| Project/Area Number |
20390446
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAMAKI Yasuhiro The University of Tokyo, 医学部附属病院, 准教授 (20217178)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGI Yasuo 東京大学, 医学部附属病院, 特任講師 (90376442)
KATAOKA Kazunori 東京大学, 大学院・工学系研究科, 教授 (00130245)
NISHIYAMA Nobuhiro 東京大学, 大学院・医学系研究科, 准教授 (10372385)
FURUKAWA Takahisa 大阪バイオサイエンス研究所, 発生生物学部門, 研究部長 (50260609)
KONDO Mineo 名古屋大学, 医学部附属病院, 准教授 (80303642)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | ナノバイオ / 移植・再生医療 / マイクロアレイ / 発生・分化 / トランスレーショナル・リサーチ |
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| Research Abstract |
We report that a major lipofuscin component, A2E (N-retinyledin-N- retinylethanolamin), activates the retinoic acid receptor (RAR). We have also developed a ternary complex composed of a core containing DNA packaged with cationic peptides and enveloped in the anionic dendrimer phthalocyanine, which provides the photosensitizing action. This system, PCI-mediated gene delivery, can be potentially useful for the genen therapy of ophthalmic disease.
We performed a comparative gene expression analysis between the central and peripheral regions of the monkey retina using monkey (Rhesus Macaque) genome microarray chips. We then selected the clones which were expressed at significantly higher levels in the central region and confirmed their expression in the monkey retina by section in situ hybridization. This study sheds light on the mechanisms of fovea development and may lead to the development of regenerative medicine for cone photoreceptors.
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