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Regulatory mechanisms of Ca^<2+> oscillations and transcription factor NFAT in osteoclastogenesis.

Research Project

Project/Area Number 20390475
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

OKABE Koji  Fukuoka Dental College, 歯学部, 教授 (80224046)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Fujio  福岡歯科大学, 歯学部, 講師 (60153938)
KAJIYA Hiroshi  福岡歯科大学, 歯学部, 講師 (80177378)
INOUE Ryuji  福岡大学, 医学部, 教授 (30232573)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Keywords破骨細胞 / Ca^<2+>オシレーション / TRPチャネル / NFAT / 破骨細胞分化 / RANKL / TRPV2 / TRPM7 / NFATc1 / PLC系 / store-operated Ca^<2+> entry / TRP分子 / Ca^<2+>輸送体 / silencin / 非選択性陽イオンチャネル
Research Abstract

The receptor activator of NFκB ligand (RANKL) induces Ca^<2+> oscillations and activates the Nuclear Factor of Activated T cells 1 (NFATc1) during osteoclast differentiation (osteoclastogenesis). Ca^<2+> oscillations are an important trigger signal for osteoclastogenesis, however the molecular basis of Ca^<2+> permeable influx pathways serving Ca^<2+> oscillations has not yet been identified. Using a DNA microarray, we found that Transient Receptor Potential Vanilloid channels 2 (TRPV2) are expressed significantly in RANKL-treated RAW264.7 cells (preosteoclasts) compared to untreated cells. Therefore, we further investigated the expression and functional role of TRPV2 on Ca^<2+> oscillations and osteoclastogenesis. In conclusion, Ca^<2+> oscillations in preosteoclasts are triggered by RANKL-dependent TRPV2 and SOCE activation and intracellular Ca^<2+> release. Subsequent activation of NFATc1 promotes osteoclastogenesis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (16 results) Remarks (2 results)

  • [Journal Article] Oral epithelial cells are activated via TRP channels.2011

    • Author(s)
      Wang B, Danjo A, Kajiya H, Okabe K, Kido M.A
    • Journal Title

      J Dent Res Vol.90

      Pages: 163-167

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies to C1C7 inhibit extracellular acidification-induced C1-currents and bone resorption activity in mouse osteoclasts.2011

    • Author(s)
      Ohgi K, Okamoto F, Kajiya H, Sakagami R, Okabe K
    • Journal Title

      Naunyn-Schmiedeberg's Arch Pharmacol Vol.383

      Pages: 79-90

    • Related Report
      2010 Final Research Report
  • [Journal Article] Oral epithelial cells are activated via TRP channels.2011

    • Author(s)
      Wang B, Danjo A, Kajiya H, Okabe K, Kido M.A
    • Journal Title

      J Dent Res

      Volume: 90 Pages: 163-167

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies to ClC7 inhibit extracellular acidification-induced Cl^- currents and bone resorption activity in mouse osteoclasts2011

    • Author(s)
      Ohgi K, Okamoto F, Kajiya H, Sakagami R, Okabe K
    • Journal Title

      Naunyn-Schmiedeberg's Arch Pharmacol

      Volume: 383 Pages: 79-90

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RANKL-induced TRPV2 expression regulates osteoclastgenesis via calcium oscillations.2010

    • Author(s)
      Kajiya H, Okamoto F, Nemoto T, Kimachi K, Goto T K, Nakayama S, Okabe K
    • Journal Title

      Cell Calcium Vol.48

      Pages: 260-296

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] RANKL-induced TRPV2 expression regulates osteoclastgenesis via calcium oscillations.2010

    • Author(s)
      Kajiya H, Okamoto F, Nemoto T, Kimachi K, Goto T K, Nakayama S, Okabe K
    • Journal Title

      Cell Calcium

      Volume: 48 Pages: 260-296

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Zoledoronic Acid Inhibits TNF-・Induced RA NK Expression and Migration in Osteoclast Precursors.2010

    • Author(s)
      Kimachi K, Ikebe T, Nakayama S, Okamoto F, Kajiya H, Okabe K
    • Organizer
      ASBMR 2010 Annual Meetin
    • Place of Presentation
      Metro Toronto Convention Centre (Toronto, Canada)
    • Year and Date
      2010-10-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Zoledoronic Acid Inhibits TNF-α Induced RANK Expression and Migration in Osteoclast Precursors.2010

    • Author(s)
      Kimachi K, Ikebe T, Nakayama S, Okamoto F, Kajiya H, Okabe K
    • Organizer
      ASBMR 2010 Annual Meeting
    • Place of Presentation
      Metro Toronto Convention Centre (Toronto, Canada)
    • Year and Date
      2010-10-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規抗C1C7抗体の破骨細胞C1C7C1-チャネルのC1-活性と骨吸収活性に対する抑制作用2010

    • Author(s)
      大城希美子、岡本富士雄、鍛治屋浩、根本哲臣、堤貴司、岡部幸司
    • Organizer
      第52回歯科基礎医学会
    • Place of Presentation
      タワーホール船橋(東京都)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規抗ClC7抗体の破骨細胞ClC7Cl^-チャネルのCl^-活性と骨吸収活性に対する抑制作用2010

    • Author(s)
      大城希美子、岡本富士雄、鍛治屋浩、根本哲臣、堤貴司、岡部幸司
    • Organizer
      第52回歯科基礎医学会
    • Place of Presentation
      タワーホール船橋(東京都)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 窒素含有ビスフォスフォネート(NBP)製剤のゾレドロ酸による破骨前駆細胞の分化・遊走能抑制作用2010

    • Author(s)
      来海慶一郎、鍛治屋浩、中山修二、根本哲臣、大城希美子、岡本富士雄、岡部幸司
    • Organizer
      第52回歯科基礎医学会
    • Place of Presentation
      タワーホール船橋(東京都)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 口腔上皮細胞における機能的なTRPV4チャネルの発現2010

    • Author(s)
      王冰、合島怜央奈、鍛治屋浩、岡本富士雄、岡部幸司、城戸瑞穂
    • Organizer
      第52回歯科基礎医学会
    • Place of Presentation
      タワーホール船橋(東京都)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 新規抗C1C7抗体は破骨細胞C1C7型C1-チャネルのC1-輸送と骨吸収活性を抑制する2010

    • Author(s)
      大城希美子、岡本富士雄、鍛治屋浩、根本哲臣、来海慶一郎、岡部幸司
    • Organizer
      第28回日本骨代謝学会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2010-07-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規抗ClC7抗体は破骨細胞ClC7型Cl^-チャネルのCl^-輸送と骨吸収活性を抑制する2010

    • Author(s)
      大城希美子、岡本富士雄、鍛治屋浩、根本哲臣、来海慶一郎、岡部幸司
    • Organizer
      第28回日本骨代謝学会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2010-07-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 口腔上皮におけるカプサイシン感受性細胞の解析2009

    • Author(s)
      城戸瑞穂、王冰、鍛治屋浩、岡部幸司、張旌旗、山座孝義
    • Organizer
      第51回歯科基礎医学会
    • Place of Presentation
      新潟コンベンションセンター(新潟市)
    • Year and Date
      2009-09-11
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] RANKL-induced expression of TRPV2 is involved in osteoclastogegesis via calcium oscillations.2009

    • Author(s)
      Kajiya H, Okamoto F, Nemoto T, Ohgi K, Okabe K
    • Organizer
      XXXVI th International Union of Physiological Sciences (IUPS)
    • Place of Presentation
      Kyoto International Conference Center (Kyoto)
    • Year and Date
      2009-07-29
    • Related Report
      2010 Final Research Report
  • [Presentation] RANKL-INDUCED EXPRESSION OF TRPV2 IS INVOLVED IN OSTEOCLASTOGENESIS VIA CALCIUM OSCILLATIONS2009

    • Author(s)
      H Kajiya, F Okamoto, T Nemoto, K Ohgi, K Okabe
    • Organizer
      XXXVI th International Union of Physiological Sciences (IUPS)
    • Place of Presentation
      Kyoto International Conference Center (Kyoto)
    • Year and Date
      2009-07-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] TRPV4の活性型変異はカルシウムオシレーションを早発させ、破骨細胞分化を促進する2009

    • Author(s)
      増山律子、鍛治屋浩、北浦英樹、岡部幸司、小守壽文
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2009-07-25
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] TRPV2を介する破骨細胞の分化調節機構2008

    • Author(s)
      岡部幸司
    • Organizer
      トランスポーターワークショップin福岡
    • Place of Presentation
      福岡大学(福岡市)
    • Year and Date
      2008-11-02
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 破骨細胞の分化トリガーシグナルCa^<2+>oscillationの構築にはTrannsient Receptor Potential subfamily V2 (TRPV2)が必要である2008

    • Author(s)
      鍛治屋浩、岡本富士雄、大城希美子、根本哲臣、岡部幸司
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Year and Date
      2008-10-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 前駆破骨細胞に発現するTRPV2はCa^<2+>オシレーションの構築分子として破骨細胞への分化を誘導する2008

    • Author(s)
      岡本富士雄、鍛治屋浩、大城希美子、根本哲臣、岡部幸司
    • Organizer
      第59回西日本生理学会
    • Place of Presentation
      熊本市民会館(熊本市)
    • Year and Date
      2008-10-03
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 破骨細胞の分化トリガーシグナル Ca^<2+>oscillation の構築には Trannsient Receptor Potential subfamily V2(TRPV2)が必要である2008

    • Author(s)
      鍛治屋浩、岡本富士雄、大城希美子、根本哲臣、岡部幸司
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.fdcnet.ac.jp/col/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.fdcnet.ac.jp/col/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2021-09-15  

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