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Disc repair with transplantation of activated nucleus pulposus cells-how to preserve the cells and its safety-

Research Project

Project/Area Number 20390405
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokai University

Principal Investigator

MOCHIDA Joji (2009-2011)  東海大学, 医学部, 教授 (30129697)

持田 譲治 (2008)  Tokai University, 医学部, 教授 (50174347)

Co-Investigator(Kenkyū-buntansha) SAKAI Daisuke  東海大学, 医学部, 講師 (10408007)
IWASHINA Tooru  東海大学, 医学部, 助教 (10433913)
Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords髄核細胞 / 骨髄間葉系幹細胞 / 凍結保存 / 椎間板移植 / 変性抑制 / 椎間板変性 / 活性化髄核細胞 / 凍結保存法 / 安全性
Research Abstract

The purpose of this study is to evaluate the safety and property of the activated nucleus pulposus cells after the cryopreservation. With the animal model and human material, we compared safety and property of the nucleus pulposus cells, ordinarily activated, to those in two subgroups on cryopreservation method ; 1) activated nucleus pulposus cells after cryopreservation for two weeks and 2) nucleus pulposus tissue itself after cryopreservation for two weeks. There was no significant difference amongst those three subgroups on the safety, the proliferation of the nucleus pulposus cells or the potentiality for the cell matrix in both animal and human materials. We confirmed cryopreservation of the nucleus pulposus is useful to repair various disc lesions.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 2010 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (17 results)

  • [Journal Article] Human nucleus pulposus cells significantly enhanced biological properties in a coculture system with direct cell-to-cell contact with autologous mesenchymal stem cells2010

    • Author(s)
      Watanabe T, Sakai D, Yamamoto Y, Iwashina T, Serigano K, Tamura F, Mochida J
    • Journal Title

      Journal of Orthopaedic Research

      Volume: Vol.28 Pages: 623-630

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human nucleus pulposus cells significantly enhanced biological properties in a coculture system with direct cell-to-cell contact with autologous mesenchymal stem cells.2010

    • Author(s)
      Watanabe T, Sakai D, Yamamoto Y, Iwashina T, Serigano K, Tamura F, Mochida J.
    • Journal Title

      Journal of Orthopaedic Research Vol 28

      Pages: 623-630

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] 細胞レベルからの椎間板再生 -細胞移植療法のその先に-2009

    • Author(s)
      酒井大輔、持田譲治
    • Journal Title

      日本腰痛学会雑誌 第15巻

      Pages: 95-98

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential phenotype of intervertebral disc cells : microarray and immunohistochemical analysis of canine nucleus pulposus and anulus fibrosus2009

    • Author(s)
      Sakai D, Nakai T, Mochida J, Alini M, Grad S
    • Journal Title

      Spine 34

      Pages: 1448-1456

    • NAID

      10027244193

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human nucleus pulposus cells significantly enhanced biological properties in a coculture system with direct cell-to-cell contact with autologous mesenchymal stem cells2009

    • Author(s)
      Watanabe T, Sakai D, YamamotoY, Iwashina T, Serigano K, Tamura F, Mochida J
    • Journal Title

      J Orthop Res. 28

      Pages: 623-630

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] stem cell applications in intervertebral discrepair2008

    • Author(s)
      檜山明彦, 持田譲治, 酒井大輔
    • Journal Title

      Cell and Molecular Biology 54

      Pages: 24-32

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Non-notochordal origin in chondrocyte-like cells of the nucleus pulposus appearing in early stage disc degeneration in the mouse model2012

    • Author(s)
      田中真弘、酒井大輔、檜山明彦、田村太、新井文征、中島大輔、中井知子、持田讓治
    • Organizer
      2012 Annual Meeting of Orthopaedic Research Society
    • Place of Presentation
      San Francisco CA, USA
    • Year and Date
      2012-02-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 活性化髄核細胞の凍結保存方法についての基礎的検討22011

    • Author(s)
      田中真弘、酒井大輔、檜山明彦、田村太、新井文征、中島大輔、中井知子、持田讓治
    • Organizer
      第26回日本整形外科学会基礎学術集会
    • Place of Presentation
      ベイシア文化ホール、前橋
    • Year and Date
      2011-10-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 活性化髄核細胞の凍結保存方法についての基礎的検討(第2報)2011

    • Author(s)
      田中真弘, 酒井大輔, 檜山明彦, 新井文征, 中島大輔, 中井知子, 持田讓治
    • Organizer
      日本整形外科学会雑誌
    • Year and Date
      2011-08-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Basic research on cryopreservation of aqctivated human nucleus pulposus cells2011

    • Author(s)
      Tanaka M, Sakai D, Mochida J
    • Organizer
      ISSLS meeting at Gotenburg
    • Place of Presentation
      Sweden
    • Year and Date
      2011-06-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Basic research on cryopreservation of activated human nucleus pulposus cells2011

    • Author(s)
      田中真弘、酒井大輔、檜山明彦、田村太、新井文征、中島大輔、中井知子、持田讓治
    • Organizer
      2011 Annual Meeting of International Society for the Study of the Lumbar Spine
    • Place of Presentation
      Gothenburg, Sweden
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 活性化髄核細胞の凍結保存法についての基礎的検討2010

    • Author(s)
      田中真弘、酒井大輔、新井文征、持田讓治
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2010-10-14
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 椎間板再生のための細胞移植療法臨床研究の進捗および幹細胞研究による新展開2010

    • Author(s)
      酒井大輔、田中真弘、新井文征、持田讓治
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2010-10-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 活性化髄核細胞の凍結保存法についての基礎的検討2010

    • Author(s)
      田中真弘、酒井大輔、新井文征、持田讓治
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都市
    • Year and Date
      2010-10-14
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 椎間板再生のための細胞移植療法 臨床研究の進捗および幹細胞研究による新展開2010

    • Author(s)
      酒井大輔、田中真弘、新井文征、持田讓治
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都市
    • Year and Date
      2010-10-14
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 椎間板再生のための細胞移植療法 臨床研究の進捗および幹細胞研究による新展開2010

    • Author(s)
      酒井大輔、田中真弘、新井文征、持田讓治
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2010-10-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] シンウポジム 骨髄間葉系幹細胞により活性化された髄細胞を用いた椎間板再生医療2009

    • Author(s)
      持田譲治
    • Organizer
      第31回日本バイオマテリアル学会大会
    • Place of Presentation
      京都
    • Year and Date
      2009-11-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 椎間板再生のための細胞移植療法2009

    • Author(s)
      持田譲治
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 椎間板ヘルニアに対する最新医療-橋渡し研究の経過-2009

    • Author(s)
      持田譲治
    • Organizer
      第112回中部日本整形外科災害外科学会・学術集会
    • Place of Presentation
      京都
    • Year and Date
      2009-04-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] シンポジウム 自家骨髄間葉系幹細胞により活性化された椎間板髄核細胞を用いた椎間板再生 実現過程での問題点と対処2009

    • Author(s)
      持田譲治, 酒井大輔
    • Organizer
      第8回日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 細胞移植による椎間板再生医療2008

    • Author(s)
      持田譲治
    • Organizer
      日本腰痛学会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] Trends of spinal basic research in Japan Activation of nucleus pulposus cells by bone marrow-derived Mesenchymal stem cell2008

    • Author(s)
      持田譲治
    • Organizer
      The 7th combined comgress of the spine and pediatric sections of Asia Pacific Orthopaedic Associatio
    • Place of Presentation
      済州島、韓国
    • Related Report
      2008 Annual Research Report
  • [Presentation] 椎間板再生 臨床応用への橋渡し研究の現況2008

    • Author(s)
      持田譲治
    • Organizer
      第37回日本脊椎脊髄病学会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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