Study of TGFβ signaling during angiogenesis in bone metastasis sites by using in vivo optical imaging

• Project/Area Number: 20390407
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Ehime University (2010); Japanese Foundation For Cancer Research (2008-2009)
• Principal Investigator: IMAMURA Takeshi Ehime University, 大学院・医学系研究科, 教授 (70264421)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: バイオテクノロジー / シグナル伝達 / 酵素 / 生体分子 / 細胞・組織 / シグナル云達

Research Abstract

In order to clarify the roles of TGF-β/BMP signaling during angiogenesis in bone metastasis sites of breast cancer, we developed advanced in vivo optical imaging systems by which we can visualize tumor angiogenesis and TGF-β/BMP at the same time. In addition, we studied the effects of angiogenesis inhibitors and TGF-β/BMP signaling blockers during bone metastasis of breast cancer in vivo.

Research Products

• [Journal Article] Arkadia complexes with clathrin adaptor AP2 and regulates EGF signaling (2011)
  • Author(s): Mizutani A, et al.
  • Journal Title: J Biochem. Volume: 148 Pages: 733-41
  • Peer Reviewed
• [Journal Article] Arkadia complexes with clathrin adaptor AP2 and regulates EGF signaling (2010)
  • Author(s): Mizutani A, Saitoh M, Imamura T, Miyazawa K, Miyazono K.
  • Journal Title: J Biochem 148 Pages: 733-741
  • NAID: 10027879992
  • Peer Reviewed
• [Journal Article] Estrogen inhibits transforming growth factor b signaling by promoting Smad2/3 degradation (2010)
  • Author(s): Ito I, Hanyu A, Wayama M, Goto N, Katsuno Y, Kawasaki S, Nakajima Y, Kajiro M, Komatsu Y, Fujimura A, Hirota R, Murayama A, Kimura K, Imamura T, Yanagisawa J.
  • Journal Title: J Biol Chem 285 Pages: 14747-1455
  • Peer Reviewed
• [Journal Article] Runx1 and Runx2 cooperate during sternal morphogenesis (2010)
  • Author(s): Kimura A, Inose H, Yano F, Fujita K, Ikeda T, Sato S, Iwasaki M, Jinno T, Ae K, Fukumoto S, Takeuchi Y, Itoh H, Imamura T, Kawaguchi H, Chung UI, Martin JF, Iseki S, Shinomiya K, Takeda S.
  • Journal Title: Development 137 Pages: 1159-1167
  • Peer Reviewed
• [Journal Article] TGF-b type II Receptor mRNA in Retinoblastoma Cell Lines is induced by Trichostatin A (2010)
  • Author(s): Kashiwagi Y, Horie K, Kanno C, Inomata M, Imamura T, Kato M, Yamamoto T, Yamashita H
  • Journal Title: Invest Ophthalmol Vis Sci 51 Pages: 679-685
  • Peer Reviewed
• [Journal Article] Estrogen inhibits transforming growth factor beta signaling by promoting Smad2/(3)degradation (2010)
  • Author(s): Ito I, et al.
  • Journal Title: J Biol Chem 285 Pages: 14747-55
  • Peer Reviewed
• [Journal Article] Runx1 and Runx2 cooperate during sternal morphogenesis (2010)
  • Author(s): Kimura A, et al.
  • Journal Title: Development. 137 Pages: 1159-67
  • Peer Reviewed
• [Journal Article] Trichostatin A-induced TGF-β type II receptor expression in retinoblastoma cell lines (2010)
  • Author(s): Kashiwagi Y, et al.
  • Journal Title: Invest Ophthalmol Vis Sci. 51 Pages: 679-85
  • Peer Reviewed
• [Journal Article] Promoter-wide analysis of Smad4 binding sites in human epithelial cells (2009)
  • Author(s): Koinuma D, Tsutsumi S, Kamimura N, Imamura T, Aburatani H, Miyazono K
  • Journal Title: Cancer Sci 100 Pages: 2133-2142
  • NAID: 10027197070
  • Peer Reviewed
• [Journal Article] Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells (2009)
  • Author(s): Hanyu A, Kojima K, Hatake K, Nomura K, Murayama H, Ishikawa Y, Miyata S, Ushijima M, Matsuura M, Ogata E, Miyazawa K, Imamura T
  • Journal Title: Cancer Sci 100 Pages: 2085-2092
  • NAID: 10027196865
  • Peer Reviewed
• [Journal Article] Mechanical stress induces Interleukin-11 expression to stimulate osteoblast differentiation (2009)
  • Author(s): Kido S, Kuriwaka-Kido R, Imamura T, Ito Y, Inoue D, Matsumoto T.
  • Journal Title: Bone 45 Pages: 1125-1132
  • Peer Reviewed
• [Journal Article] Role of Ras signaling in the induction of snail by TGF-b (2009)
  • Author(s): Horiguchi K, Shirakihara T, Nakano A, Imamura T, Miyazono K, Saitoh M
  • Journal Title: J Biol Chem 284 Pages: 245-253
  • Peer Reviewed
• [Journal Article] ChIP-chip analysis of Smad2/3 binding sites reveals 1 roles of ETS1 and TFAP2A in TGF-β signaling (2009)
  • Author(s): Koinuma D, Tsutsumi S, Kamimura N, Taniguchi H, Miyazawa K, Sunamura M, Imamura T, Miyazono K, Aburatani H
  • Journal Title: Mol Cell Biol 29 Pages: 172-186
  • Peer Reviewed
• [Journal Article] Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody inxenograft model of human fibrosarcoma HT1080 cells (2009)
  • Author(s): Hanyu A, et al.
  • Journal Title: Cancer Sci. 100 Pages: 2085-92
  • Peer Reviewed
• [Journal Article] Promoter-wide analysis of Smad4 binding sites in human epithelial cells (2009)
  • Author(s): Koinuma D, et al.
  • Journal Title: Cancer Sci. 100 Pages: 2133-42
  • Peer Reviewed
• [Journal Article] Mechanical stress induces lnterleukin-11 expression to stimulate osteoblast differentiation (2009)
  • Author(s): Kido S, et al.
  • Journal Title: Bone. 45 Pages: 1125-32
  • Peer Reviewed
• [Journal Article] Role of Ras signaling in the induction of snail by TGF-β (2009)
  • Author(s): Horiguchi K, et al.
  • Journal Title: J Biol Chem. 284 Pages: 245-53
  • Peer Reviewed
• [Journal Article] ChIP-chip analysis of Smad2/3 binding sites reveals 1 roles of ETS1 and TFAP2A in TGF-β signaling (2009)
  • Author(s): Koinuma D, et al.
  • Journal Title: Mol Cell Biol. 29 Pages: 172-86
  • Peer Reviewed
• [Journal Article] Smurf2 induces ubiquitin-dependent degradation of Smurf1 to prevent migration of breast cancer cells (2008)
  • Author(s): Fukunaga E, Inoue Y, Komiya S, Horiguchi K, Goto K, Saitoh M, Miyazawa K, Koinuma D, Hanyu A, Imamura T
  • Journal Title: J Biol Chem 283 Pages: 35660-35667
  • Peer Reviewed
• [Journal Article] CCAAT/Enhancer-binding Protein b Promotes Osteoblast Differentiation by Enhancing Runx2 Activity with ATF4 (2008)
  • Author(s): Tominaga H, Maeda S, Hayashi M, Takeda S, Akira S, Komiya S, Nakamura T, Akiyama H, Imamura T
  • Journal Title: Mol Biol Cell 19 Pages: 5373-5386
  • Peer Reviewed
• [Journal Article] Regulation of TGF-b family signaling by E3 ubiquitin ligases. (2008)
  • Author(s): Inoue Y, Imamura T
  • Journal Title: Cancer Sci 99 Pages: 2107-2112
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein signaling enhances invasion and bone metastasis of breast cancer cells through Smad pathway (2008)
  • Author(s): Katsuno Y, Hanyu A, Kanda H, Ishikawa Y, Akiyama F, Iwase T, Ogata E, Ehata S, Miyazono K, Imamura T
  • Journal Title: Oncogene. 27 Pages: 6322-6333
  • Peer Reviewed
• [Journal Article] Smurf2 induces ubiquitin-dependent degradation of Smurf1 to prevent migration of breast cancer cells (2008)
  • Author(s): Fukunaga E, et al.
  • Journal Title: J Biol Chem. 283 Pages: 35660-7
  • Peer Reviewed
• [Journal Article] CCAAT/Enhancer-binding Protein β Promotes Osteoblast Differentiation by Enhancing Runx2 Activity with ATF4 (2008)
  • Author(s): Tominaga H, et al.
  • Journal Title: Mol Biol Cell 19 Pages: 5373-86
  • Peer Reviewed
• [Journal Article] Regulation of TGF-β family signaling by E3 ubiquitin ligases (2008)
  • Author(s): Inoue Y, et al.
  • Journal Title: Cancer Sci. 99 Pages: 2107-12
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein signaling enhances invasion and bone metastasis of breast cancer cells through Smad pathway (2008)
  • Author(s): Katsuno Y, et al.
  • Journal Title: Oncogene 27 Pages: 6322-33
  • Peer Reviewed
• [Presentation] 革新的インビボ光イメージングの骨代謝への応用 (2010)
  • Author(s): 今村健志
  • Organizer: 第53回秋季日本歯周病学会学術大会
  • Place of Presentation: かがわ国際会議場(香川県)
• [Presentation] 光技術を駆使した細胞機能イメージングの骨研究への応用 (2010)
  • Author(s): 今村健志
  • Organizer: 第28回日本骨代謝学会学術集会シンポジウム10
  • Place of Presentation: 京王プラザホテル(東京都)
• [Presentation] (2009)
  • Author(s): 今村健志
  • Organizer: 第32回日本分子生物学会年会. Recent progress of in vivo optical imaging of cancer
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-12
• [Presentation] 第32回日本分子生物学会年会 (2009)
  • Author(s): 今村健志
  • Organizer: Recent progress of in vivo optical imaging of cancer
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-12
• [Presentation] 乳がん骨転移におけるTGF-β/BMPシグナルの役割とインビボ光イメージング (2009)
  • Author(s): 今村健志
  • Organizer: 第27回日本骨代謝学会学術集会シンポジウム1
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2009-07-23
• [Presentation] 光イメージングを利用したがん研究新戦略 (2009)
  • Author(s): 今村健志
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉県大宮市
  • Year and Date: 2009-05-16
• [Presentation] がんの血管新生と細胞周期のインビボ光イメージング (2008)
  • Author(s): 今村健志
  • Organizer: 第3回日本分子イメージング学会総会 学術集会
  • Place of Presentation: 埼玉県大宮市0大宮ソニックシティ
  • Year and Date: 2008-05-22
• [Presentation] がんの血管新生と細胞周期のインビボ光イメージング (2008)
  • Author(s): 今村健志
  • Organizer: 第3回日本分子イメージング学会総会・学術集会
  • Place of Presentation: 埼玉県大宮市大宮ソニックシティ
  • Year and Date: 2008-05-22
• [Presentation] 癌の増殖転移における血管新生と骨破壊のインビボイメージング (2008)
  • Author(s): 今村健志
  • Organizer: 第62回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2008-04-17
• [Presentation] 生命のダイナミズム・不均一性・確率性・～個体で細胞を「みて」「知る」～ (2008)
  • Author(s): 今村健志
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市神戸ポートピホテル
• [Presentation] 骨と癌のインビボ光イメージング (2008)
  • Author(s): 今村健志
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪府大阪市大阪国際会議場
• [Presentation] In vivo optical imaging of cancer and microenvironment. (2008)
  • Author(s): 今村健志
  • Organizer: 第2回JCA-AACR合同カンファレンス-乳がん研究の最前線
  • Place of Presentation: 兵庫県淡路市淡路夢舞台国際会議場
• [Presentation] In vivo optical imaging of tumor microenvironment and TGF-β signaling (2008)
  • Author(s): 今村健志
  • Organizer: 第28回札幌がんセミナー国際シンポジウム
  • Place of Presentation: 北海道札幌市北海道大学学術交流会館講堂
• [Presentation] In vivo optical imaging of cancer and microenvironment (2008)
  • Author(s): Takeshi Imamura
  • Organizer: 第2回JCA-AACR合同カンファレンス-乳がん研究の最前線
  • Place of Presentation: 兵庫県淡路市淡路夢舞台国際会議場
• [Presentation] 生命のダイナミズム・不均一性・確率性・〜個体で細胞を「みて」「知る」〜 (2008)
  • Author(s): 今村健志
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市神戸ポートピホテル
• [Remarks] ホームページ等
  • URL: http://www.m.ehime-u.ac.jp/medical/course/ProgramForMedicalSciences/