Functional analysis of microRNA in oral carcinoma.

• Project/Area Number: 20390507
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Chiba University
• Principal Investigator: SHIIBA Masashi Chiba University, 医学部附属病院, 講師 (20301096)
• Co-Investigator(Kenkyū-buntansha): YOKOE Hidetaka 防衛医科大学校, 医学部, 准教授 (70261930) ; BUKAWA Hiroki 筑波大学, 医学部, 教授 (80173558)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: microRNA / 口腔癌 / マイクロアレイ解析 / 癌関連遺伝子 / micro RNA / 癌関連遣伝子

Research Abstract

The purpose of this study was to identify crucial microRNAs (miRNAs) in oral squamous cell carcinoma (OSCC), and to reveal their biological function and clinical significance. To clarify whether miRNAs are involved in OSCC, global screening using miRNA microarray analysis was performed with OSCC-derived cell lines. The altered expression profiling was validated by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. MicroRNA microarray and real-time qRT-PCR analyses identified significantly up-regulated expression of miR-196a, and down-regulated expression of miR-125b, miR-222, let-7b and let-7c in OSCC-derived cell lines. Decreased expression of miR-125b was validated in 43 of 50 OSCC samples using real-time qRT-PCR and was revealed the association with cancer cell proliferation. This relationship was confirmed by sequential experiment using transfectants, in which expression of miR-125b was elevated.