Role of multifunctional protein, GAPDH, associated with bacteria and human cells in infection by periodontopathic bacteria

• Project/Area Number: 20390534
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: NAGATA Hideki Osaka University, 大学院・歯学研究科, 准教授 (50260641)
• Co-Investigator(Kenkyū-buntansha): KUBONIWA Masae 大阪大学, 大学院・歯学研究科, 助教 (00303983) ; MAEDA Kazuhiko 大阪大学, 大学院・歯学研究科, 助教 (00346165)
• Co-Investigator(Renkei-kenkyūsha): KATAOKA Kosuke 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (50283792)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: GAPDH / 歯周病細菌 / ヒト細胞 / 感染 / 付着

Research Abstract

This study showed that a binding domain for P. gingivalis fimbriae may exist within amino acid residues 166 to 183 of S. oralis ATCC 9811 GAPDH. The peptide corresponding to amino acid residues 166 to 183 of S. oralis ATCC 9811 GAPDH (pep166-183) inhibited the interbacterial biofilm formation by several oral streptococci and P. gingivalis strains with different types of FimA. pep166-183 strongly inhibited biofilm formation between P. gingivalis and several oral bacteria which showed relatively strong cell surface-associated GAPDH activity. Moreover, we found that the binding domain for P. gingivalis fimbriae in human cell GAPDH may exist within the same domain as in S. oralis GAPDH.

Research Products

• [Journal Article] Genotyping to distinguish microbial pathogenicity in periodontitis (2010)
  • Author(s): M Kuboniwa, H Inaba, A Amano
  • Journal Title: Periodontology 2000 54 Pages: 136-159
  • Peer Reviewed
• [Journal Article] Subgingival biofilm formation (2010)
  • Author(s): M Kuboniwa, RJ Lamont
  • Journal Title: Periodontology 2000 52 Pages: 38-52
  • Peer Reviewed
• [Journal Article] Genotyping to distinguish microbial pathogenicity in periodontitis (2010)
  • Author(s): Masae Kuboniwa
  • Journal Title: Periodontology 2000 Volume: 54 Pages: 136-159
  • Peer Reviewed
• [Journal Article] Subgingival biofilm formation (2010)
  • Author(s): Masae Kuboniwa
  • Journal Title: Periodontology 2000 Volume: 52 Pages: 38-52
  • Peer Reviewed
• [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations (2009)
  • Author(s): M Kuboniwa, A Amano, H Inaba, E Hashino, S Shizukuishi
  • Journal Title: Oral Microbiology and Immunology 24 Pages: 260-263
  • Peer Reviewed
• [Journal Article] Identification of binding domain of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase for Porphyromonas gingivalis major fimbriae (2009)
  • Author(s): H Nagata, M Iwasaki, K Maeda, M Kuboniwa, E Hashino, M Toe, M Minamino, H Kuwahara, S Shizukuishi
  • Journal Title: Infection and Immunity 77 Pages: 5130-5138
  • Peer Reviewed
• [Journal Article] Distinct role of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis (2009)
  • Author(s): M Kuboniwa, A Amano, E Hashino, Y Yamamoto, H Inaba, N Hamada, K Nakayama, GD Tribble, RJ Lamont, S Shizukuishi
  • Journal Title: BMC Microbiology 9 Pages: 105-105
  • Peer Reviewed
• [Journal Article] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): M Kuboniwa, EL Hendrickson, Q Xia, T Wang, H Xie, M Hackett, RJ Lamont
  • Journal Title: BMC Microbiology 9 Pages: 98-98
  • Peer Reviewed
• [Journal Article] Distinct role of long/short fimbriae and gingipains in homotypic biofilmdevelopment by Porphyromonas gingivalis (2009)
  • Author(s): Masae Kuboniwa
  • Journal Title: BMC Microbiology 9 Pages: 105-105
  • Peer Reviewed
• [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations (2009)
  • Author(s): Masae Kuboniwa
  • Journal Title: Oral Microbiology and Immunology 24 Pages: 260-263
  • Peer Reviewed
• [Journal Article] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): Masae Kuboniwa
  • Journal Title: BMC Microbiology 9 Pages: 98-98
  • Peer Reviewed
• [Journal Article] Identification of binding domain of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase for Porphyromonas gingivalis major fimbriae (2009)
  • Author(s): Hideki Nagata
  • Journal Title: Infection and Immunity 77 Pages: 5230-5138
  • Peer Reviewed
• [Journal Article] A Porphyromonas gingivalis tyrosine phosphate is a multifunctional regulator of virulence attributes (2008)
  • Author(s): K Maeda, GD Tribble, CM Tucker, C Anaya, S Shizukuishi, JP Lewis, DR Demuth, RJ Lamont
  • Journal Title: Molecular Microbiology 69 Pages: 1153-1164
  • Peer Reviewed
• [Journal Article] P.gingivalis accelerates gingival epithelial cell progression through the cell cycle (2008)
  • Author(s): M Kuboniwa, Y Hasegawa, S Mao, A Amano, S Shizukuishi, RJ Lamont, O Yilmaz
  • Journal Title: Microbes and Infection 10 Pages: 122-128
  • Peer Reviewed
• [Journal Article] P. gingivalis accelerates gingival epithelial cell progression through the cell cycle (2008)
  • Author(s): Masae Kuboniwa
  • Journal Title: Microbes and Infection 10 Pages: 122-128
  • Peer Reviewed
• [Journal Article] A Porphyromonas gingivalis tyrosine phosphate is a multifunctional regulator of virulence attributes (2008)
  • Author(s): Kazuhiko Maeda
  • Journal Title: Molecular Microbiology 69 Pages: 1153-1164
  • Peer Reviewed
• [Presentation] Identification of Porphyromonas gingivalis client proteins with Streptococcus oralis GAPDH (2010)
  • Author(s): K Maeda, M Kuboniwa, M Iwasaki, M Toe, E Hashino, H Nagata
  • Organizer: 第58回国際歯科研究学会日本部会総会・学術大会
  • Place of Presentation: Kitakyushu
  • Year and Date: 2010-11-21
• [Presentation] Identification of Porphyromonas gingivalis client proteins with Streptococcus oralis GAPDH (2010)
  • Author(s): Kazuhiko Maeda
  • Organizer: 第58回国際歯科研究学会日本部会総会・学術大会
  • Place of Presentation: 北九州
  • Year and Date: 2010-11-21
• [Presentation] Streptococcus oralis GAPDHから同定されたPorphyromonas gingivalis線毛に結合するペプチドによるバイオフィルム形成の阻害 (2010)
  • Author(s): 永田英樹
  • Organizer: 第59回日本口腔衛生学会総会学術賞受賞講演
  • Place of Presentation: 新潟
  • Year and Date: 2010-10-07
• [Presentation] Role of 4-aminobenzoate in interspecies communication (2010)
  • Author(s): M Kuboniwa, A Chawla, E Hashino, H Nagata, A Amano, M Hackett, RJ Lamont, S Shizukuishi
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona
  • Year and Date: 2010-07-15
• [Presentation] Effect of erythritol on periodontopathic biofilm development (2010)
  • Author(s): E Hashino, M Kuboniwa, M Iwasaki, Y Yamamoto, K Suematsu, H Cho, S Shizukuishi
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona
  • Year and Date: 2010-07-15
• [Presentation] GAPDH peptide inhibits P.gingivalis biofilm formation with oral bacteria (2010)
  • Author(s): H Nagata, M Iwasaki, E Hashino, M Toe, S Shizukuishi
  • Organizer: 88^<th> General Session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona
  • Year and Date: 2010-07-15
• [Presentation] GAPDH peptide inhibits P.gingivalis biofilm formation with oral bacteria (2010)
  • Author(s): Hideki Nagata
  • Organizer: 88th General Session and Exhibition of International Association for Dental Research
  • Place of Presentation: Barcelona(Spain)
  • Year and Date: 2010-07-15
• [Presentation] Effect of erythritol on periodontopathic biofilm development (2010)
  • Author(s): Ei Hashino
  • Organizer: 88th General Session and Exhibition of International Association for Dental Research
  • Place of Presentation: Barcelona(Spain)
  • Year and Date: 2010-07-15
• [Presentation] Role of 4-aminobenzoate in interspecies communication (2010)
  • Author(s): Masae Kuboniwa
  • Organizer: 88th General Session and Exhibition of International Association for Dental Research
  • Place of Presentation: Barcelona(Spain)
  • Year and Date: 2010-07-15
• [Presentation] エリスリトールが歯周病原性バイオフィルム構成細菌の代謝に及ぼす影響 (2010)
  • Author(s): 橋野恵衣、東江正裕、田中宗雄、久保庭雅恵
  • Organizer: 大阪大学歯学会第110回例会
  • Place of Presentation: 吹田
  • Year and Date: 2010-07-08
• [Presentation] エリスリトールが歯周病原性バイオフィルム構成細菌の代謝に及ぼす影響 (2010)
  • Author(s): 橋野恵衣
  • Organizer: 大阪大学歯学会 第110回例会
  • Place of Presentation: 吹田
  • Year and Date: 2010-07-08
• [Presentation] オクラポリサッカライドが歯周病原性バイオフィルム形成に及ぼす影響 (2010)
  • Author(s): 山本裕美子、久保庭雅恵、橋野恵衣
  • Organizer: 第21回近畿・中国・四国口腔衛生学会総会
  • Place of Presentation: 松江
  • Year and Date: 2010-06-20
• [Presentation] Role of 4-aminobenzoate in periodontopathic biofilm development (2010)
  • Author(s): M Kuboniwa, E Hashino, M Toe, M Iwasaki, Y Yamamoto, S Shizukuishi
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: Yokohama
• [Presentation] Role of 4-aminobenzoate in periodontopathic biofilm development (2010)
  • Author(s): Masae Kuboniwa
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜市
• [Presentation] デンタルバイオフィルム形成に及ぼすStreptococcusoralis GAPDHペプチドの影響 (2009)
  • Author(s): 岩崎未央、橋野恵衣、東江正裕、永田英樹、久保庭雅恵、雫石聰
  • Organizer: 第20回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 広島
  • Year and Date: 2009-06-20
• [Presentation] デンタルバイオフィルム形成に及ぼすStreptococcus oralis GAPDHペプチドの影響 (2009)
  • Author(s): 岩崎未央
  • Organizer: 第20回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 広島市
  • Year and Date: 2009-06-20
• [Presentation] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): M Kuboniwa, EL Hendrickson, H Xia, E Hashino, S Shizukuishi, M Hackett, RJ Lamont
  • Organizer: The 2009 Gordon research Conference on Periodontal Disease
  • Place of Presentation: Colby-Sawyer College
• [Presentation] Streptococcus oralis GAPDH peptide inhibits Porphyromonas gingivalis biofilm formation (2009)
  • Author(s): H Nagata, M Iwasaki, M Kuboniwa, K Maeda, S Sekine, S Shizukuishi
  • Organizer: 87^<th> General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Miami
• [Presentation] Streptococcus oralis GAPDH peptide inhibits Porphyromonas gingivalis biofilm formation (2009)
  • Author(s): Hideki Nagata
  • Organizer: 87^<th> General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Miami, FL, USA
• [Presentation] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): Masae Kuboniwa
  • Organizer: The 2009 Gordon Research Conference on Periodontal Disease
  • Place of Presentation: Colby-Sawyer College, NH, USA
• [Presentation] Porphyromonas gingivalis線毛への口腔レンサ球菌菌体表層glyceraldehyde-3-phosphate dehydrogenaseの結合部位の解析 (2008)
  • Author(s): 岩崎未央、永田英樹、前田和彦、久保庭雅恵、橋野恵衣、雫石聰
  • Organizer: 大阪大学歯学会第106回例会
  • Place of Presentation: 吹田
  • Year and Date: 2008-07-17
• [Presentation] Porphyromonas gingivalis線毛への口腔レンサ球菌菌体表層glyceraldehyde-3-phosphate dehydrogenaseの結合部位の解析 (2008)
  • Author(s): 岩崎 未央
  • Organizer: 大阪大学歯学会第106回例会
  • Place of Presentation: 吹田市
  • Year and Date: 2008-07-17
• [Presentation] Distinct roles of major/minor fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis (2008)
  • Author(s): M Kuboniwa, A Amano, E Hashino, S Shizukuishi
  • Organizer: Biofilms III : 3^<rd> International Conference
  • Place of Presentation: Munich
• [Presentation] Porphyromonas gingivalisのバイオフィルム形成に及ぼすGAPDHペプチドの阻害効果 (2008)
  • Author(s): 岩崎未央、永田英樹、久保庭雅恵、前田和彦、橋野恵衣、雫石聰
  • Organizer: 第57回日本口腔衛生学会・総会
  • Place of Presentation: さいたま
• [Presentation] Role of Streptococcus gordonii Cbe protein in periodontopathic biofilm development (2008)
  • Author(s): M Kuboniwa, E Hashino, Y Yamamoto, RJ Lamont, S Shizukuishi
  • Organizer: 86^<th> General session & exhibition of the International Association for Dental Research
  • Place of Presentation: Toronto
• [Presentation] Role of streptococcus gordonii Cbe protein in periodontopathicbiofilm development (2008)
  • Author(s): Masae Kuboniwa
  • Organizer: 86^<th> General session & exhibition of theInternational Association for Dental Research
  • Place of Presentation: Toronto
• [Presentation] Porphyromonas gingivalisのバイオフィルム形成に及ぼすGAPDHペプチドの阻害効果 (2008)
  • Author(s): 岩崎 未央
  • Organizer: 第57回日本口腔衛生学会・総会
  • Place of Presentation: さいたま市
• [Presentation] Distinct roles of major/minor fimbriae and gingipains in homotypicbiofilm development by Porphyromonas gingivalis (2008)
  • Author(s): Masae Kuboniwa
  • Organizer: Biofilms III : 3^<rd> International Conference
  • Place of Presentation: Munich