Periodontal system biology: analysis of molecular bases of periodontal disease associated genes.

• Project/Area Number: 20390529
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Osaka University
• Principal Investigator: YAMADA Satoru Osaka University, 歯学部・附属病院, 講師 (40359849)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Shinya 大阪大学, 大学院・歯学研究科, 教授 (70239490) ; KITAMURA Masahiro 大阪大学, 大学院・歯学研究科, 准教授 (10243247) ; YANAGITA Manabu 大阪大学, 大学院・歯学研究科, 助教 (80379081)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 歯周病学 / 歯根膜 / 歯根膜特異的Periostinアイソフォーム / グルタミン酸 / 歯周病関連遺伝子 / トランスジェニックマウス / PLAP-1 / 遺伝子型

Research Abstract

this study, we constructed the periodontal system biology network database (PsBND) and analyzed the PsBND database comparing with other gene expression databases in order to identify periodontal ligament-specific genes. We found novel Periostin isoforms, glutamate-signaling associated molecules induced by mechanical stress and PLAP-1 periodontal ligament-specific. Functional analysis revealed that these molecules harmonized to keep homeostasis of periodontal ligament tissues.

Research Products

• [Journal Article] Role of Mechanical stress-induced glutamate signaling-associated molecules in cytodifferentiation of periodontal ligament cells. (2010)
  • Author(s): Fujihara C, Yamada S, Ozaki N, Takeshita N, Kawaki H, Takano-Yamamoto T, Murakami S
  • Journal Title: J Biol Chem 285 Pages: 28286-28297
  • Peer Reviewed
• [Journal Article] Role of Mechanical stress-induced glutamate signaling-associated molecules in cytodifferentiation of periodontal ligament cells. (2010)
  • Author(s): Fujihara C, Yamada S, et al.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 28286-28297
  • Peer Reviewed
• [Presentation] Cathepsin K expression in human periodontal ligament cells (2011)
  • Author(s): Ozaki N, Yamada S, Fujihara C, Tauchi T, Kajikawa T, Awata T, Ozawa Y Murakami S
  • Organizer: 89^<th> IADR
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-18
• [Presentation] Mechanical stress induced glutamate signaling associated molecules regulate the cytodifferentiation of periodontal ligament cells (2010)
  • Author(s): 山田聡
  • Organizer: 韓国歯周病学会50周年記念大会
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2010-11-28
• [Presentation] ヒト歯根膜組織完全長cDNAライブラリーを用いたCathepsin Kの同定および機能解析 (2010)
  • Author(s): 尾崎亘弘、山田聡、藤原千春、田内拓史、梶川哲宏、粟田敏仁、小澤康宏、村上伸也
  • Organizer: 第133回日本歯科保存学会秋季学術大会
  • Year and Date: 2010-10-28
• [Presentation] ヒト歯根膜組織完全長cDNAライブラリーを用いたCathepsin Kの同定および機能解析 (2010)
  • Author(s): 尾崎亘弘
  • Organizer: 第133回日本歯科保存学会秋季学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2010-10-28
• [Presentation] Gene polymorphism in PLAP-1/asporin regulates mineralization of periodontal ligament cells (2010)
  • Author(s): 梶川哲宏, 山田聡, 小澤康宏, 藤原千春, 田内拓史, 尾崎亘弘, 村上伸也
  • Organizer: 88^<th> IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16
• [Presentation] Gene polymorphism in PLAP-1/asporin regulates mineralization of periodontal ligament cells (2010)
  • Author(s): Tetsuhiro Kajikawa
  • Organizer: 88^<th> IADR
  • Place of Presentation: バルセロナ、スペイン
  • Year and Date: 2010-07-16
• [Presentation] マウス歯周組織発生過程におけるPLAP-1タンパクの発現解析 (2010)
  • Author(s): 梶川哲宏、山田聡、小澤康宏、藤原千春、田内拓史、尾崎亘弘、村上伸也
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本市
  • Year and Date: 2010-06-04
• [Presentation] 新規歯根膜特異的PeriostinアイソフォームType IIの機能解析 (2010)
  • Author(s): 田内拓史、山田聡、前田憲一郎、藤原千春、梶川哲宏、尾崎亘弘、村上伸也
  • Organizer: 第53回春季日本歯周病学会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-05-14
• [Presentation] Cathepsin K expression in human periodontal ligament cells (2010)
  • Author(s): Nobuhiro Ozaki
  • Organizer: 89^<th> IADR
  • Place of Presentation: サンディエゴ、アメリカ
  • Year and Date: 2010-03-18
• [Presentation] グルタミン酸シグナルによる歯根膜細胞の分化制御 (2009)
  • Author(s): 藤原千春、山田聡、田内拓史、梶川哲宏、尾崎亘弘、小澤康宏、村上伸也
  • Organizer: 第131回日本保存学会秋季大会
  • Place of Presentation: 仙台市
  • Year and Date: 2009-10-30
• [Presentation] グルタミン酸シグナルによる歯根膜細胞の分化制御 (2009)
  • Author(s): 藤原千春
  • Organizer: 第131回日本歯科保存学会秋季大会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-30
• [Presentation] 歯周組織恒常性維持の分子基盤研究-歯根膜特異的分子PLAP-1の解析- (2009)
  • Author(s): 山田聡
  • Organizer: 第59回春季日本歯周病学会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-05-16
• [Presentation] 歯周組織恒常性維持の分子基盤研究-歯根膜特異的分子PLAP-1の解析- (2009)
  • Author(s): 山田聡
  • Organizer: 第59回春季日本歯周病学会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-16
• [Presentation] マウス歯周組織におけるPLAP-1タンパクの発現 (2009)
  • Author(s): 梶川哲宏、山田聡、小澤康宏、藤原千春、田内拓史、平野裕之、村上伸也
  • Organizer: 第59回春季日本歯周病学会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-05-15
• [Presentation] マウス歯周組織におけるPLAP-1タンパクの発現 (2009)
  • Author(s): 梶川哲弘
  • Organizer: 第59回春季日本歯周病学会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-15
• [Presentation] Periodontal ligament-specific periostin positively regulates mineralization of periodontal ligament cells (2009)
  • Author(s): Yamada S, Tauchi T, Fujihara C, Kajikawa T, Ozawa Y, Kitamura M, Murakami S
  • Organizer: 87^<th> IADR
  • Place of Presentation: Miami, USA
  • Year and Date: 2009-04-03
• [Presentation] Periodontal ligament-specific periostin positively regulates mineralization of periodontal ligament cells (2009)
  • Author(s): Satoru Yamada
  • Organizer: International Association of Dental Research (IADR) 87th General session
  • Place of Presentation: マイアミ、米国
  • Year and Date: 2009-04-03
• [Presentation] Mechanical stress-induced glutamate signaling in periodontal ligament cells (2008)
  • Author(s): Fujihara C, Yamada S, Yoneda S, Tauchi T, Kajikawa T, Ozawa Y, Murakami S
  • Organizer: 86^<th> IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-05