Differences of susceptibility and aging of inner damage

• Project/Area Number: 20390444
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Keio University
• Principal Investigator: OGAWA Kaoru Keio University, 医学部 (00169179)
• Co-Investigator(Kenkyū-buntansha): TSUDA Reo 国立長寿センター, 老化機構研究部, 室長 (30333355) ; KANZAKI Sho 慶應義塾大学, 医学部, 講師 (50286556)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 易受傷性 / 内耳障害 / 有毛細胞 / 活性酸素 / オートファジー / 神経 / 聴覚 / 老化 / 酸化障害 / 音響暴露 / 加齢 / 医学 / 難聴

Research Abstract

We concluded that differences of free radical scavenger and autophagy between individuals can determine inner ear homeostasis. OGG (free radical scavenger gene) KO mice exposed to acoustic trauma demonstrated more hair cells damages compared to wild type mice as control. Autophagy 7 conditional knockout mice presented stereocilia loss. It may suggest that autophagy is critical factor for maintaining hair cells. One study is still ongoing research regarding functional and morphological analysis of TBL-1 KO mice which we proposed as presbycusis mice model

Research Products

• [Journal Article] A new device for delivering drugs into the inner ear (2011)
  • Author(s): Kanzaki S, Saito H, Inoue Y,Ogawa K
  • Journal Title: otoendoscope with microcatheter Auris Nasus Larynx (in press)
  • Peer Reviewed
• [Journal Article] 内視鏡による鼓室内投与法、耳科学会パネルディスカッション「感音難聴は鼓室内注入で治るか」 (2011)
  • Author(s): 神崎晶
  • Journal Title: Otology Japan 21(2)
• [Journal Article] 内視鏡による鼓室内投与法、耳科学会パネルディスカッション「感音難聴は鼓室内注入で治るか」 (2011)
  • Author(s): 神崎晶
  • Journal Title: Otology Japan Volume: (印刷中)
• [Journal Article] A new device for delivering drugs into the inner eotoendoscope with microcatheter (2011)
  • Author(s): Kanzaki S, Saito H, Inoue Y, Ogawa K
  • Journal Title: Auris Nasus Larynx Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Bisphosphonate Therapy Ameliorates Hearing Loss in Mice Lacking Osteoprotegerin (2009)
  • Author(s): Kanzaki s, Takada Y, Oqawa K Matsuo K
  • Journal Title: J Bone Miner Res. 24 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Pathogenesis of hearing loss from the point of view of the long-term prognosis of steroid-dependent sensorineural hearing loss. (2009)
  • Author(s): Kanzaki J, Kanzaki S, Ogawa K.
  • Journal Title: Audiol Neurotol 14 Pages: 26-34
  • Peer Reviewed
• [Journal Article] Detection system for transplanted bone marrow stem cell in inner ear SPlO. (2009)
  • Author(s): Yamashita D, Watada Y, Kanz S, Toyoda M, Oqawa K, et al
  • Journal Title: Neurosci Res (supplement) Pages: 25-25
• [Journal Article] Bone marrow stem cells therapy for cochlear hair cell and auditfunction. (2009)
  • Author(s): Kanzaki S, Yamashita D, Toyoda M, Kobayashi M, Ogawa K, et al
  • Journal Title: Neurosci Res 19
• [Journal Article] Inferior meatal antrostomy impairs dynamic stability of the orbital walls (2009)
  • Author(s): Nagasao T, Miyamoto J, Tamaki Kanzaki S, Oqawa K.
  • Journal Title: Auris Nasus Larynx (In press)
  • Peer Reviewed
• [Journal Article] In vivo delivery of adeno-associated virus was successfully anoninvasively transferred into the supporting cells of the neonatal moucochlea. (2008)
  • Author(s): lizuka T, Kanzaki S, Mochizuki H, Inoshita A, Oqawa K, et al
  • Journal Title: Human Gene Ther 19 Pages: 384-390
  • Peer Reviewed
• [Journal Article] Mucoepidermoid carcinoma of the head and neck : clinical analysis of patients. (2008)
  • Author(s): Ozawa H, (他4名, Kanzaki Oqawa K
  • Journal Title: J Clin Oncol. 38 Pages: 414-418
  • Peer Reviewed
• [Journal Article] GFAP aggregates in the cochlear nerve increase the noise vulnerabi of sensory ceils in the organ of Corti in the murine model of Alexandisease. (2008)
  • Author(s): Masuda M, Tanaka K, Kanzaki s, Ogawa K, et al
  • Journal Title: Neurosci Res. 62 Pages: 15-24
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of T-817MA against noise-induced hearing loss (2008)
  • Author(s): Yamashita D, Shiotani A, Kanzaki S Oaawa K
  • Journal Title: Neurosci Res 61 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Bcl-2 genes regulate noise-induced hearing loss. (2008)
  • Author(s): Yamashita D, Minami SB, Kanz S Oqawa K, et al
  • Journal Title: J Neurosci Res 86 Pages: 920-928
  • Peer Reviewed
• [Journal Article] standardised method of selecting surgical approaches to benparapharyng space tumours, based on pre-operative images. (2008)
  • Author(s): Kanzaki s, Nameki H.
  • Journal Title: J Laryngol Otol 122 Pages: 628-634
  • Peer Reviewed
• [Presentation] 内視鏡による鼓室内投与法、耳科学会パネルディスカッション「感音難聴は鼓室内注入で治るか」 (2010)
  • Author(s): 神崎晶
  • Organizer: 日本耳科学会
  • Place of Presentation: 愛媛県松山市ひめぎんホール
  • Year and Date: 2010-10-06
• [Presentation] The molecular mechanism of autophagy in auditory cells (2010)
  • Author(s): Hayashi K, Goto F, Kanzaki S, Ogawa K
  • Organizer: Annual Meeting American Academy of Otolaryngology-Head and Neck Surgery & OTO Expo
  • Place of Presentation: Boston, MA USA(poster award)
  • Year and Date: 2010-09-28
• [Presentation] The molecular mechanism of autophagy in auditory cells (poster award) (2010)
  • Author(s): Hayashi K, Goto F, Kanzaki S, Ogawa K
  • Organizer: Annual Meeting American Academy of Otolaryngology-Head and Neck Surgery & OTO Expo in Bosto
  • Place of Presentation: MA, USA(poster award)
• [Presentation] Noninvasive in vivo delivery of transgene via adeno-associated virus isupporting cells of the neonatal mouse cochlea (oral presentation) (2009)
  • Author(s): lizuka T, Kanzaki S, Oqawa Kal.
  • Organizer: ARO 32^<nd> Midwinter meeting
  • Place of Presentation: Baltimore USA
• [Presentation] 突発性難聴患者における初診時血液検査と聴力予後に関する検討 (2008)
  • Author(s): 神崎晶, 阪上雅史, 細井祐司, 村上信五, 吉原俊雄, 小川郁
  • Organizer: 第18回日本耳科学会
  • Place of Presentation: 神戸、日本
  • Year and Date: 2008-10-17
• [Presentation] Gene and cell delivery into inner ear (oral presentation) (2008)
  • Author(s): Kanzaki S
  • Organizer: Keio Medical society meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-10-14
• [Presentation] Symposia “Regeneration of Sensory Cells in the lnner Ear-From Ber to Bedside-", (oral) (2008)
  • Author(s): Kanzaki s, Yamashita D, Oqawa K
  • Organizer: 31^<st> Annual meeting Japan Neuroscience society meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-07-11
• [Presentation] Bisphosphonate therapy ameliorates bone resorption and hearing lossmice lacking osteoprotegerin"(oral presentation) (2008)
  • Author(s): Kanzaki S, Oqawa K
  • Organizer: 8^<th> Cholesteatoma and Ear Surgery meeting
  • Place of Presentation: Antalya, Turkey
  • Year and Date: 2008-06-17
• [Presentation] (セッション老人性難聴と基礎研究)骨粗鬆症と耳小骨の変化に関する検 (2008)
  • Author(s): 神崎晶, 小川郁
  • Organizer: 東京アンチエイジングアカデミーセミナー
  • Place of Presentation: 東京、日本
  • Year and Date: 2008-06-05
• [Presentation] The role of inflammation and apoptosis in sudden sensorineural hearinloss (oral presentation) (2008)
  • Author(s): Oqawa K, Kanzaki s, Okamoto Fujioka M, Matsunaga T
  • Organizer: Collegium, Oto-Rhino- Laryngologicum
  • Place of Presentation: Berlin, Germany
• [Book] 家庭の医学 外耳炎、外耳道湿疹 (2009)
  • Author(s): 神崎晶
  • Publisher: 小学館
• [Remarks] ホームページ等