| Project/Area Number |
20390468
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
|
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| Research Institution | Tsurumi University |
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Principal Investigator |
SAITO Ichiro Tsurumi University, 歯学部, 教授 (60147634)
|
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| Co-Investigator(Kenkyū-buntansha) |
MISHIMA Kenji 鶴見大学, 歯学部, 准教授 (50275343)
INOUE Hiroko 鶴見大学, 歯学部, 講師 (50367306)
YAMADA Hiroyuki 鶴見大学, 歯学部, 講師 (90267542)
小原 久実 鶴見大学, 歯学部, 助教 (70454163)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 口腔病理学 / 唾液分泌障害 / 酸化ストレス / クラステリン / 唾液分泌 |
|---|
| Research Abstract |
Clusterin was detected in the cytoplasm and the cell culture supernatant. The cytoplasmic clusterin inhibited reactive oxygen species (ROS) and cell death induced by H_2O_2 treatment. However, clusterin in the culture supernatant or recombinant clusterin could not inhibit ROS production. The clusterin-adenovirus was infected into salivary side population (SP) cells, which transferred into irradiated mouse salivary glands. The irradiated mice revealed defect of salivation, whereas the hyposalivation was restored in the mouse transferred clusterin-Ad infected SP cells. The SP cells from clusterin knock out mice or infected with clusterin shRNA-adenovirus failure to restore the salivation in the irradiated mice. These data suggested that clusterin is useful factor which recover the salivation from ROS induced hyposalivation.
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