| Project/Area Number |
20390528
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
AMANO Atsuo Osaka University, 大学院・歯学研究科, 教授 (50193024)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Shinji 大阪大学, 大学院・歯学研究科, 助教 (40362678)
KATO Takahiro 大阪大学, 大学院・歯学研究科, 助教 (20423139)
FURUTA Nobumichi 大阪大学, 大学院・歯学研究科, 助教 (50452446)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIMORI Tamotsu 大阪大学, 大学院・医学研究科, 教授 (60191649)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | 歯学 / 歯周病菌 / 歯周病 / 感染症 / シグナル伝達 / 細菌・組織 / 動脈硬化 / 細胞・組織 / 細菌 |
|---|
| Research Abstract |
Porphyromonas gingivalis, the most well-known periodontal pathogen, can enter gingival epithelial cells and pass through the epithelial barrier into deeper tissues. However, it is poorly understood how this pathogen exits from infected cells for further transcellular spreading. The present study was performed to elucidate the cellular machinery exploited by P. gingivalis to exit from immortalized human gingival epithelial cells. P. gingivalis was internalized with early endosomes, and about half of the intracellular bacteria were found to be sorted to lytic compartments, including autolysosomes and late endosomes/lysosomes, while a considerable number of the remaining organisms were sorted to recycling endosomes. Small GTpases (Rab11 and RalA) and exocyst complex subunits (Sec5, Sec6, and Exo84) were also shown to be involved in the exit of P. gingivalis. The recycling pathway is exploited by intracellular P. gingivalis to exit from infected cells to neighboring cells as a mechanism of cell-to-cell spreading.
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