The study on mycotoxin detoxification and protection of animal mycotoxicoses in Thaoland.

• Project/Area Number: 20405042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: KUMAGAI Susumu The University of Tokyo, 大学院・農学生命科学研究科, 教授 (60109965)
• Co-Investigator(Kenkyū-buntansha): KONISHI Yoshiko 国立医薬品食品衛生研究所, 衛生微生物部, 部長 (10195761) ; SAKUDA Shohei 東京大学, 大学院・農学生命科学研究科, 准教授 (80192087)
• Co-Investigator(Renkei-kenkyūsha): TAKATORI Kousuke NPO法人カビ相談センター, 理事長 ; PRAPEUK Tangmunkhong Kasetsart University, Faculty of Veterinary Medicine, Associate Professor ; AMNART Poapolathep Kasetsart University, Faculty of Veterinary Medicine, Associate Professor
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: アフラトキシン / ゼアラレノン / タイ / 家畜 / 分解

Research Abstract

In order to find metabolic detoxification by micro-organisms in soil of Thailand, where heavy contamination with mycotoxins has been observed, bacteria and fungi isolated from Cassava farms in Thailand were examined for their activities to metabolize aflatoxin, ochratoxin A and zearalenone. The result demonstrated some bacteria and fungi to metabolize aflatoxin B1 and ochratoxin A. Feces of horse, sheep and pig living in the central region of Thailand, and anaerobic bacteria isolated from the feces, were also examined for their metabolic activities toward mycotoxins, but clear metabolism was not found. With regard to animal tissues, a possible conversion of aflatoxin B2 to B1 by the liver tissues from various animals was suggested. Also tissues of various organs of goat were demonstrated to metabolize zearalenone to alpha-and beta-zearalenols.

Research Products

• [Journal Article] Simulation of deoxynivalenol intake from wheat consumption in Japan using the Monte Carlo method. (2011)
  • Author(s): Nakatani Y, Satoh T, Saito S, Watanabe M, Yoshiike N, Kumagai S, Sugita-Konishi Y
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 28(4) Pages: 471-6
  • Peer Reviewed
• [Journal Article] Rapid deposition of glomerular IgA in BALB/c mice by nivalenol and its modifying effect on high IgA strain (HIGA) mice. (2011)
  • Author(s): Dewa Y, Kemmochi S, Kawai M, Saegusa Y, Harada T, Shimamoto K, Mitsumori K, Kumagai S, Sugita-Konishi Y, Shibutani M
  • Journal Title: Exp Toxicol Pathol. 63(1-2) Pages: 17-24
  • Peer Reviewed
• [Journal Article] Enumeration of Fungi in Fruits by the Most Probable Number Method. (2010)
  • Author(s): Watanabe M, Tsutsumi F, Lee KI, Sugita-Konishi Y, Kumagai S, Takatori K, Hara-Kudo Y, Konuma H
  • Journal Title: J Food Sci. 75(9)
  • Peer Reviewed
• [Journal Article] Rapid and effective DNA extraction method with bead grinding for a large amount of fungal DNA. (2010)
  • Author(s): Watanabe M, Lee K, Goto K, Kumagai S, Sugita-Konishi Y, Hara-Kudo Y
  • Journal Title: J Food Prot. 73(6) Pages: 1077-84
  • Peer Reviewed
• [Journal Article] The effect of naringenin on the fate and disposition of deoxynivalenol in piglets. (2010)
  • Author(s): Poapolathep A, Poapolathep S, Sugita-Konishi Y, Wongpanit K, Machii K, Itoh Y, Kumagai S
  • Journal Title: J Vet Med Sci. 72(10) Pages: 1289-94
  • NAID: 130000264215
  • Peer Reviewed
• [Journal Article] Exposure to aflatoxins in Japan : risk assessment for aflatoxin B1. (2010)
  • Author(s): Sugita-Konishi Y, Sato T, Saito S, Nakajima M, Tabata S, Tanaka T, Norizuki H, Itoh Y, Kai S, Sugiyama K, Kamata Y, Yoshiike N, Kumagai S
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 27(3) Pages: 365-72
  • Peer Reviewed
• [Journal Article] Four-year surveillance for ochratoxin a and fumonisins in retail foods in Japan. (2010)
  • Author(s): Aoyama K, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Fujita K, Kai S, Tsutsumi T, Takahashi M, Tanaka H, Iizuka S, Ogiso M, Maeda M, Yamaguchi S, Sugiyama K, Sugita-Konishi Y, Kumagai S.
  • Journal Title: J Food Prot. 73(2) Pages: 344-52
  • Peer Reviewed
• [Journal Article] Metabolic conversion of zearalenone to alpha-zearalenol by goat tissues. (2010)
  • Author(s): Dong M, Tulayakul P, Li JY, Dong KS, Manabe N, Kumagai S.
  • Journal Title: J Vet Med Sci. 72(3) Pages: 307-12
  • NAID: 130000134546
  • Peer Reviewed
• [Journal Article] The toxic effects and fate of intravenously administered zearalenone in goats. (2010)
  • Author(s): Dong M, He XJ, Tulayakul P, Li JY, Dong KS, Manabe N, Nakayama H, Kumagai S
  • Journal Title: Toxicon. 55(2-3) Pages: 523-30
  • Peer Reviewed
• [Journal Article] The effect of naringenin on the fate and disposition of deoxynivalenol in piglets. (2010)
  • Author(s): Poapolathep A, Poapolathep S, Sugita-Konishi Y, Wongpanit K, Machii K, Itoh Y, Kumagai S.
  • Journal Title: J Vet Med Sci Volume: 72 Pages: 1289-1294
  • NAID: 130000264215
  • Peer Reviewed
• [Journal Article] Exposure to aflatoxins in Japan : risk assessment for aflatoxin Bl (2010)
  • Author(s): Sugita-Konishi Y, Sato T, Saito S, Nakajima M, Tabata S, Tanaka T, Norizuki H, Itoh Y, Kai S, Sugiyama K, Kamata Y, Yoshiike N, Kumagai S.
  • Journal Title: Food Addit Contam Part A Volume: 27 Pages: 365-372
  • Peer Reviewed
• [Journal Article] Rapid and effective DNA extraction method with bead grinding for a large amount of fungal DNA. (2010)
  • Author(s): Watanabe M, Lee K, Goto K, Kumagai S, Sugita-Konishi Y, Hara-Kudo Y.
  • Journal Title: J Food Prot Volume: 73 Pages: 1077-1084
  • Peer Reviewed
• [Journal Article] Four-year surveillance for ochratoxin a and fumonisins in retail foods in Japan. (2010)
  • Author(s): Aoyama K, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizu ki H, Itoh Y, Fujita K, Kai S, Tsutsumi T, Takahashi M, Tanaka H, Iizuka S, Ogiso M, Maeda M, Yamaguchi S, Sugiyama K, Sugita-Konishi Y, Kumagai S
  • Journal Title: J Food Prot. 73 Pages: 344-52
  • Peer Reviewed
• [Journal Article] The toxic effects and fate of intravenously administered zearalenone in goats (2010)
  • Author(s): Dong M, He XJ, Tulayakul P, Li JY, Dong KS, Manabe N, Nakayama H, Kumagai S.
  • Journal Title: Toxicon 55 Pages: 523-30
  • Peer Reviewed
• [Journal Article] Metabolic conversion of zearalenone to α-zearalenol by goat tissues. (2010)
  • Author(s): Mei Dong, Phitsanu Tulayakul, Jun You Li, KeSu Dong, Noboru Manabe, Susumu Kumagai.
  • Journal Title: J.V.M.S. 72 Pages: 307-12
  • Peer Reviewed
• [Journal Article] Exposure to aflatoxins in Japan : risk assessment for aflatoxin B1. (2010)
  • Author(s): Y.Sugita-Konishia, T.Satob, S.Saitob, M.Nakajimac, S.Tabatad, T.Tanakae, H.Norizukif, Y.Itoha, S.Kaig, K.Sugiyamaa, Y.Kamataa, N.Yoshiikeh, S.Kumagai.
  • Journal Title: Food Addit Contam. 27 Pages: 365-72
  • Peer Reviewed
• [Journal Article] Determination of a yellow rice toxin, luteoskyrin, in rice by using liquid chromatography-tandem mass spectrometry with electrospray ionization. (2009)
  • Author(s): Mizutani K, Kumagai S, Mochizuki N, Kitagawa Y, Sugita-Konishi Y.
  • Journal Title: J Food Prot. 72(6) Pages: 1321-6
  • Peer Reviewed
• [Journal Article] Dispositions and residue depletion of enrofloxacin and its metabolite ciprofloxacin in muscle tissue of giant freshwater prawns(Macrobrachium rosenbergii). (2009)
  • Author(s): Poapolathep A, Jermnak U, Chareonsan A, Sakulthaew C, Klangkaew N, Sukasem T, Kumagai S.
  • Journal Title: J Vet Pharmacol Ther. 32 Pages: 229-34
  • Peer Reviewed
• [Journal Article] Determination of a yellow rice toxin, luteoskyrin, in rice by using liquid chromatography-tandem mass spectrometry with electrospray ionization. (2009)
  • Author(s): Mizutani K, Kumagai S, Mochizuki N, Kitagawa Y, Sugita-Konishi Y.
  • Journal Title: J Food Prot. 72 Pages: 1321-6
  • Peer Reviewed
• [Journal Article] Aflatoxin and ochratoxin A contamination of retail foods and intake of these mycotoxins in Japan. (2008)
  • Author(s): Kumagai S, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Aoyama K, Fujita K, Kai S, Sato T, Saito S, Yoshiike N, Sugita-Konishi Y
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 25(9) Pages: 1101-6
  • Peer Reviewed
• [Journal Article] Detection of deoxynivalenol contamination in wheat products in Thailand. (2008)
  • Author(s): Poapolathep A, Poapolathep S, Klangkaew N, Sugita-Konishi Y, Kumagai S
  • Journal Title: J Food Prot. 71(9) Pages: 1931-3
  • Peer Reviewed
• [Journal Article] Aflatoxin and ochratoxin A contamination of retail foods and intake of these mycotoxins in Japan. (2008)
  • Author(s): Kumagai S, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Aoyama K, Fujita K, Kai S, Sato T, Saito S, Yoshiike N, Sugita-Konishi Y.
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 25(9) Pages: 1101-6
  • Peer Reviewed
• [Journal Article] Fate of fusarenon-X in broilers and ducks. (2008)
  • Author(s): Poapolathep, A., Poapolathep, S., Sugita-Konishi Y., Imsilp, K., Tasanawat T., Sinthusing C., Itoh Y and Kumagai, S.
  • Journal Title: Poultry Sci. 187 Pages: 1510-15
  • Peer Reviewed
• [Journal Article] Detection of deoxynivalenol contamination in wheat products in Thailand. (2008)
  • Author(s): Poapolathep, A., Poapolathep, S., Klangkaew, N., Sugita-Konishi Y., and Kumagai, S.
  • Journal Title: J.Food Protection 71 Pages: 1931-33
  • Peer Reviewed
• [Journal Article] The effects of subqUtaneous administration of T-2 toxin on liver drug metabolizing enzymes in piglets. (2008)
  • Author(s): K. Dong, Y. Sugita-Konishi, J. Yu, P. Tulayakul, S. Kumagai.
  • Journal Title: Toxicological & Environmental Chemistry 90 Pages: 401-13
  • Peer Reviewed
• [Journal Article] Distribution and residue depletion of oxytetracycline in giant freshwater prawn (Macrobrachium rosenbergii). (2008)
  • Author(s): Poapolathep, A., Poapolathe P, S., Imsilp, K., Wannapat, N., Klangkaew, N., Kusutjarit, N. and Kumagai, S.
  • Journal Title: Journal of Food Protection 70 Pages: 870-73
  • Peer Reviewed
• [Journal Article] A 90-day subchronic toxicity study of nivalenol, a trichothecene mycotoxin, in F344 rats. (2008)
  • Author(s): Takahashi, M., Shibutani, M., Sugita-Konishi, Y., Aihara, M., Inoue, K., Woo, G-H., Fujimoto, H., Hirose, M.
  • Journal Title: Food and Chemical Toxicology 46 Pages: 125-35
  • Peer Reviewed
• [Journal Article] Immunotoxicity of Nivalenol after Subchronic Dietary Exposure to Rats. (2008)
  • Author(s): Kubosaki, A., Aihara, M., Park, B-J., Sugiura, Y., Shibutani,, M., Hirose, M., Suzuki, Y., Takatori, K., Sugita-Konishi, Y.
  • Journal Title: Food and Chemical Toxicology 46 Pages: 253-58
  • Peer Reviewed
• [Journal Article] Spiroethers of German chamomile inhibit production of aflatoxin G1 and trichothecene mycotoxin by inhibiting cytochrome P450 monooxygenases involved in their biosynthesis (2008)
  • Author(s): Yoshinari, T.; Yaguchi, A.; Takahashir-Ando, N.; Kimura, M.; Takahashi, H; Nakajima, T., Sugita-Konishi, Y.; Nagasawa, H.; Sakuda, S.
  • Journal Title: FEMS Microbiology Letters 284 Pages: 1884-90
  • Peer Reviewed
• [Journal Article] Determination of Organothins in human breast milk by gas chromatography with flame photometric detection. (2008)
  • Author(s): Mino, Y., Amano, F., Yoshioka, T., Konishi, Y.
  • Journal Title: J.Health Science 54 Pages: 224-28
  • Peer Reviewed
• [Journal Article] Nivalenol targets female reproductive system as well as hematopoietic and immune systems in rats after 90-day exposure through diet. (2008)
  • Author(s): Y. Sugita-Konishi,, A. Kubosaki, M. Aihara, B. J. Park, T. Tanaka, Y. Suzuki, K. Takatori, M. Hirose, M. Shibutani,
  • Journal Title: Food additives and contamination 25 Pages: 1118-27
  • Peer Reviewed
• [Journal Article] Surveillance of mycotoxin contamination in retail foods and exposure assessment based on it in Japan. (2008)
  • Author(s): Kumagai S. 1, Nakajima M., Tabata S., , Tanaka T., Norizuki H., Itoh Y., Sato T., Saito S., Yoshiike N., Takatori K. 11, Sugita-Konishi Y
  • Journal Title: Food additives and contamination 25 Pages: 1101-6
  • Peer Reviewed
• [Journal Article] Structural and conformational analysis of hydroxycyclovchlorotine and cyclochlorotine, chlorinated cyclic peptides from Penicillum islandicum. (2008)
  • Author(s): Mizutani, K., Hirasawa, Y., Sugita-Konishi, Y., Mochizuki, N., Morita, H.
  • Journal Title: J. Nat. Prod. 71 Pages: 1297-300
  • Peer Reviewed
• [Presentation] The effect of neonatal genistein exposure on aflatoxin B1 metabolism and toxicity in rats (2010)
  • Author(s): Poapolathep, S.Poapolathep, A., Machii, K., Nakayama, H., Sugita-Konishi, Y.Kumagai, S.
  • Organizer: The 49th Annual Meeting of the Society of Toxicology
  • Place of Presentation: ソルトレーク市(米国)
  • Year and Date: 2010-03-08
• [Presentation] Species comparison of in vitro metabolic conversion of aflatoxin B2 to aflatoxin B1. (2010)
  • Author(s): Poapolathep A., Poapolathep, S., Isariyodom, S., Imsilp, K., Klangkaew, N., Sugita-Konishi, Y., Kumagai, S.
  • Organizer: The 49th Annual Meeting of the Society of Toxicology
  • Place of Presentation: ソルトレーク市(米国)
  • Year and Date: 2010-03-08
• [Presentation] 動物体内におけるカビ毒の挙動に関する研究 (2009)
  • Author(s): 熊谷進
  • Organizer: 日本マイコトキシン学会学術講演会
  • Place of Presentation: 千葉大学
  • Year and Date: 2009-08-28
• [Book] 食の安全科学の展開 (2010)
  • Author(s): 熊谷進, 他40名
  • Total Pages: 287
  • Publisher: シーエムシー出版