Elucidation of functional significance of caveola in periodontal mechanoreceptor

• Project/Area Number: 20390464
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
• Co-Investigator(Kenkyū-buntansha): IZUMI Kenji 新潟大学, 医歯学系, 准教授 (80242436) ; INOUE Kayoko 新潟大学, 医歯学系, 特任准教授 (90303130) ; KAWANO Yoshiro 新潟大学, 医歯学系, 助教 (60303129) ; SUZUKI Akiko 新潟大学, 医歯学系, 特任助教 (70509538)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: カベオラ / カベオリン / 機械受容 / 歯根膜ルフィニ神経終末 / カルシウムイオン / 歯根膜 / ルフィニ神経終末 / 末梢神経再生 / 軸索-シュワン細胞相互作用 / 終末シュワン細胞 / GDNF / 機械受容器

Research Abstract

The Ruffini endings, a primary mechanoreceptor in the periodontal ligament, well-develop caveolae in their cell membrane. The present study examined the functional significance of caveolae in the periodontal Ruffini endings. The caveolae were consisted of caveolin-1 with diverse functions. A unique immuno-localization pattern of caveolin-1 and Ca^<2+>-pump suggested caveolae is involved in the quick elimination of intracellular Ca^<2+> which influx into the axon terminals in mechanotransduction.

Research Products

• [Journal Article] Detection of acid-sensing ionchannel 3(ASIC3) in periodontal Ruffini endings of mouse incisors (2011)
  • Author(s): Rahman F, Harada F, Saito I, Suzuki A, Kawano Y, Izumi K, Nozawa-Inoue K, Maeda T
  • Journal Title: Neurosci Lett Volume: 488(2) Pages: 173-177
• [Journal Article] Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors (2011)
  • Author(s): F.Rahman, T.Maeda, et al
  • Journal Title: Neuroscience Letters Volume: 488 Pages: 173-177
  • Peer Reviewed
• [Journal Article] Initial phase of neuropathic pain within a few hours after nerve injury in mice (2011)
  • Author(s): S.Komagata, T.Maeda, et al
  • Journal Title: J.Neuroscience Volume: 31 Pages: 4896-4905
  • Peer Reviewed
• [Journal Article] Genetic reduction of GABA(A) receptor gamma2 subunit expression potentiates the immobilizing action of isoflurane. (2010)
  • Author(s): Seo, Maeda, et al.
  • Journal Title: Neuroscience Letters Volume: 472 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Differential cell-specific location of Cav-1 and Ca(2+)-ATPase in terminal Schwann cells and mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisor (2009)
  • Author(s): Iizuka N, Suzuki A, Nozawa-Inoue K, Kawano Y, Nandasena BG, Okiji T, Maeda T
  • Journal Title: J. Anat Volume: 214(2) Pages: 267-274
• [Journal Article] GDNF expression in terminal schwann cells associated with the periodontal ruffini endings of the rat incisors during nerve regeneration (2009)
  • Author(s): Ohishi M, Harada F, Rahman F, Saito I, Kawano Y, Nozawa-Inoue K, Maeda T
  • Journal Title: Anat Rec Volume: 292(8) Pages: 1185-1191
• [Journal Article] GDNF expression in terminal schwann cells associated with the periodontal Ruffini endings of the rat incisors during nerve regeneration. (2009)
  • Author(s): Ohishi, Maeda, et al.
  • Journal Title: Anatomical Record Volume: 292 Pages: 1185-1191
  • Peer Reviewed
• [Journal Article] Behavioral and histological observations of sensory impairment caused by tight ligation of the trigeminal nerve in mice. (2009)
  • Author(s): Seino, Maeda, et al.
  • Journal Title: J.Neuroscience Methods Volume: 181 Pages: 67-72
  • Peer Reviewed
• [Journal Article] Differential cell-specific location of Cav-1 and Ca^<2+>-ATPase in terminal Schwann cells and mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisor. (2009)
  • Author(s): N. Iizuka, et al
  • Journal Title: Journal of Anatomy 214 Pages: 267-274
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of ENaCβ in the terminal Schwann cells associated with the per iodonta 1 Ruffini endings of the rat incisor (2009)
  • Author(s): H. Hitomi, et al.
  • Journal Title: Biomedical Research (印刷中)
  • Peer Reviewed
• [Journal Article] GDNF expression in terminal Schwann cells associated with the periodontal Ruffini endings of the rat incisors during regeneration (2009)
  • Author(s): M. Ohishi, et al.
  • Journal Title: Anatomical Record (印刷中)
  • Peer Reviewed
• [Presentation] Dental innervation (2010)
  • Author(s): T.Maeda
  • Organizer: Peradeniya大学歯学部シンポジウム
  • Place of Presentation: Peradeniya大学歯学部、Kandy, Sri Lanka(招待講演)
  • Year and Date: 2010-12-06
• [Presentation] Acid-sensing ion channel 3 expression in the periodontal Ruffini endings (2010)
  • Author(s): Rahman F, Harada F, Kawano Y, Ohishi M, Nozawa-Inoue K, Saito I, Maeda T
  • Organizer: 88th General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
• [Presentation] Acid-sensing ion channel 3 expression in the periodontal Ruffini endings (2010)
  • Author(s): F.Rahman, T.Maeda, et al
  • Organizer: 88th General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: バルセロナコンベンションセンター、Barcelona, Spain
• [Presentation] Multiple neurotrophins involve regeneration of the periodontal Ruffini endings after transection of rat inferior alveolar nerve (2010)
  • Author(s): M.Ohishi, T.Maeda, et al
  • Organizer: 40th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: サンディエゴコンベンションセンター、San Diego, USA
• [Presentation] Involvement of multiple neurotrophins in development/regeneration of the periodontal Ruffini endings. (2009)
  • Author(s): Maeda
  • Organizer: International Dental School Symposium
  • Place of Presentation: National Yang-Ming University, Taipei
  • Year and Date: 2009-12-08
• [Presentation] Immunolocalization of caveolin-1 and Ca2+-ATPase in the periodontal Ruffini endings (2008)
  • Author(s): Suzuki A, Nozawa-Inoue K, Kawano Y, Ajima H, Maeda T
  • Organizer: 38th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington, DC
• [Presentation] Immunolocalization of caveolin-1 and Ca^<2+>-ATPase in the periodontal Ruffini endings. (2008)
  • Author(s): A. Suzuki, et al.
  • Organizer: 38^<th> Society for Neuroscience
  • Place of Presentation: ワシントンDC(米国)
• [Book] 歯根膜の感覚機能.歯界展望別冊歯と歯列を守るための歯根膜活用術 (2011)
  • Author(s): 前田健康
  • Publisher: 医歯薬出版
• [Book] 口腔解剖学 (2009)
  • Author(s): 前田健康
  • Total Pages: 212
  • Publisher: 医歯薬出版
• [Remarks]
  • URL: http://www.dent.niigata-u.ac.jp/anatomy2/anatomy2.html
• [Remarks]
  • URL: http://www.dent.niigata-u.ac.jp/anatomy2/anatomy2.html