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Pathogenic mechanisms in apical periodontitis : innate immunity, acquired immunity and dendritic cell maturation

Research Project

Project/Area Number 20390483
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNiigata University

Principal Investigator

OKIJI Takashi  Niigata University, 医歯学系, 教授 (80204098)

Co-Investigator(Kenkyū-buntansha) YOSHIBA Kunihiko  新潟大学, 医歯学系, 准教授 (30220718)
YOSHIBA Nagako  新潟大学, 医歯学総合病院, 講師 (10323974)
OHSHIMA Hayato  新潟大学, 医歯学系, 教授 (70251824)
SHIGETANI Yoshimi  新潟大学, 医歯学系, 助教 (80397132)
KANEKO Tomoatsu  新潟大学, 医歯学総合病院, 助教 (70345297)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords樹状細胞 / マクロファージ / 根尖性歯周炎 / 獲得免疫 / 自然免疫 / 免疫機能分子 / Toll-like receptors / レーザーマイクロダイセクション / MHCクラスII分子 / 抗原提示細胞
Research Abstract

This study aimed to advance the understanding of the involvement of dendritic cells and immunoregulatory molecules in the pathogenic mechanisms in apical periodontitis. Results demonstrated that unsealed pulp exposure caused the upregulation of MHC class II molecules, CD86, CD83, TLR2 and TLR4 mRNAs in the periodontal ligament of rat molars, as revealed by laser microdissection and real time PCR. Moreover, by employing a whole tooth culture system of the rat molar, it was demonstrated that resident macrophages and dendritic cells upregulated the expression of CD14, TLR4 and CX3CR1 following LPS stimulation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (18 results)

All 2011 2010 2009 2008 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (7 results) Book (2 results)

  • [Journal Article] Laser capture microdissection in dentistry2011

    • Author(s)
      Chokechanachaisakul U
    • Journal Title

      International Journal of Dentistry

      Volume: 2010

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical analysis of subcutaneous tissue reactions to meth acrylate resin-based root canal sealers2011

    • Author(s)
      Yamanaka Y
    • Journal Title

      International Endodontic Journal

      Volume: 44 印刷中

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased gene expression of toll-like receptors and antigen presenting cell-related molecules in the onset of experimentally induced furcation lesions of endodontic origin in rat molars2010

    • Author(s)
      Chokechanachaisakul U, Kaneko T, Okiji T, Kaneko R, Kaneko M, Kawamura J, Sunakawa M, Suda H
    • Journal Title

      J Endod 36(2)

      Pages: 251-255

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Laser Capture Microdissection in Dentistry2010

    • Author(s)
      Chokechanachaisakul U, Kaneko T, Okiji T, Kaneko R, Suda H, Nor JE
    • Journal Title

      International Journal of Dentistry. vol.2010, Article ID 592694

      Pages: 8-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased gene expression of Toll-like receptors and antigen-presenting cell-related molecules in the onset of experimentally induced furcation lesions of endodontic origin in rat molars.2010

    • Author(s)
      Chokechanachaisakul U
    • Journal Title

      Journal of Endodontics 36

      Pages: 251-255

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression analysis of acutely traumatized pulps2010

    • Author(s)
      Kaneko T
    • Journal Title

      Journal of Endodontics 36

      Pages: 78-82

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression analysis of immunostained endothelial cells isolated from formaldehyde-fixated paraffin embedded tumors using laser capture microdissection-a technical report.2009

    • Author(s)
      Kaneko T, Okiji T, Kaneko, R. Suda, H, Nor, JE
    • Journal Title

      Microsc Res Tec 72

      Pages: 908-912

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ラット臼歯に誘発した根分岐部病変の初期発症過程における免疫機能分子およびToll-likeレセプターmRNA発現の亢進2009

    • Author(s)
      金子友厚
    • Journal Title

      日本歯科保存学雑誌 52(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ラット正常歯根膜における抗原提示細胞の多様性-免疫機能分子およびToll-like receptor 4 mRNA発現の定量解析-2008

    • Author(s)
      金子友厚
    • Journal Title

      日本歯科保存学雑誌 51

      Pages: 502-507

    • NAID

      110007151260

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] A new method of culturing rat dental pulp tissue.2011

    • Author(s)
      Chokechanachaisaku U, Yamanaka Y, Kaneko T, Katsube K-I, Kobayashi H, OKIJI T, SUDA H
    • Organizer
      89th General Session & Exhibition of the IADR
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2011-03-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Anew method of culturing rat dental pulp tissue2011

    • Author(s)
      Chokechanachaisakul U
    • Organizer
      89th General Session of IADR
    • Place of Presentation
      San Diego
    • Year and Date
      2011-03-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dentin sialophosphoprotein expression in dental pulp tissue engineered with stem cells from exfoliated deciduous teeth2010

    • Author(s)
      Kaneko T
    • Organizer
      国際歯科研究学会日本部会
    • Place of Presentation
      小倉市
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Tissue alteration of rat dental pulp in whole tooth culture.2010

    • Author(s)
      Yoshiba K, Yoshiba N, Shigetani Y, Hosoya A, Okiji T
    • Organizer
      88th General Session & Exhibition of the IADR
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Temporal Changes in mRNA expression of mineralized tissue matrix proteins in GaAlAs laser-irradiated rat molars2010

    • Author(s)
      Shigetani Y, Ohkura N, Hosoya A, Yoshiba N, Yoshiba K, Ohshima H, Okiji T
    • Organizer
      IFEA 8th Endodontic World Congress.
    • Place of Presentation
      Athens, Greece
    • Related Report
      2010 Final Research Report
  • [Presentation] Analyses for dendritic cells in pulp infection-induced furcal inflammation.2009

    • Author(s)
      河村隼, 金子友厚, チョクチャナイサクンウライワン, 興地隆史, 砂川光宏、須田英明
    • Organizer
      日本顕微鏡学会第65回学術講演会
    • Place of Presentation
      仙台
    • Year and Date
      2009-05-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 露髄によって実験的に惹起されたラット臼歯根分岐部歯根膜の炎症部におけるToll様受容体mRNA発現の亢進2009

    • Author(s)
      Chokechanachaisakul U
    • Organizer
      日本歯科保存学会春季学会(第130回)
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2009 Annual Research Report
  • [Book] Laser capture microdissection from formaldehyde-fixated and demineralized paraffin embedded tissues. Microscopy : Science, Technology, Applications and Education volume 32010

    • Author(s)
      Kaneko T, Okiji T, Kaneko R, Suda H, Nor JE
    • Publisher
      Formatex Research Center
    • Related Report
      2010 Final Research Report
  • [Book] Pulp as a connective tissue(Hargreaves K, Goodis H eds.)(Seltzer and Bender's dental pulp, 2nd ed.)

    • Author(s)
      Okiji T
    • Publisher
      Quintessence, Chicago(in press)
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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