Demonstration of PACAP on protection of delayed neuronal cell death and its molecular mechanism of neuroregeneration.
Project/Area Number |
20390461
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Emergency medicine
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Research Institution | Showa University |
Principal Investigator |
SHIODA Seiji Showa University, 医学部, 教授 (80102375)
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Co-Investigator(Kenkyū-buntansha) |
OHTAKI Hirokazu 昭和大学, 医学部, 助教 (20349062)
NAKANACHI Tomoya 昭和大学, 遺伝子組換え実験室, 助教 (30433840)
荒田 悟 昭和大学, 遺伝子組換え実験室, 准教授 (20159502)
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Co-Investigator(Renkei-kenkyūsha) |
ARATA Satoru 昭和大学, 遺伝子組換え実験室, 准教授 (20159502)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2008: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
|
Keywords | PACAP / PAC1-R / IL-6 / 神経細胞死 / 神経再生 / PAC-1R |
Research Abstract |
We have studied to clarify the molecular basis for PACAP on protection of delayed neuronal cell death after brain ischemia in mice and to make a translational study for humans. I n addition, we have also studied the molecular mechanism of PACAP on stimulating differentiation from neural stem cells into neurons and/or glial cells. Moreover, we developed a new drug delivery system (DDS) of PACAP by use of intranasal infusion to protect delayed neuronal cell death after brain ischemia. The present study will have a strong impact for protection of brain ischemia and it will open a new avenue to use of PACAP and its related peptides for clinical use for patients who will be suffered from neurodegeneration after brain ischemia with a new developed DDS.
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Report
(4 results)
Research Products
(124 results)
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[Journal Article] Neuroprotective effect of PACAP against NMDA-induced retinal damage in the mouse.2011
Author(s)
Endo K, Nakamachi T, Seki T, Kagami N, Wada Y, Nakamura K, Kishimoto K, Hori M, Tsuchikawa D, Shinntani N, Hashimoto H, Baba A, Koide R, Shioda S
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Journal Title
J Mol Neurosci 43(1)
Pages: 22-9
Related Report
Peer Reviewed
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[Journal Article] Regulation of Oxidative Stress by Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Mediated by PACAP Receptor.2010
Author(s)
Ohtaki H, Satoh A, Nakamachi T, Yofu S, Dohi K, Mori H, Ohara K, Miyamoto K, Hashimoto H, Shintani N, Baba A, Matsunaga M, Shioda S
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Journal Title
J Mol Neurosci 42(3)
Pages: 397-403
Related Report
Peer Reviewed
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[Journal Article] Cardioprotective Effect of Endogenous Pituitary Adenylate Cyclase-Activating Polypeptide on Doxorubicin-Induced Cardiomyopathy in Mice.2010
Author(s)
Mori H, Nakamachi T, Ohtaki H, Yofu S, Sato A, Endo K, Iso Y, Suzuki H, Takeyama Y, hintani N, Hashimoto H, Baba A, Shioda S
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Journal Title
Circ J 74(6)
Pages: 1183-1190
NAID
Related Report
Peer Reviewed
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[Journal Article] Endogenous pituitary adenylate cyclase activating polypeptide is involved in suppression of edema in the ischemic brain.2010
Author(s)
Nakamachi T, Ohtaki H, Yofu S, Dohi K, Watanabe J, Mori H, Sato A, Hashimoto H, Shintani N, Baba A, Shioda S
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Journal Title
Acta Neurochir Suppl 106
Pages: 43-46
Related Report
Peer Reviewed
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[Journal Article] Isolation of peptide transport system-6 from brain endothelial cells : therapeutic effects with antisense inhibition in Alzheimer and stroke models.2009
Author(s)
Dogrukol-Ak D, Kumar VB, Ryerse JS, Farr SA, Verma S, Nonaka N, Nakamachi T, Ohtaki H, Niehoff ML, Edwards JC, Shioda S, Morley JE, Banks WA
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Journal Title
J Cereb Blood Flow Metab 29(2)
Pages: 411-422
Related Report
Peer Reviewed
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[Journal Article] Distribution of the longevity gene product, SIRT1, in developing mouse organs.
Author(s)
Ogawa T, Wakai C, Saito T, Murayama A, Mimura Y, Youfu S, Nakamachi T, Kuwagata M, Satoh K, Shioda S
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Journal Title
Congenit Anom (Kyoto) (in press)
Related Report
Peer Reviewed
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[Journal Article] Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Stimulates Proliferation of Reactive Astrocytes In Vitro.
Author(s)
Nakamachi T, Nakamura K, Oshida K, Kagami N, Mori H, Watanabe J, Arata S, Yofu S, Endo K, Wada Y, Hori M, Tsuchikawa D, Kato M, Shioda S
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Journal Title
J Mol Neurosci (in press)
Related Report
Peer Reviewed
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[Presentation] Effects of pituitary adenylate cyclase activating polypeptide (PACAP) in corneal epithelial regeneration andsignal transduction in rats.2011
Author(s)
Shioda S, J Farkas, L Mester, K Kovacs, D Reglodi, A Matkovits, V Gaal, Z Biro, A Szabo, B Racz, K Szabadfi, A Lubics, T Atlasz, A Tamas, T Nakamachi, P Kiss
Organizer
第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会 合同大会
Place of Presentation
横浜
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[Presentation] The roles of Gp91phox (NOX2) expressed in classical activated microglia after traumatic brain injury.2010
Author(s)
Dohi K, Ohtaki H, Kazue S, Nakamachi T, Yofu S, Miyamoto K, Song D, Tsunawaki S, Shioda S, Aruga T
Organizer
10th International Congress of Neuroimmunology (ISNI 2010)
Place of Presentation
Sites, Spain
Related Report
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[Presentation] Effect of pituitary adenylate cyclase activating polypeptide (PACAP) in corneal epithelial regeneration and signal transduction in rats.2010
Author(s)
Farkas J, Mester L, Kovacs K, Reglodi D, Matkovits A, Gaal V, Biro Z, Szabo A, Racz B Szabadfi K, Lubics A, Tamas A, Atlasz T, Shioda S, Nakamachi T, Kiss P
Organizer
5th International Peptide Symposium
Place of Presentation
Kyoto
Related Report
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[Presentation] Endogenous PACAP as a cardioprotectant in Doxorubicin-induced cardiomyopathy model in mice.2009
Author(s)
Mori H, Nakamachi T, Ohtaki H, Hasegawa F, Yofu S, Suzuki H, Takeyama Y, Shintani N, Hashimoto H, Baba A, Shioda S
Organizer
Yakushima 2009 (Satellite Symposium of the 9th International Symposium on VIP, PACAP and Related Peptides) Phylogenetic Aspects of Neuropeptides-from Invertebrates to Humans
Place of Presentation
Yakushima, Kagoshima
Related Report
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[Presentation] Effect of PACAP on tear secretion in mouse.2009
Author(s)
Nakamachi T, Aizawa Y, Ohtaki H, Yofu S, Seki T, Endo K, Nakamura K, Kagami N, Arata S, Shintani N, Hashimoto H, Baba A, Shioda S
Organizer
The 9th International Symposium on VIP, PACAP and Related Peptides
Place of Presentation
Kagoshima
Related Report
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[Presentation] Endogenous PACAP attenuated doxorubicin-induced myocardial damage.2009
Author(s)
Mori H, Nakamachi T, Ohtaki H, Hasegawa F, Yofu S, Suzuki H, Takeyama Y, Shintani N, Hashimoto H, Baba A, Shioda S
Organizer
The 9th International Symposium on VIP, PACAP and Related Peptides
Place of Presentation
Kagoshima
Related Report
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[Presentation] Suppression of oxidative stress by PACAP.2009
Author(s)
Sato A, Ohtaki H, Dohi K, Nakamachi T, Yofu S, Ohara K, Hashimoto H, Murakami T, Ando M, Shintani N, Baba A, Shioda S
Organizer
The 9th International Symposium on VIP, PACAP and Related Peptides
Place of Presentation
Kagoshima
Related Report
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[Presentation] Observation of the longevity gene product, SIRT1 immunoreactivity in the developing mouse nervous system.2009
Author(s)
Ogawa T, Wakai C, Saito T, Murayama A, Mimura Y, Youfu S, Kuwagata M, Nakamachi T, Sato K, Shioda S
Organizer
The 39th Annual Meeting of the Society for Neuroscience
Place of Presentation
Chicago, IL
Related Report
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