Spatiotemporal analysis of osteoclastogenesis during bone remodeling

• Project/Area Number: 20390463
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NAKAHAMA Kenichi Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 准教授 (60281515)
• Co-Investigator(Kenkyū-buntansha): MORITA Ikuo 東京医科歯科大学, 教授 (60100129) ; YOKOYAMA Chieko 東京医科歯科大学, COE特任教授 (90200914) ; SATO Takahiro 東京医科歯科大学, 寄付講座職員 (20397003) ; ICHINOSE Shizuko 東京医科歯科大学, 助教 (60015146) ; 市野瀬 志津子 東京医科歯科大学, 先端研究支援センター, 助教 (60014156)
• Co-Investigator(Renkei-kenkyūsha): AKIYAMA Masako 東京医科歯科大学, 特任助教 (30436646)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 骨芽細胞 / 骨細胞 / 破骨細胞 / コネキシン43 / cAMP / ギャップジンクション / ギャップジャンクション / RAW細胞 / 破骨細胞前駆細胞 / 破骨細胞分化 / 接着分子 / CD11b

Research Abstract

We aimed to find the roles of cell communication in bone remodeling. We found an important role of cell communication between osteoclast precursors in osteoclastogenesis via adhesion molecule. Next, we showed the possibility that the gap junctional intercellular communication between osteoblasts and osteocytes controlled osteoclastogenesis via cyclic AMP signaling.

Research Products

• [Journal Article] Amyloid β Enhances Migration of Endothelial Progenitor Cells by Upregulating CX3CR1 in Response to Fractalkine, Which May Be Associated With Development of Choroidal Neovascularization. (2011)
  • Author(s): Wang J, Ohno-Matsui K, Nakahama KI, Okamoto A, Yoshida T, Shimada N, Mochizuki M, Morita I.
  • Journal Title: Arterioscler Thromb Vasc Biol. in press.
  • Peer Reviewed
• [Journal Article] Optimized method for culturing outgrowth endothelial progenitor cells. (2011)
  • Author(s): N.Oshima-Sudo, Q.Li, Y.Hoshino, K.Nakahama, T.Kubota, I.Morita
  • Journal Title: Inflammation and Regeneration 31(2) Pages: 219-227
  • NAID: 130004943789
  • Peer Reviewed
• [Journal Article] Cell-printing and transfer technology applications for bone defects in mice. (2011)
  • Author(s): Tsugawa J, Komaki M, Yoshida T, Nakahama KI, Amagasa T, Morita I.
  • Journal Title: J Tissue Eng Regen Med. In press.
  • Peer Reviewed
• [Journal Article] Cellular communications in bone homeostasis and repair. (2010)
  • Author(s): Nakahama K.
  • Journal Title: Cell Mol Life Sci. 67(23) Pages: 4001-4009
  • Peer Reviewed
• [Journal Article] The effects of polyunsaturated fatty acids and their metabolites on osteoclastogenesis in vitro. (2010)
  • Author(s): Yuan J, Akiyama M, Nakahama K, Sato T, Uematsu H, Morita I.
  • Journal Title: Prostaglandins Other Lipid Mediat. 92(1-4) Pages: 85-90
  • Peer Reviewed
• [Journal Article] Implantation of capillary structure engineered by optical lithography improves hind limb ischemia in mice. (2010)
  • Author(s): Akahori T, Kobayashi A, Komaki M, Hattori H, Nakahama K, Ichinose S, Abe M, Takeda S, Morita I.
  • Journal Title: Tissue Eng Part A. 16(3) Pages: 953-959
  • Peer Reviewed
• [Journal Article] Cellular communications in bone homeostasis and repair (2010)
  • Author(s): Ken-ichi Nakahama
  • Journal Title: Cell.Mol.Life Sci. Volume: 67 Pages: 4001-4009
  • Peer Reviewed
• [Journal Article] The effects of polyunsaturated fatty acids and their metabolites on osteoclastogenesis in vitro. (2010)
  • Author(s): Yuan J, Akiyama M, NakahamaK, Sato T, Uematsu H, Morita I.
  • Journal Title: Prostaglandins Other Lipid Mediat. Volume: 92 Pages: 85-90
  • Peer Reviewed
• [Journal Article] Potential enhancement of osteoclastogenesis by severe acute respiratory syndrome coronavirus 3a/X1 protein. (2009)
  • Author(s): Obitsu S, Ahmed N, Nishitsuji H, Hasegawa A, Nakahama K, Morita I, Nishigaki K, Hayashi T, Masuda T, Kannagi M.
  • Journal Title: Arch Virol. 154(9) Pages: 1457-1464
  • Peer Reviewed
• [Journal Article] Regulation of chemokine gene expression by hypoxia via cooperative activation of NF-kappaB and histone deacetylase. (2009)
  • Author(s): Safronova O, Pluemsampant S, Nakahama K, Morita I.
  • Journal Title: Int J Biochem Cell Biol. 41(11) Pages: 2270-2280
  • Peer Reviewed
• [Journal Article] Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes. (2009)
  • Author(s): Kaneda M, Nomura SM, Ichinose S, Kondo S, Nakahama K, Akiyoshi K, Morita I.
  • Journal Title: Biomaterials. 30(23-24) Pages: 3971-3977
  • Peer Reviewed
• [Journal Article] The steady-state expression of connexin43 is maintained by the PI3K/Akt in osteoblasts. (2009)
  • Author(s): Bhattacharjee R, Kaneda M, Nakahama K, Morita I.
  • Journal Title: Biochem Biophys Res Commun. 382(2) Pages: 440-444
  • Peer Reviewed
• [Journal Article] The reduction in pigment epithelium-derived factor is a sign of malignancy in ovarian cancer expressing low-level of vascular endothelial growth factor. (2009)
  • Author(s): Tsuchiya T, Nakahama K, Asakawa Y, Maemura T, Tanaka M, Takeda S, Morita M, Morita I.
  • Journal Title: Gynecol Endocrinol. 25(2) Pages: 104-109
  • Peer Reviewed
• [Journal Article] The steady-state expression of connexin43 is maintained by the PI3K/Akt in osteoblasts. (2009)
  • Author(s): Bhattacharjee R, Kaneda M, Nakahama K, Morita I.
  • Journal Title: Biochem Biophys Res Commun. 382 Pages: 440-444
  • Peer Reviewed
• [Journal Article] Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes. (2009)
  • Author(s): Kaneda M, Nomura SM, Ichinose S, Kondo S, Nakahama K, Akiyoshi K, Morita I.
  • Journal Title: Biomaterials 23-24 Pages: 3971-3977
  • Peer Reviewed
• [Journal Article] The steady-state expression of connexin43 is maintained by the PI3K/Akt in osteoblasts (2009)
  • Author(s): Rajib Bhattacharjee
  • Journal Title: Biochemical and Biophysical Research Communications 382 Pages: 440-444
  • Peer Reviewed
• [Journal Article] The role of Mac-1 (CD11b/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-kappaB ligand. (2008)
  • Author(s): Hayashi H, Nakahama K, Sato T, Tuchiya T, Asakawa Y, Maemura T, Tanaka M, Morita M, Morita I.
  • Journal Title: FEBS Lett. 582(21-22) Pages: 3243-3248
  • Peer Reviewed
• [Journal Article] Altered function of factor I caused by amyloid beta : implication for pathogenesis of age-related macular degeneration from Drusen. (2008)
  • Author(s): Wang J, Ohno-Matsui K, Yoshida T, Kojima A, Shimada N, Nakahama K, Safranova O, Iwata N, Saido TC, Mochizuki M, Morita I.
  • Journal Title: J Immunol. 181(1) Pages: 712-720
  • Peer Reviewed
• [Journal Article] Vitamin K2 suppresses malignancy of HuH7 hepatoma cells via inhibition of connexin 43. (2008)
  • Author(s): Kaneda M, Zhang D, Bhattacharjee R, Nakahama K, Arii S, Morita I.
  • Journal Title: Cancer Lett. 263(1) Pages: 53-60
  • Peer Reviewed
• [Journal Article] The role of Mac-1 (CDllb/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-jB ligand (2008)
  • Author(s): Hidetaka Hayashi
  • Journal Title: FEBS Letters 582 Pages: 3243-3248
  • Peer Reviewed
• [Presentation] 破骨細胞分化におけるICAM-2の役割 (2010)
  • Author(s): (李香蘭), 穐山雅子, 中浜健一, 輿石太郎,竹田省、森田育男
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] ギャップジャンクションを介した破骨細胞分化抑制作用の検討 (2010)
  • Author(s): (中浜健一), 小沢仁, 鈴木岳史, 森田育男
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 破骨細胞分化におけるICAM・2の役割 (2010)
  • Author(s): 李香蘭, 穐山雅子, 中浜健一, 輿石太郎, 竹田省、森田育男
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] ギャップジャンクションを介した破骨細胞分化抑制作用の検討 (2010)
  • Author(s): 中浜健一, 小沢仁, 鈴木岳史, 森田育男
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 異なるタイプの細胞間GJICを介したcAMP量の変動 (2010)
  • Author(s): (小沢仁)、鈴木岳之、中浜健一、森田育男
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-18
• [Presentation] 破骨細胞分化に及ぼす多価不飽和脂肪酸の影響 (2010)
  • Author(s): Jizhong Yhan, 穐山雅子、中浜健一、佐藤孝浩、植松宏、森田育男
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-18
• [Presentation] The Role of C-terminus of Cx43 in Gap Junction Plaque Internalization (2010)
  • Author(s): (Praween Wayakanon), Rajib Bhattacharjee, Makato Kaneda, Ken-ichi Nakahama, Ikuo Morita
  • Organizer: 第20回細胞間ジャンクション研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-23
• [Presentation] 破骨細胞分化に及ぼす多価不飽和脂肪酸の影響 (2009)
  • Author(s): (Jizhong Yuan), 穐山雅子、中浜健一、佐藤孝浩、植松宏、森田育男
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] 異なるタイプの細胞間GJICを介したcAMP量の変動 (2009)
  • Author(s): 小沢仁、鈴木岳之、中浜健一、森田育男
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] The steady-state expres sion level of connexin43 is maintained by the PI3K/Akt pathway. (2009)
  • Author(s): (Bhattacharjee R), Kaneda M, Nakahama K, Morita I
  • Organizer: 10^<th> Internationa l Symposium on Mechanism of Vasodilatation
  • Place of Presentation: Matsushima, Miyagi, Japan
• [Presentation] The steady-state expression level of connexin43 is maintained by the PI3K/Akt pathway. (2009)
  • Author(s): Bhattacharjee R, Kaneda M, 中浜健一, 森田育男
  • Organizer: 10^<th> International Symposium on Mechanism of Vasodilatation
  • Place of Presentation: Matsushima, Miyagi, Japan
• [Presentation] 破骨細胞形成におけるMac-1, (CDllb/CD18)の役割について (2008)
  • Author(s): 林 秀隆
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-30
• [Presentation] 印刷技術を用いて羊膜に転写した骨芽細胞による骨再生療法.ポスター演題 (2008)
  • Author(s): (津川順一)、小牧基浩、中浜健一、天笠光雄、森田育男
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 破骨細胞形成におけるMac-1, (CD11b/CD18)の役割について.ポスター演題 (2008)
  • Author(s): (林秀隆)、中浜健一、森田育男
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] The role of Mac-1 (CD11/CD18) in osteoclast differentiation induced by receptor activator of nuclear factor-κB ligand (RANKLE). (2008)
  • Author(s): (Nakahama K), Hayashi H, Sato T, Morita I
  • Organizer: 48^<th> The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] Phosphatidylinositol 3-kinase akt regulates connexin43 expression through mRNA stability osteoblasts. (2008)
  • Author(s): (Bhattacharjee R), Kaneda M, Nakahama K, Morita I
  • Organizer: 48^<th> The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Remarks] ホームページURL
• [Remarks]
  • URL: http://www.tmd.ac.jp/dent/cell/cell-J.htm