| Project/Area Number |
20390538
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
YAEGAKI Ken The Nippon Dental University, 生命歯学部, 教授 (40166468)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAHARA Taka 日本歯科大学, 生命歯学部, 教授 (10366768)
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| Research Collaborator |
CALENIC B. 日本歯科大学, 大学院・生命歯学研究科, 学生
ISHKITIEV N. 日本歯科大学, 大学院・生命歯学研究科, 学生
伊井 久貴 日本歯科大学, 大学院・生命歯学研究科, 学生
青山 いずみ 日本歯科大学, 大学院・生命歯学研究科, 学生
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 歯周炎 / 幹細胞 / 肝細胞 / 硫化水素 / 口臭皮 / 活性酸素 / 肝臓様細胞 / 再生医療 / 口臭 / アポトーシス / ROS / 胚細胞 / 上皮細胞 / 歯髄 |
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| Research Abstract |
Stem cells were isolated from human deciduous and wisdom teeth using CD117 antibody and magnetic separation. The cells were passaged using the magnetic separation using CD117 antibody at every 4 passages. Hepatic and Pancreatic cells were differentiated from the cells, also differentiated using serum free media. The cells were dedifferentiated under 0.1ng/10mL hydrogen sulfide. Hepatic differentiation was accelerated, since hepatic markers expressed more than in control, and as urea production was increased. But pancreatic was suppressed as insulin production was suppressed. The pulp stem cells themselves caused strong apoptosis under hydrogen sulfide exposure.
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