| Project/Area Number |
20390522
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
KOTARO Tanimoto Hiroshima University, 病院, 講師 (20322240)
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| Co-Investigator(Kenkyū-buntansha) |
TANNE Kazuo 広島大学, 大学院・医歯薬学総合研究科, 教授 (30159032)
NOBUAKI Tanaka 広島大学, 大学院・医歯薬学総合研究科, 助教 (90397969)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Eiji 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40273693)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | エナメル蛋白 / バイオミネラリゼーション / エナメル質形成不全症 / アメロゲニン / ハイドロキシアパタイト |
|---|
| Research Abstract |
The purpose of this study was to investigate the effects of mutation of amelgenin, an enamel matrix protein, on the enamel crystal formation, and to elucidate of onset mechanism of amelogenesis imperfecta (AI).
Wild-type recombinant human full-length amelogenin and mutated recombinant human full-length ameogenin (P41T) were synthesized. There was a difference in the aggregation ability in solution at neutral pH (pH 7.4) between the wild-type and mutated full-length amelogenins. In addition, recombinant human MMP20 wa synthesized, and the digestion of recombinant human full-length amelogenin by the MMP20 was investigated. The digestion of mutated recombinant human full-length amelogenin was delayed as compared with that in wild-type amelogenin.
From these results, it was strongly suggested that a mutation of amelogenin may induce the abnormality of enamel structure during tooth formation.
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