein.2009

    • Author(s)
      Kanno H, Nakano S, Kubo A, Mimura T, Tajima N, Sugimoto N
    • Journal Title

      Protein and Peptide Letters (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient generation of dopamine neuron-like cells from skin-derived precursors with a synthetic peptide derived from von Hippel-Lindau protein.2009

    • Author(s)
      Kubo A, Yoshlda T, Kobayashi N, Yokoyama T, Mimura T, Nishiguchi T, Higashida T, Yamamoto I, Kanno H
    • Journal Title

      Stem Cells and Development (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 多能性体性幹細胞の神経分化を誘導する機能性ペプチドの開発とそれを用いた神経再生医療2010

    • Author(s)
      菅野洋、久保篤彦、東田哲博、 川原信隆
    • Organizer
      第69回日本脳神経外科学会
    • Place of Presentation
      福岡:福岡コンベンションセンター
    • Year and Date
      2010-10-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neuronal differentiation of stem cells by transfer of VHL peptide and regenerative therapy.2010

    • Author(s)
      Kanno H, Kubo A, Higashida T, Kawahara N
    • Organizer
      9^<th> International Medical Symposium on VHL
    • Place of Presentation
      リオデジェネイロ、ブラジル
    • Year and Date
      2010-10-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neuronal differentiation of stem cells by transfer of VHL peptide and regenerative therapy2010

    • Author(s)
      菅野洋, 久保篤彦, 東田哲博, 川原信隆
    • Organizer
      9^<th> International Medical Symposium on VHL
    • Place of Presentation
      リオデジャネイロ
    • Related Report
      2010 Final Research Report
  • [Presentation] 多能性体性幹細胞の神経分化を誘導する機能性ペプチドの開発とそれを用いた神経再生医療2010

    • Author(s)
      菅野洋、久保篤彦、東田哲博、川原信隆
    • Organizer
      第70回日本脳神経外科学会総会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒト成人皮膚よりの多能性幹細胞の分離と機能性VHLペプチド導入による神経分化誘導2010

    • Author(s)
      菅野洋、久保篤彦、川原信隆、三上太郎、前川二郎
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] ヒト成人皮膚よりの多能性幹細胞の分離と機能性 BC-box ペプチド導入による神経分化誘導2009

    • Author(s)
      菅野洋, 久保篤彦, 川原信隆, 三上太郎, 前川二郎
    • Organizer
      第10回日本分子脳神経外科学会
    • Place of Presentation
      岡山
    • Year and Date
      2009-10-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト成人皮膚よりの多能性幹細胞の分離と機能性BC-boxペプチド導入による神経分化誘導2009

    • Author(s)
      菅野洋、久保篤彦、川原信隆、三上太郎、前川二郎
    • Organizer
      第10回日本分子脳神経外科学会
    • Place of Presentation
      岡山(シンポジウム)
    • Related Report
      2010 Final Research Report
  • [Presentation] 組織幹細胞の神経分化を誘導する機能性ペプチドを用いた神経再生療法の開発2008

    • Author(s)
      菅野洋, 久保篤彦, 東田哲博, 西口隆雄, 川原信隆, 小林菜穂子, 増田順一, 吉田徹彦
    • Organizer
      第68回日本脳神経外科学会総会
    • Place of Presentation
      盛岡市
    • Year and Date
      2008-10-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] 機能性BC-boxペプチドによる組織幹細胞の神経細胞への分化誘導とその神経再生医療への応用2008

    • Author(s)
      菅野 洋、久保篤彦、東田哲博、西口隆雄、川原信隆, 増田順一、小林菜穂子、吉田徹彦
    • Organizer
      第9回日本分子脳神経外科学会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 組織幹細胞の神経分化を誘導する機能性ペプチドを用いた神経再生療法の開発2008

    • Author(s)
      菅野洋, 久保篤彦, 東田哲博, 西口隆雄, 川原信隆, 小林菜穂子, 増田順一, 吉田徹彦
    • Organizer
      第68回日本脳神経外科学会総会
    • Place of Presentation
      盛岡
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 神経分化抑制ペプチド及びその利用2011

    • Inventor(s)
      菅野洋, 吉田徹彦, 小林菜穂子
    • Industrial Property Rights Holder
      菅野洋、東亜合成株式会社
    • Acquisition Date
      2011-02-04
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] VHLペプチド2010

    • Inventor(s)
      菅野洋, 吉田徹彦, 小林菜穂子
    • Industrial Property Rights Holder
      菅野洋、東亜合成株式会社
    • Acquisition Date
      2010-04-16
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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