| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Research Abstract |
The conditioned media(CM) harvested from human pulmonary squamous cell carcinoma(QG56), pulmonary small cell carcinoma(QG90) and gastric adenocarcinoma(MKN28) cultivated under hypoxic conditions(3% O2), enhanced the angiogenic activity in vitro more than those obtained under normoxic cultivation(20% O2). The total length of the tube structures formed by bovine capillary endothelial cells(BCEs) in the CM cultured at 3% O2 was about 1. 5(QG56 and MKN28) or 1. 9(QG90) times longer than that at 20% O2. Tube formation was diminished by the preincubation of CM with anti-basic fibroblast growth factor(bFGF) IgG. After performing the fractionations of the CM and the crude extracts of cell lysates cultured using a heparin-Sepharose column, the mitogenic activity in the CM from all cancer cells at 3% O2 was about 2 times higher than that in the CM at 20% O2, while it decreased in the cell lysates at 3% O2 to about 40% of those at 20% O2. This mitogenic activity of BCEs in the CM from all cancer cells was almost totally suppressed by anti-basic fibroblast growth factor(bFGF) IgG, but not with anti-vascular endothelial growth factor(VEGF) IgG. These results suggest that the hypoxic condition is an important cause for tumor angiogenesis by bFGF or bFGF-like molecule(s) derived from tumor cells.
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