Pathogenic mechanisms in apical periodontitis : innate immunity, acquired immunity and dendritic cell maturation
| Project/Area Number |
20390483
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
OKIJI Takashi Niigata University, 医歯学系, 教授 (80204098)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIBA Kunihiko 新潟大学, 医歯学系, 准教授 (30220718)
YOSHIBA Nagako 新潟大学, 医歯学総合病院, 講師 (10323974)
OHSHIMA Hayato 新潟大学, 医歯学系, 教授 (70251824)
SHIGETANI Yoshimi 新潟大学, 医歯学系, 助教 (80397132)
KANEKO Tomoatsu 新潟大学, 医歯学総合病院, 助教 (70345297)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 樹状細胞 / マクロファージ / 根尖性歯周炎 / 獲得免疫 / 自然免疫 / 免疫機能分子 / Toll-like receptors / レーザーマイクロダイセクション / MHCクラスII分子 / 抗原提示細胞 |
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| Research Abstract |
This study aimed to advance the understanding of the involvement of dendritic cells and immunoregulatory molecules in the pathogenic mechanisms in apical periodontitis. Results demonstrated that unsealed pulp exposure caused the upregulation of MHC class II molecules, CD86, CD83, TLR2 and TLR4 mRNAs in the periodontal ligament of rat molars, as revealed by laser microdissection and real time PCR. Moreover, by employing a whole tooth culture system of the rat molar, it was demonstrated that resident macrophages and dendritic cells upregulated the expression of CD14, TLR4 and CX3CR1 following LPS stimulation.
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Report
(4 results)
Research Products
(18 results)
