Creation of the new periodontal tissue engineering by dual delivery

• Project/Area Number: 20390527
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tohoku University
• Principal Investigator: HIDETOSHI Shimauchi 東北大学, 大学院・歯学研究科, 教授 (70187425)
• Co-Investigator(Kenkyū-buntansha): EIJI Nemoto 東北大学, 大学院・歯学研究科, 講師 (40292221) ; KANAYA Sousuke 東北大学, 病院, 医員 (80375097) ; SUZUKI Osamu 東北大学, 大学院・歯学研究科, 教授 (60374948) ; SASANO Yasuyuki 東北大学, 大学院・歯学研究科, 教授 (30196191) ; KAMAKURA Shinji 東北大学, 大学院・医工学研究科, 教授 (80224640)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2008: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
• Keywords: 歯周組織再生 / スキャフォールド / サイトカイン / 細胞外微小環境 / 歯学 / 再生医学 / 遺伝子発現 / 歯周組織 / 骨伝導 / 細胞内シグナル / 遺伝子導入 / ティッシュエンジニアリング / bFGF(FGF-2) / カルシウム受容体 / デリバリーシステム

Research Abstract

The purpose of this study is to develop the new concept of periodontal tissue regeneration which has a high-efficiency and is propagable to general dentistry. It was revealed that an application of newly-developed scaffold induced the proliferation and control of extracellular inorganic ion concentration induced the expression of a variety of cell growth factors, resulting in the enhanced proliferation and differentiation of periodontal component cells, as well as other hard tissue forming cells. Our study strongly suggested a possibility for the development of periodontal tissue engineering by applying controlled release of inorganic ions and surface topography of scaffold combined with cytokines, without using cell therapy.

Research Products

• [Journal Article] Phosphate increases bone morphogenetic protein-2 expression through cAMP-dependent protein kinase and ERK1/2 pathways in human dental pulp cells (2011)
  • Author(s): Tada H, Nemoto E, Foster BL, Somerman MJ, Shimauchi H.
  • Journal Title: Bone Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Phosphate increases bone morphogenetic protein-2 expression through cAMP-dependent protein kinase and ERK1/2 pathways in human dental pulp cells (2011)
  • Author(s): Tada H, Nemoto E, Foster BL, Somerman MJ, Shimauchi H
  • Journal Title: Bone Volume: 48(印刷中)
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases fibroblast growth factor-2 gene and protein expression levels via a cAMP/PKA dependent pathway in cementoblasts (2010)
  • Author(s): Kanaya S, Nemoto E, Ebe Y, Somerman MJ, Shimauchi H.
  • Journal Title: Bone Volume: 47(3) Pages: 564-572
  • NAID: 130004571423
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells (2010)
  • Author(s): Tada H, Nemoto E, Kanaya S, Hamaji N, Sato H, Shimauchi H.
  • Journal Title: Biochem Biophys Res Commun Volume: 394(4) Pages: 1093-1097
  • Peer Reviewed
• [Journal Article] Comparative study on bone regeneration by synthetic octacalcium phospate with various granule sizes (2010)
  • Author(s): Murakami Y, Jonda Y, Anada T, Shimauchi H, Suzuki O.
  • Journal Title: Acta Biomateria Volume: 6 Pages: 1542-1548
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases fibroblast growth factor-2 gene and protein expression levels via a cAMP/PKA dependent pathway in cementoblasts (2010)
  • Author(s): Kanaya S, Nemoto E, Ebe Y, Somerman MJ, Shimauchi H
  • Journal Title: Bone Volume: 47 Pages: 564-572
  • NAID: 130004571423
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases expression of bone morphogenetic protem-2 gene via a calcium channel and ERK pathway in human dental pulp cells (2010)
  • Author(s): Tada H, Nemoto E, Kanaya S, Hamaji N, Sato H, Shimauchi H
  • Journal Title: Biochem Biophys Res Commun Volume: 394 Pages: 1093-1097
  • Peer Reviewed
• [Journal Article] Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells. (2010)
  • Author(s): Tada H, Nemoto E, Kanaya S, Hamaji N, Sato H, Shimauchi H
  • Journal Title: Biochemical and biophysical research communications 394 Pages: 1093-1097
  • Peer Reviewed
• [Journal Article] Comparative study on bone regeneration by synthetic octacalcium phosphate with various granule sizes. (2010)
  • Author(s): Murakami Y, Honda Y, Anada T, Shimauchi H, Suzuki O
  • Journal Title: Acta Biomateriana 6 Pages: 1542-1548
  • Peer Reviewed
• [Journal Article] Wnt signaling inhibits cementoblast differentiation and promotes proliferation (2009)
  • Author(s): Nemoto E, Koshikawa Y, Kanaya S, Tsuchiya M, Tamura M, Somerman MJ, Shimauchi H.
  • Journal Title: Bone Volume: 44(5) Pages: 805-812
• [Journal Article] Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electronmicroscopy (2009)
  • Author(s): Kaneko R, Akita H, Shimauchi H, Sasano Y.
  • Journal Title: J Electron Microsc (Tokyo) Volume: 58(6) Pages: 363-373
  • Peer Reviewed
• [Journal Article] Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects (2009)
  • Author(s): T Ebina H, Hatakeyama J, Onodera M, Honma T, Kamakura S, Shimauchi H, Sasano Y.
  • Journal Title: Oral Dis Volume: 15(4) Pages: 273-280
  • Peer Reviewed
• [Journal Article] Wnt signaling inhibits cementoblast differentiation and promotes proliferation. (2009)
  • Author(s): Nemoto E, Koshikawa Y, Kanaya S, Tsuchiya M, Tamura M, Somerman MJ, Shimauchi H
  • Journal Title: Bone 44 Pages: 805-812
  • Peer Reviewed
• [Journal Article] Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects (2009)
  • Author(s): Ebina H, Hatakeyama J, Onodera M, Honma T, Kamakura S, Shimauchi H, Sasano Y.
  • Journal Title: Oral Diseases 15(印刷中)
  • Peer Reviewed
• [Journal Article] Wnt signaling inhibits cementoblast differentiation and promotes proliferation (2009)
  • Author(s): Nemoto E, Koshikawa Y, Kanaya S, Tsuchiya M, Tamura M, Sommerman MS, Shimauchi H.
  • Journal Title: Bone 44(印刷中)
  • Peer Reviewed
• [Presentation] Preparation and Biomedical application of self-organized honeycomb-patterned polymer films (2011)
  • Author(s): Iwama N, Ishihata H, Kawano T, Shimauchi H, Shimomura M.
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台, 宮城
  • Year and Date: 2011-03-08
• [Presentation] Extracellular phosphate increases bone morphogenic protein-2 expression in human dental pulp cells and human periodontal ligament cells. (2011)
  • Author(s): Nemoto E, Tada T, Shimauchi H.
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台, 宮城
  • Year and Date: 2011-03-08
• [Presentation] Extracellular phosphate increases bone morphogenic protein-2 expression in human dental pulp cells and human periodontal ligament cells (2011)
  • Author(s): Nemoto E, Tada T, Shimauchi H
  • Organizer: The 4^<th> International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-08
• [Presentation] Preparation and Biomedical application of self-organized honeycomb-patterned polymer films (2011)
  • Author(s): Iwama N, Ishihata H, Kawano T, Shimauchi H, Shimomura M
  • Organizer: The 4^<th> International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-07
• [Presentation] Behavior of Human Periodontal Ligament Cells Cultured on Micro-arrayed Honeycomb-Patterned Film (2010)
  • Author(s): Iwama N, Ishihata H, Shimauchi H, Shimomura M.
  • Organizer: Asian Conference on Nanoscience and Nanotechnology Joint Conference with RIKEN-Asian Research Network Symposium
  • Place of Presentation: 東京, 日本
  • Year and Date: 2010-11-01
• [Presentation] Behavior of Human Periodontal Ligament Cells Cultured on Micro-arrayed Honeycomb-Patterned Film (2010)
  • Author(s): Iwama N, Ishihata H, Shimauchi H, Shimomura M
  • Organizer: Asian Conference on Nanoscience and Nanotechnology Joint Conference with RIKEN-Asian Research Network Symposium
  • Place of Presentation: 東京都
  • Year and Date: 2010-11-01
• [Presentation] マイクロプレシジョンハニカムスキャフォールドにより生成される歯根膜培養細胞グラフトを用いた歯周組織再生 (2010)
  • Author(s): 石幡浩志, 岩間張良, 下西充, 下村政嗣, 島内英俊
  • Organizer: 日本機械学会2010年度年次大会
  • Place of Presentation: 名古屋市, 愛知
  • Year and Date: 2010-09-06
• [Presentation] マイクロプレシジョンハニカムスキャフォールドにより生成される歯根膜培養細胞グラフトを用いた歯周組織再生 (2010)
  • Author(s): 石幡浩志, 岩間張良, 下西充, 下村政嗣, 島内英俊
  • Organizer: 日本機械学会2010年度年次大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-09-06
• [Presentation] Microtopographic Effect of Honeycomb Film on the Periodontal Ligament Cells (2010)
  • Author(s): Iwama N, Ishihata H, Ara M, Shimonishi M. Shimomura M, Shimauchi H.
  • Organizer: IADR General Session
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16
• [Presentation] セメント芽細胞におけるプロテインキナーゼC依存性Fibroblast Growth Factor-2発現増強作用 (2010)
  • Author(s): 金谷聡介, 根本英二, 後藤和宏, 島内英俊
  • Organizer: 日本歯科保存学会平成22年度春季学術大会(第132回)
  • Place of Presentation: 熊本市, 熊本
  • Year and Date: 2010-06-04
• [Presentation] セメント芽細胞におけるプロテインキナーゼC依存性Fibroblast growth factor 2発現増強作用 (2010)
  • Author(s): 金谷聡介, 根本英二, 後藤和宏, 島内英俊
  • Organizer: 日本歯科保存学会平成22年度春季学術大会(第132回)
  • Place of Presentation: 熊本市
  • Year and Date: 2010-06-04
• [Presentation] 細胞外リン酸はPitおよびERK1/2を介してヒト歯髄細胞からBMP-2を誘導する (2010)
  • Author(s): 多田浩之, 根本英二, 島内英俊
  • Organizer: 日本歯科保存学会平成22年度春季学術大会(第132回)
  • Place of Presentation: 熊本市
  • Year and Date: 2010-06-04
• [Presentation] セメント芽細胞において細胞外カルシウム刺激はcAMP/PKA依存性にFibroblast growth factor 2の発現を誘導する (2010)
  • Author(s): 金谷聡介, 根本英二, 江部由佳梨, 島内英俊
  • Organizer: 日本歯周病学会第53回春季学術大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-05-14
• [Presentation] Non-Canonical WNTシグナルが骨芽細胞の分化に与える影響について (2010)
  • Author(s): 江部由佳梨, 根本英二, 金谷聡介, 多田浩之, 島内英俊
  • Organizer: 日本歯周病学会第53回春季学術大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-05-14
• [Presentation] Proliferations of periodontal membrane cells on the unified topographic pattern of the honeycomb pored scaffold (2009)
  • Author(s): Shimauchi H, Tanaka M, Iwama N, Ishihata H, Ara M, Murakami Y, Nemoto E, Shimomura M.
  • Organizer: The 92nd AAP Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-09-15
• [Presentation] Proliferations of periodontal membrane cells on the unified topographic pattern of the honeycomb pored scaffold (2009)
  • Author(s): Shimauchi H, Tanaka M, Iwama N, Ishihata H, Ara M, Murakami Y, Nemoto E, Shimomura M
  • Organizer: The 92^<nd> AAP Annual Meeting
  • Place of Presentation: Boston, 米国
  • Year and Date: 2009-09-15
• [Presentation] Non-Canonical WNTシグナルが骨芽細胞の分化に与える影響について (2009)
  • Author(s): 江部由佳梨, 根本英二, 金谷聡介, 多田浩之, 島内英俊
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡市, 岩手
  • Year and Date: 2009-05-14
• [Presentation] セメント芽細胞において細胞外カルシウム刺激はcAMP/PKA依存性にFibroblast growth factor 2の発現を誘導する (2009)
  • Author(s): 金谷聡介, 根本英二, 江部由佳梨, 島内英俊
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡市, 岩手
  • Year and Date: 2009-05-14
• [Presentation] 細胞外マトリクス様ハニカムフィルム上におけるヒト歯根膜由来線維芽細胞の接着挙動に関する研究 (2008)
  • Author(s): 岩間張良, 田中賢, 石幡浩志, 荒雅浩下西充, 長峰勝, 村上宜央, 金谷聡介, 根本英二, 下村政嗣, 島内英俊
  • Organizer: 第51回秋季日本歯周病学会学術大会
  • Place of Presentation: 四日市, 三重
  • Year and Date: 2008-10-19
• [Presentation] 細胞外マトリクス様ハニカムフィルム上におけるヒト歯根膜由来線維芽細胞の接着挙動に関する研究 (2008)
  • Author(s): 岩間張良, 田中賢, 石幡浩志, 荒雅浩, 下西充, 長峰勝, 村上宜央, 金谷聡介, 根本英二, 下村政嗣, 島内英俊
  • Organizer: 第51回秋季日本歯周病学会学術大会
  • Place of Presentation: 三重県四日市市
  • Year and Date: 2008-10-19
• [Presentation] Elevated extracellular Ca2+ induces fibroblast growth factor-2 mRNA in cementoblasts (2008)
  • Author(s): Kanaya S, Nemoto E, Sommerman M, Shimauchi H.
  • Organizer: IADR / CADR 86^<th> General Session
  • Place of Presentation: Toronto, ON, CANADA
  • Year and Date: 2008-07-04