Epidemiological study on mosquito-borne flavivirus infection inGuangdong Province, China

• Project/Area Number: 20405040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: Kyoto Sangyo University (2011-2012); Hokkaido University (2008-2010)
• Principal Investigator: MAEDA Akihiko 京都産業大学, 総合生命科学部, 教授 (70333359)
• Co-Investigator(Kenkyū-buntansha): MAEDA Junko 北海道大学, 大学院・獣医学研究科, 客員研究員 (70399989)
• Research Collaborator: KE Chang-wen 中国・広東省, CDC, 所長 ; JIANG Shu 中国・広東省, CDC, 研究員
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 蚊媒介性感染症 / フラビウイルス / 中国 / 広東省 / 疫学調査 / 日本脳炎ウイルス / デングウイルス / ウエストナイルウイルス / 鑑別試験法 / 日本脳疫ウイルス

Research Abstract

The outbreak of mosquito-borne flavivirus infection caused by recent world-globalization and global-warming, is one of big concerns on world public health. In this study, we developed a new protocol of differential serodiagnosis for flavivirus infection using molecular biological techniques. And we performed an epidemiological study on flavivirus infection in Guangdong province, China. We also analyzed the interrelation of an outbreak of flavivirus infection and meteorological conditions in this province.

Research Products

• [Journal Article] Review of diagnostic plaque reduction neutralization tests for flavivirus infection (2013)
  • Author(s): Maeda, A., and Maeda, J.
  • Journal Title: Vet. J. Volume: 195 Issue: 1 Pages: 33-40
  • DOI: 10.1016/j.tvjl.2012.08.019
  • Peer Reviewed
• [Journal Article] The chicken 2-5 oligoadenylate synthetase A inhibits the replication of West Nile Virus. (2012)
  • Author(s): Tag-El-din-Hassan
  • Journal Title: Jap. J. Vet. Res. Volume: 60 Pages: 95-103
  • Peer Reviewed
• [Journal Article] Development and Application of West Nile Virus Subgenomic Replicon RNA Expressing Secreted Alkaline Phosphatase (2011)
  • Author(s): Moritoh, K., Maeda, A., Sasaki, N., Agui, T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Issue: 5 Pages: 683-686
  • DOI: 10.1292/jvms.10-0495
  • NAID: 130000444749
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Deveropement and application of West Nile virus subgenomic replicon RNA expressing secreted alkarine phosphatase. (2011)
  • Author(s): Moritoh, K., Maeda, A., Sasaki, N., Agui, T.
  • Journal Title: J.Vet.Med.Sci. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Deveropement and application of West Nile virus subgenomic replicon RNA expressing secreted alkarine phosphatase. (2010)
  • Author(s): Moritoh, K., Maeda, A., Sasaki, N., Agui, T.
  • Journal Title: J.Vet.Med.Sci. In press
  • Peer Reviewed
• [Journal Article] Glycosylation of the West Nile virus envelope protein increases in vitro and in vivo viral multiplication in birds. (2010)
  • Author(s): Murata, R., Eshita, Y., Maeda, A., Maeda, J., Akita, S., Tanaka, T., Yoshii, K., Kariwa, H., Umemura, T., Takashima, I.
  • Journal Title: Am.J.Trop.Med.Hyg (印刷中)
  • Peer Reviewed
• [Journal Article] Molecular characterization of the E gene of dengue virus type 1 isolated in Guangdong Province, China, in 2006 (2009)
  • Author(s): Zheng, K., Zhou, H.-Q., Yan, J., Ke, C.-W. , Maeda, A., Maeda, J., Takashima, I., Kurane, I., Ma, H., and Xie, X.-M.
  • Journal Title: Epidem. Inf. Volume: 137 Issue: 1 Pages: 73-78
  • DOI: 10.1017/s0950268808000617
  • Peer Reviewed
• [Journal Article] A PCR-based protocol for generation of a recombinant West Nile virus. (2009)
  • Author(s): Maeda, A., Murata, R., Akiyama, M., Takashima, I., Kariwa, H., Watanabe, T., Kurane, I., Maeda, J.
  • Journal Title: Virus Res. 144 Pages: 35-43
  • Peer Reviewed
• [Journal Article] A PCR-based protocol for generation of a recombinant West Nile virus. (2009)
  • Author(s): Maeda, A., Murata, R., Akiyama, M., Takashima, L, Kariwa, H., Watanabe, T., Kurane, L, Maeda, J.
  • Journal Title: Virus Res. 144 Pages: 35-43
  • Peer Reviewed
• [Journal Article] 帯広市内で発見されたハシブトガラスCorvus macrohynchos白化個体死体のウイルス学的検査および剖検記録。 (2009)
  • Author(s): 吉田智生、藤本智、小林伸行、前田秋彦、前田潤子、大沼学、桑名貴、村田浩一、浅川満彦
  • Journal Title: 北獣会誌 53 Pages: 165-167
  • NAID: 120005761333
• [Journal Article] 北海道胆振地方にて採集されたカササギPica picaの剖検記録3例とウイルス学的検査 (2009)
  • Author(s): 吉田智生、上村純平、相澤空見子、盛田徹、吉田順子、前田秋彦、浅川満彦
  • Journal Title: 北獣会誌 53 Pages: 545-547
  • NAID: 120005761365
• [Journal Article] Molecular characterization of the E gene of dengue virus type 1 isolated in Guangdong Province, China, in 2006 (2009)
  • Author(s): Zheng, K., Zhou, H. -Q., Yan, J., Ke, C. -W., Maeda, A., Maeda, J., Takashima, I., Kurane, I., Ma, H., and Xie, X. -M.
  • Journal Title: Epidemiology and Infection 137 Pages: 73-78
  • Peer Reviewed
• [Journal Article] A PCR-based protocol for generating West Nile virus replicons (2008)
  • Author(s): Maeda, J., Takagi, H., Hashimoto, S., Kurane, I., and Maeda, A.
  • Journal Title: J. Virol. Methods Volume: 148 Issue: 1-2 Pages: 244-252
  • DOI: 10.1016/j.jviromet.2007.12.005
  • Peer Reviewed
• [Journal Article] A PCR-based protocol for generating West Nile virus replicons. (2008)
  • Author(s): Maeda, J., Takagi, H., Hashimoto, S., Kurane, I., Maeda, A.
  • Journal Title: J.Virol.Methods 148 Pages: 244-252
  • Peer Reviewed
• [Presentation] 京都市におけるアカイエカとチカイエカの捕集数および構成比の空間差異 (2013)
  • Author(s): 米島万有子
  • Organizer: 第65回日本衛生動物学会
  • Place of Presentation: 北海道，江別市
• [Presentation] 京都市における紅斑熱群リケッチアの検出. (2012)
  • Author(s): 染谷梓
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手県，盛岡市
• [Presentation] 中国・広東省の1978-2010年におけるデングウイルス感染症の流行調査 (2011)
  • Author(s): 前田秋彦、Chang-Wen,Ke,JiangShu,倉根一郎
  • Organizer: 第18回トガ・フラビ・ぺスチウイルス研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-11
• [Presentation] 中国・広東省の1978-2010年におけるデングウイルス感染症の流行調査 (2011)
  • Author(s): 前田秋彦、Chang-Wen, Ke, Jiang Shu, 倉根一郎
  • Organizer: 第18回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 国立感染症研究所(東京)
  • Year and Date: 2011-11-11
• [Presentation] ウエストナイルウイルスのウイルス様粒子作製法の改良 (2011)
  • Author(s): 前田秋彦、前田潤子、染谷梓、西野佳以、村田英雄、Velado Fernandez, Igor、倉根一郎
  • Organizer: 第152回日本獣医学界
  • Place of Presentation: 大阪府立大学(大阪)
  • Year and Date: 2011-09-17
• [Presentation] Epidemiological study of mosquito-borne diseases in Guangdong province, China. (2010)
  • Author(s): Maeda, A., Ke, Chang-Wen.
  • Organizer: The Workshop of Network Laboratories on Emergency Response and Surveillunce of Infectious Diseases in Pan Pearl River Delta Region
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2010-12-12
• [Presentation] Epidemiological study of mosquito-borne diseases in Guangdong rovince, China. (2010)
  • Author(s): Maeda, A., Ke, ChangWen
  • Organizer: The Workshop of Network Laboratories on Emergency Response and Surveillunce of Infectious Diseases in Pan Pearl River Delta Region
  • Place of Presentation: Guangdong CDC, Guangzhou, China
  • Year and Date: 2010-12-12
• [Presentation] ウエストナイルウイルス・レプリコンの持続的複製細胞株の樹立 (2010)
  • Author(s): 前田秋彦、染谷梓、西野佳以、村田英雄、倉根一郎
  • Organizer: トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-10
• [Presentation] ウエストナイルウイルス・レプリコンの持続的複製細胞株の樹立 (2010)
  • Author(s): 前田秋彦、染谷梓、西野佳以、村田英雄、倉根一郎
  • Organizer: トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 国立感染症研究所 (東京)
  • Year and Date: 2010-12-10
• [Presentation] Epidemiological study of mosquito-borne diseases in Guangdong province, China (2010)
  • Author(s): Maeda, A., and Ke, ChangWen
  • Organizer: WHO meeting
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2010-10-12
• [Presentation] ウエストナイルウイルスと日本脳炎ウイルスの鑑別中和試験法の開発 (2009)
  • Author(s): 前田秋彦、前田潤子、村田亮、白藤浩明、金平克史、苅和宏明、高島郁夫、倉根一郎
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-26
• [Presentation] ウエストナイルウイルスと日本脳炎ウイルスの鑑別中和試験法の開発 (2009)
  • Author(s): 前田秋彦、前田潤子、村田亮、白藤浩明、金平克史、苅和宏明、高島郁夫、倉根一郎
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 都市センターホテル(東京都千代田区)
  • Year and Date: 2009-10-26
• [Presentation] PCR法を用いた組み換えウエストナイルウイルスの作製 (2009)
  • Author(s): 秋山稔、村田亮、高島郁夫、苅和宏明、渡辺智正、倉根一郎、前田潤子、前田秋彦
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-26
• [Presentation] PCR法を用いた組み換えウエストナイルウイルスの作製 (2009)
  • Author(s): 秋山稔、村田亮、高島郁夫、苅和宏明、渡辺智正、倉根一郎、前田潤子、前田秋彦
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取市民会館(鳥取県鳥取市)
  • Year and Date: 2009-09-26
• [Presentation] PCR法による組み換えウエストナイルウイルスの作製 (2009)
  • Author(s): 秋山稔、村田亮、高島郁夫、苅和宏明、渡辺智正、倉根一郎、前田潤子、前田秋彦
  • Organizer: 第44回日本脳炎ウイルス生態研究会
  • Place of Presentation: 丸駒温泉旅館(北海道千歳市)
  • Year and Date: 2009-06-19
• [Presentation] ウエストナイルウイルスRNA複製の解析-マイナス鎖small RNAを中心に (2008)
  • Author(s): 前田秋彦、前田潤子、倉根一郎
  • Organizer: 第56回日本ウイルス学会
  • Place of Presentation: 岡山(岡山コンベンションセンター)
  • Year and Date: 2008-10-26
• [Presentation] ウイルス様粒子を用いたウエストナイルウイルスと日本脳炎ウイルス感染症との鑑別中和試験法の開発 (2008)
  • Author(s): 前田秋彦、前田潤子、村田亮、苅和宏明、高島郁夫、倉根一郎
  • Organizer: トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 岡山(岡山コンベンションセンター)
  • Year and Date: 2008-10-25
• [Presentation] ウエストナイルウイルスと日本脳炎ウイルスの鑑別中和試験法の開発 (2008)
  • Author(s): 前田潤子、村田亮、苅和宏明、倉根一郎、高島郁夫、前田秋彦
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-09-24
• [Presentation] ウエストナイルウイルスと日本脳炎ウイルスの鑑別中和試験法の開発 (2008)
  • Author(s): 前田潤子、村田亮、苅和宏明、倉根一郎、高島郁夫、前田秋彦
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎(シーガイア)
  • Year and Date: 2008-09-24
• [Presentation] ウエストナイルウイルスのウイルス様粒子の作製 (2008)
  • Author(s): 前田秋彦、前田潤子、橋本新吾苅和宏明、高島郁夫、倉根一郎
  • Organizer: 第43回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 香川(観音寺市、琴弾荘)
  • Year and Date: 2008-05-31
• [Presentation] Epidemiological study on Flaviviruses in Guangdong province, China (2008)
  • Author(s): Maeda A, Maeda J, Ma H, Ke, C -W, Takagi H, Takashima I, and Kurane I
  • Organizer: 42nd Joint Viral Diseases Panels Meeting, US-Japan Cooperative Medical Science Program
  • Place of Presentation: Nagasaki (Nagasaki University)
  • Year and Date: 2008-05-28
• [Presentation] Epidemiological study on Flaviviruses in Guangdong province, China (2008)
  • Author(s): Maeda, A., Maeda, J., Ma, H., Ke, C.-W., Takagi, H., Takashima, I., Kurane, I.
  • Organizer: 42nd Joint Viral Diseases Panels Meeting, US-Japan Cooperative Medical Science Program
  • Place of Presentation: Nagasaki
  • Year and Date: 2008-05-02
• [Book] Epidemiological study on Flaviviruses in Guangdong province, China,2005-2007. (2010)
  • Author(s): Ma, H., Ke, C.-W., Maeda, J., Takashima,I., Kurane, I., and Maeda, A.
  • Publisher: Ed. Maeda, A., RESEARCH SIGNPOST, India
• [Book] In Animal Viruses.("Differential sero-diagnosis of flaviviruses using sub-viral particles and virus-like particles")(Eds.Maeda.A.) (2010)
  • Author(s): Maeda, A., Maeda, J., Murata, R., Akiyama, M., Kariwa, H., Takashima, I., Kurane, I.
  • Publisher: Trans Research Network, Kerala, India
• [Book] In Animal Viruses.("Epidemiological study on Flaviviruses in Guangdong province, China, 2005-2007")(Eds.Maeda.A.) (2010)
  • Author(s): Ma, H., Ke, C.-W., Maeda, J., Takashima, I., Kurane, I., Maeda, A.
  • Publisher: Trans Research Network, Kerala, India
• [Book] Animal Viruses (2010)
  • Author(s): Maeda, A., Maeda, J., Murata, R., Akiyama, M., Kariwa, H., Takashima, I., Kurane, I.
  • Total Pages: 13
  • Publisher: Trans Research Network, Kerala, India
• [Book] Animal Viruses (2010)
  • Author(s): Ma, H., Ke, C.-W., Maeda, J., Takashima, I., Kurane, I., Maeda, A.
  • Total Pages: 14
  • Publisher: Trans Research Network, Kerala, India