Non-coding RNAs regulating cartilage development

• Project/Area Number: 20390409
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: ASAHARA Hiroshi National Research Institute for Child Health and Development, システム発生・再生医学研究部, 部長 (70294460)
• Co-Investigator(Kenkyū-buntansha): MIYAKI Shigeru 独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 研究員 (10392490)
• Research Collaborator: KUDO Hiroe 独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 共同研究員 (20425713) ; SATO Tempei 独立行政法人国立成育医療研究センター, システム発生・再生医学研究部, 共同研究員 (00571944)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 関節病学 / 軟骨 / マイクロRNA

Research Abstract

We examined the expression pattern of miRNAs in chondrogenesis and found that miR-140 is specifically expressed in cartilage. Loss and gain of function experiments of miR-140 indicated that miR-140 is critical for both cartilage development and homeostasis, partly via regulating Adamts-5.

Research Products

• [Journal Article] MicroRNA-140 plays dual roles in both cartilage development and homeostasis. (2010)
  • Author(s): Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, Takada S, Lotz MK, Ueno-Kudo H, Asahara H.
  • Journal Title: Genes Dev. 24(11) Pages: 1173-1185
  • Peer Reviewed
• [Journal Article] MicroRNA-140 plays dual roles both in cartilage development and homeostasis (2010)
  • Author(s): Miyaki S, et al.
  • Journal Title: Genes & Development Volume: 24(11) Pages: 1173-1185
  • Peer Reviewed
• [Journal Article] Real-time functional imaging for monitoring miR-133 during myogenic differentiation. (2009)
  • Author(s): Kato Y, Miyaki S, Yokoyama S, Omori S, Inoue A, Horiuchi M, Asahara H.
  • Journal Title: Int J Biochem Cell Biol. 41(11) Pages: 2225-2231
  • Peer Reviewed
• [Journal Article] MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates IL-1 responses. (2009)
  • Author(s): Miyaki S, Nakasa T, Otsuki S, Grogan SP, Higashiyama R, Inoue A, Kato Y, Sato T, Lotz MK, Asahara H.
  • Journal Title: Arthritis Rhuem. 60(9) Pages: 2723-2730
  • Peer Reviewed
• [Journal Article] Sox9 directily promotes Bapx1 gene expression to repress Runx2 in chondrocytes. (2009)
  • Author(s): Yamashita S, Andoh M, Ueno-Kudoh H, Sato T, Miyaki S, Asahara H.
  • Journal Title: Exp Cell Res. 315(13) Pages: 2231-2240
  • Peer Reviewed
• [Journal Article] Smad3 activates the Sox9-dependent transcription on chromatin. (2009)
  • Author(s): Furumatsu T, Ozaki T, Asahara H.
  • Journal Title: Int J Biochem Cell Biol. 41(5) Pages: 489-497
  • NAID: 120003338815
  • Peer Reviewed
• [Journal Article] MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates IL-1 responses (2009)
  • Author(s): Miyaki S, et al.
  • Journal Title: Arthritis and rheumatism 60(9) Pages: 2723-2730
  • Peer Reviewed
• [Journal Article] Sox9 directly promotes Bapx1 gene expression to repress Runx2 in chondrocytes (2009)
  • Author(s): Yamashita S, et al.
  • Journal Title: Exp Cell Res (in press)
  • Peer Reviewed
• [Journal Article] Real-time functional imaging for monitoring miR-133 during myogenic differentiation (2009)
  • Author(s): Kato Y, et al.
  • Journal Title: Int J Biochem Cell Biol (in press)
  • Peer Reviewed
• [Journal Article] MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates IL-1 responses (2009)
  • Author(s): Miyaki S, et al.
  • Journal Title: Arthritis Rheum (in press)
  • Peer Reviewed
• [Journal Article] Expression of microRNA-146 in rheumatoid arthritis synovial tissue. (2008)
  • Author(s): Nakasa T, Miyaki S, Okubo A, Hashimoto M, Nishida K, Ochi M, Asahara H.
  • Journal Title: Arthritis Rheum. 58(5) Pages: 1284-1292
  • Peer Reviewed
• [Journal Article] Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken. (2008)
  • Author(s): Yokoyama S, Hashimoto M, Ahimizu H, Ueno-Kudoh H, Uchibe K, Kimura I, Asahara H.
  • Journal Title: Gene Expr Patterns. 8(3) Pages: 155-160
  • Peer Reviewed
• [Journal Article] Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken (2008)
  • Author(s): Yokoyama S, et al.
  • Journal Title: Gene Expr Patterns 8(3) Pages: 155-160
  • Peer Reviewed
• [Journal Article] Expression of microRNA-146 in rheumatoid arthritis synovial tissue (2008)
  • Author(s): Nakasa T, et al.
  • Journal Title: Arthritis Rheum 58(5) Pages: 1284-1292
  • Peer Reviewed
• [Journal Article] Smad3 activates the Sox9-dependent transcription on chromatin (2008)
  • Author(s): Furumatsu T, et al.
  • Journal Title: Int J Biochem Cell Biol 41(5) Pages: 489-497
  • Peer Reviewed
• [Journal Article] MicroRNA-140 plays dual roles both in cartilage development and homeostasis
  • Author(s): Miyaki S, et al.
  • Journal Title: Genes & development (印刷中)
  • Peer Reviewed
• [Presentation] Transcription and RNA network regulating locomotive system development and homeostasis. (2011)
  • Author(s): 浅原弘嗣
  • Organizer: Gordon Conferences -Cartilage Biology & Pathology-.
  • Place of Presentation: Ventura Beach Marriott(米国)
  • Year and Date: 2011-03-07
• [Presentation] Transcription and RNA network regulating locomotive system development and homeostasis (2011)
  • Author(s): Asahara H
  • Organizer: Cordon Conferences-Cartilage Biology & Pathology
  • Place of Presentation: Ventura Beach Marriott(米国)
  • Year and Date: 2011-03-07
• [Presentation] システムアプローチによる運動器発生メカニズムと疾患の解明 (2011)
  • Author(s): 浅原弘嗣
  • Organizer: 科学研究費補助金 基盤研究S「内軟骨性骨形成過程における転写制御ネットワークシステムの統合的理解」公開シンポジウム
  • Place of Presentation: 千里阪急ホテル(大阪府)
  • Year and Date: 2011-01-21
• [Presentation] システムアプローチによる運動器発生メカニズムと疾患の解明 (2011)
  • Author(s): 浅原弘嗣
  • Organizer: 科学研究費補助金基盤研究S「内軟骨性骨形成過程における転写制御ネットワークシステムの統合的理解」公開シンポジウム
  • Place of Presentation: 千里阪急ホテル(大阪府)
  • Year and Date: 2011-01-21
• [Presentation] システムアプローチによる運動器の発生と疾患の解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] システムアプローチによる運動器の発生と疾患の解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] システム研究による運動器の発生メカニズムの解明と医療への応用 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第51回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 秋葉原コンベンションホール(東京都)
  • Year and Date: 2010-09-05
• [Presentation] システム研究による運動器の発生メカニズムの解明と医療への応用 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第51回日本組織細胞化学会総会学術集会
  • Place of Presentation: 秋葉原コンベンションホール(東京都)
  • Year and Date: 2010-09-05
• [Presentation] システムアプローチによる運動器の発生と疾患の解析 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第19回硬組織再生生物学会学術大会
  • Place of Presentation: 就実大学E館(教育実践センター)(岡山県)
  • Year and Date: 2010-09-04
• [Presentation] システムアプローチによる運動器疾患の解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 日本ヒトプロテオーム機構第8回大会(日本プロテオーム学会2010年会) 第6回日本臨床プロテオーム研究会
  • Place of Presentation: 東京ベイホテル東急(千葉県)
  • Year and Date: 2010-07-27
• [Presentation] システムアプローチによる運動器疾患の解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 日本ヒトプロテオーム機構第8回大会(日本プロテオーム学会2010年会)第6回日本臨床プロテオーム研究会
  • Place of Presentation: 東京ベイホテル東急(千葉県)
  • Year and Date: 2010-07-27
• [Presentation] A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 2^<nd> Joint Meeting of the French and Japanese Societies for Developmental Biology.
  • Place of Presentation: Institut Pasteur(フランス)
  • Year and Date: 2010-05-27
• [Presentation] A systems approach reveals that the myogeneais genome network is regulated by the transcriptional repressor RP58 (2010)
  • Author(s): Asahara H
  • Organizer: 2^<nd> Joint Meeting of the French and Japanese Societies for Developmental Biology
  • Place of Presentation: Institut Pasteur(フランス)
  • Year and Date: 2010-05-27
• [Presentation] システムアプローチによる運動器発生メカニズムの解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第23回日本軟骨代謝学会
  • Place of Presentation: 鹿児島県医師会館(鹿児島県)
  • Year and Date: 2010-04-03
• [Presentation] システムアプローチによる運動器の分化制御機構の解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第3回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2010-03-19
• [Presentation] システムアプローチによる運動器の分化制御機構の解明 (2010)
  • Author(s): 浅原弘嗣
  • Organizer: 第3回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場(広島県広島市)
  • Year and Date: 2010-03-19
• [Presentation] Systems approach reveals transcriptional network regulated by RP58. (2009)
  • Author(s): 浅原弘嗣
  • Organizer: International Joint Syposium on "Cell Fate Regulation Research : Stem cells and organogenesis".
  • Place of Presentation: 熊本大学発生医学研究所(熊本県)
  • Year and Date: 2009-11-26
• [Presentation] Systems approach reveals transcriptional network regulated by RP58 (2009)
  • Author(s): 浅原弘嗣
  • Organizer: International Joint Syposium on "Cell Fate Regulation Research : Stem cells and organogenesis"
  • Place of Presentation: 熊本大学発生医学研究所(熊本県熊本市)
  • Year and Date: 2009-11-26
• [Presentation] miRNAによる軟骨代謝制御メカニズム (2009)
  • Author(s): 浅原弘嗣
  • Organizer: 第1回Orthopedic Research Club.
  • Place of Presentation: かずさアカデミアパーク(千葉県)
  • Year and Date: 2009-11-01
• [Presentation] miRNAによる軟骨代謝制御メカニズム (2009)
  • Author(s): 浅原弘嗣
  • Organizer: 第1回 Orthopedic Research Club
  • Place of Presentation: かずさアカデミアパーク(千葉県木更津市)
  • Year and Date: 2009-11-01
• [Presentation] システムアプローチによる筋、軟骨、腱の分化制御機構の解明 (2009)
  • Author(s): 伊藤義晃, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-21
• [Presentation] システムアプローチによる筋、軟骨、腱の分化制御機構の解明 (2009)
  • Author(s): 伊藤義晃, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県神戸市)
  • Year and Date: 2009-10-21
• [Presentation] 関節リウマチに患者におけるmicroRNA (2009)
  • Author(s): 浅原弘嗣
  • Organizer: 第2回基礎と臨床を結ぶ分子病態研究会
  • Place of Presentation: 学士会館(東京都)
  • Year and Date: 2009-10-10
• [Presentation] 関節リウマチに患者におけるmicroRNA (2009)
  • Author(s): 浅原弘嗣
  • Organizer: 第2回基礎と臨床を結ぶ分子病態研究会
  • Place of Presentation: 学士会館(東京都千代田区)
  • Year and Date: 2009-10-10
• [Presentation] A systems approach reveals myogenesis genome network is regulated by the transcriptional repressor RP58. (2009)
  • Author(s): Yokoyama S, et al.
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー(東京都)
  • Year and Date: 2009-05-15
• [Presentation] A systems approach reveals myogenesis genome network is regulated by the transcriptional repressor RP58 (2009)
  • Author(s): Yokoyama S, et al.
  • Organizer: 第7回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー(東京都港区)
  • Year and Date: 2009-05-15
• [Presentation] 骨・軟骨発生におけるmicroRNA-140の機能解析 (2009)
  • Author(s): 佐藤天平, 他
  • Organizer: 第22回日本軟骨代謝学
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-03-06
• [Presentation] 骨・軟骨発生におけるmicroRNA-140の機能解析 (2009)
  • Author(s): 佐藤天平, 他
  • Organizer: 第22回日本軟骨代謝学会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2009-03-06
• [Presentation] Systems approach reveals myogenesis molecular network anchored by transcription repressor. (2009)
  • Author(s): 浅原弘嗣, et al.
  • Organizer: Keystone Symposia (Epigenetics, Development and Human Disease (A1)).
  • Place of Presentation: Breckenridge, CO(米国)
  • Year and Date: 2009-01-06
• [Presentation] Systems approach reveals myogenesis molecular network anchored by transcription repressor (2009)
  • Author(s): Asahara H, et al.
  • Organizer: Keystone Symposia (Epigenetics, Development and Human Disease (A1))
  • Place of Presentation: Breckenridge, CO, USA
  • Year and Date: 2009-01-06
• [Presentation] Systems approach reveals transcription network regulated by Sox9. (2008)
  • Author(s): 浅原弘嗣
  • Organizer: 2nd International SOX Meeting.
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2008-09-18
• [Presentation] Systems approach reveals transcription network regulated by Sox9 (2008)
  • Author(s): Asahara H.
  • Organizer: 2nd International SOX Meeting
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場(兵庫県淡路市)
  • Year and Date: 2008-09-18
• [Presentation] Expression of miR-140 in human articular cartilage and during chondrogenesis. (2008)
  • Author(s): Miyaki S, et al.
  • Organizer: The 72nd annual meeting of the American College of Rheumatology and the 43nd annual meeting of the Association of Rheumatology Health Professionals.
  • Place of Presentation: San Francisco, CA(米国)
• [Presentation] Expression of miR-140 in human articular cartilage and during chondrogenesis (2008)
  • Author(s): Miyaki S, et al.
  • Organizer: The 72nd annual meeting of the American College of Rheumatology and the 43nd annual meeting of the Association of Rheumatology Health Professionals
  • Place of Presentation: San Francisco, CA, USA