Evaluation of potential to promote the differentiation of osteoblastic cells by immobilized cationic antimicrobial peptide, JH8194 which promotes the proliferation of mesenchymal stem cells

• Project/Area Number: 20390499
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: NIKAWA Hiroki Hiroshima University, 大学院・医歯薬総合研究科, 教授 (10228140)
• Co-Investigator(Kenkyū-buntansha): MAKIHIRA Seicyo 九州大学, 大学院・歯学研究院, 准教授 (80304450)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 塩基性ペプチド / チタン / インプラント / 骨芽細胞 / オッセオインテグレーション / 多孔質

Research Abstract

The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration

Research Products

• [Journal Article] Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1. (2010)
  • Author(s): Makihira S, Shuto T, Nikawa H, Okamoto K, Mine Y, Takamoto Y, Ohara M, Tsuji K.
  • Journal Title: Int J Mol Sci. 11(4) Pages: 1458-1470
  • NAID: 120002577221
  • Peer Reviewed
• [Journal Article] Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1. (2010)
  • Author(s): Makihira S, Shuto T, Nikawa H, Okamoto K, Mine Y, Takamoto Y, Ohara M, Tsuji K.
  • Journal Title: Int J Mol Sci. Volume: 11(4) Pages: 1458-70
  • NAID: 120002577221
  • Peer Reviewed
• [Presentation] 歯科用インプラントとチタンの表面処理 (2009)
  • Author(s): 二川浩樹, 牧平清超, 西村正弘, 高萩隆行, 京極秀樹
  • Organizer: 広島大学ナノデバイス・バイオ融合科学研究シンポジウム集積医科学研究部門
  • Place of Presentation: 東広島市
  • Year and Date: 2009-05-27
• [Presentation] 歯科用インプラントとチタンの表面処理-歯科材料を中心とした生体材料開発の最前線- (2008)
  • Author(s): 二川浩樹
  • Organizer: 第34回材料制御研究会 中四国支部会(主催 日本金属学会・日本鉄鋼協会中国四国支部・広島大学歯学部口腔保健学科)
  • Place of Presentation: 広島市
  • Year and Date: 2008-12-26
• [Presentation] 塩基性抗菌ペプチドを応用した骨再生 (2008)
  • Author(s): 二川浩樹, 牧平清超
  • Organizer: 第2回口腔からQOLを目指す連携研究 広島大学(骨再生G)
  • Place of Presentation: 広島市
  • Year and Date: 2008-06-26
• [Presentation] 塩基性抗菌性ペプチドの応用と固定化抗菌剤を用いた洗剤開発の2つの事例から (2008)
  • Author(s): 二川浩樹
  • Organizer: 日本補綴歯科学会 公募型シンポジウム2
  • Place of Presentation: 名古屋
  • Year and Date: 2008-06-07
• [Presentation] 塩基性抗菌ペプチドの応用と新規インプラントの可能性 (2008)
  • Author(s): 二川浩樹
  • Organizer: 人工歯根研究会(中国地域ニュービジネス協議会)
  • Place of Presentation: 広島市
  • Year and Date: 2008-03-03