Population genetics analysis of Plasmodium vivax-infected erythrocyte surface protein PvSTP

• Project/Area Number: 20406009
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: KANEKO Osamu Nagasaki University, 熱帯医学研究所, 教授 (50325370)
• Research Collaborator: JETSUMON Sattabongkot タイ王国在タイ米国陸軍医学研究所, 室長 ; KESINEE Chotivanich タイ王国マヒドン大学, 熱帯医学部, 准教授 ; 曹 雅明 中国医科大学, 医学部, 教授 ; MARCELO Urbano Ferreira ブラジル・サンパウロ大学, 医科学研究所, 教授
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 原虫 / 感染症 / 寄生虫 / 遺伝子多型 / 抗原変異

Research Abstract

Plasmodium vivax PvSTP2 was detected more abundantly than PvSTP1 at the transcription and translation level. PvSTP2 was located on the parasite-infected erythrocyte surface and colocalized with a Schuffner's dots, dot-like structure observed beneath the parasite-infected erythrocyte membrane. Positive diversifying selection was detected on PvSTP1 gene locus and many Vivax malaria patients possessed antibodies against PvSTP1 or PvSTP2.

Research Products

• [Presentation] Antibody Responses to Plasmodium vivax Subtelomeric Transmembrane Protein (PvSTP), a Homolog of Plasmodium falciparum SURFIN, in P.vivax-infected Patients (2011)
  • Author(s): Sungkapong T, Yahata K, Culleton R, Tsuboi T, Torii M, Ruengveerayuth R, Sattabongkot J, Udomsangpetch R, Kaneko O, Chotivanich K
  • Organizer: The Royal Golden Jubilee-Ph.D.program Congress XII
  • Place of Presentation: パタヤ、タイ
• [Presentation] Antibody response to Plasmodium vivax Subtelomeric Transmembrane Protein (PvSTP), a homolog of Plasmodium falciparum SURFIN, in P. vivax-infected patients (2010)
  • Author(s): Sungkapong T, Yahata K, Culleton R, Tsuboi T, Ruengveerayuth R, Ruengveerayuth R, Sattabongkot J, Udomsangpetch R, Kaneko O, Chotivanich K.
  • Organizer: Joint International Tropical Medicine Meeting 2010 and International Malaria Colloquium 2010
  • Place of Presentation: バンコク、タイ
• [Presentation] Plasmodium vivax subtelomeric transmembrane protein 2 (PvSTP2), a homolog of P. falciparum SURFIN : transcription and localization (2010)
  • Author(s): 金子修
  • Organizer: XII Brazilian Meeting on Malaria Research
  • Place of Presentation: オウロプエルト、ブラジル
• [Presentation] 三日熱マラリア原虫PvSTPの解析 (2010)
  • Author(s): Sungkapong T,矢幡一英,Chotivanch K,金子修
  • Organizer: 第18回分子寄生虫学
  • Place of Presentation: ワークショップ、草津
• [Presentation] Plasmodium SURFIN : evolution, polymorphism, and human sera reactivity (2010)
  • Author(s): 金子修
  • Organizer: Nagasaki Singapore Symposium
  • Place of Presentation: シンガポール
• [Presentation] Plasmodium SURFIN : evolution, polymorphism, and human sera reactivity (2010)
  • Author(s): Kaneko O
  • Organizer: Nagasaki Singapore Symposium
  • Place of Presentation: シンガポール、シンガポール
• [Presentation] 三日熱マラリア原虫PvSTPの解析 (2010)
  • Author(s): Sungkapong T, 矢幡一英, Chotivanch K, 金子修
  • Organizer: 第18回分子寄生虫学ワークショップ
  • Place of Presentation: 群馬県、草津、草津セミナーハウス
• [Presentation] Plasmodium vivax subtelomeric transmembrane protein 2 (PvSTP2), a homolog of P.falciparum SURFIN : transcription and localization (2010)
  • Author(s): Kaneko O
  • Organizer: XII Brazilian Meeting on Malaria Research
  • Place of Presentation: オウロプエルト、ブラジル
• [Presentation] Antibody response to Plasmodium vivax Subtelomeric Transmembrane Prote in (PvSTP), a homolog of Plasmodium falciparum SURFIN, in P.vivax-infected patients (2010)
  • Author(s): Sungkapong T, Yahata K, Culleton R, Tsuboi T, Torii M, Ruengveerayuth R, Sattabongkot J, Udomsangpetch R, Kaneko O, Chotivanich K
  • Organizer: oint International Tropical Medicine Meeting 2010 and International Malaria Colloquium 2010
  • Place of Presentation: バンコク、タイ
• [Presentation] マラリアは世界的な問題だけど、科学はどこまで追い詰めてるの? (2009)
  • Author(s): 金子修
  • Organizer: 第27回日本国際保健医療学会西日本地方会
  • Place of Presentation: 大阪
  • Year and Date: 2009-02-28
• [Presentation] Plasmodium SURFIN : evolution, polymorphism, and human sera reactivity (2009)
  • Author(s): 金子修
  • Organizer: The 4th Nagasaki Symposium on Tropical and Emerging Infectious Diseases
  • Place of Presentation: 長崎
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN_<4.1> (2009)
  • Author(s): Xangsayarath P, Kaewthamasorn M, Yahata K, Nakazawa S, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 宜野湾市
• [Presentation] Polymorphism patterns of Plasmodium vivax subtelomeric transmembrane protein 1 (PvSTP1) among China field isolates. (2009)
  • Author(s): Zhu XT, Kaewthamasorn M, Yahata K, Cao YM, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 宜野湾市
• [Presentation] Diversity of Plasmodium falciparum SURFIN_<4.2> in Thai field isolates (2009)
  • Author(s): Kaewthamasorn M, Yahata Y, Alexandre JSF, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 宜野湾市
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN_<4.1> (2009)
  • Author(s): Xangsayarath P, Kaewthamasorn M, Yahata K, Nakazawa S, Kaneko O
  • Organizer: The 3rd National Health Research Forum to Support the health research systems strengthening in Lao PDR
  • Place of Presentation: ラオス
• [Presentation] マラリア原虫の赤血球侵入・感染赤血球表面の生物学 (2009)
  • Author(s): 金子修
  • Organizer: 第17回分子寄生虫学ワークショップ
  • Place of Presentation: 草津
• [Presentation] Plasmodium vivax subtelomeric transmembrane protein 1 (PvSTP1), a homolog of P. falciparum SURFIN ; polymorphism and human sera reactivity. (2009)
  • Author(s): Zhu XT, Sungkapong T, Kaewthamasorn M, Culleton R, Tachibana M, Tsuboi T, Torii M, Yahata K, Cao YM, Chotivanch K, Kaneko O
  • Organizer: Vivax Malaria Research III : 2009 and beyond
  • Place of Presentation: パナマ
• [Presentation] The diversity of the extracellular domains of Plasmodium falciparum SURFIN family proteins ; evidence for positive diversifying selection (2009)
  • Author(s): Tang J, Yahata K, Torii M, Kaneko O
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
• [Presentation] Characterization of Plasmodium vivax subtelomeric transmembrane protein (PvSTP), a homolog of P. falciparum SURFIN (2009)
  • Author(s): Sungkapong T, Culleton R, Yahata K, Tsuboi T, Torii M, Sattabongkot J, Kaneko O, Chotivanch K.
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN_<4.2> (2009)
  • Author(s): Kaewthamasorn M, Yahata Y, Alexandre JSF, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
• [Presentation] Plasmodium vivax subtelomeric transmembrane protein 1 (PvSTP1), a homolog of P. faiciparum SURFIN, is highly polymorphic (2009)
  • Author(s): Zhu XT, Kaewthamasorn M, Yahata K, Cao YM, Kaneko O
  • Organizer: 43rd Annual U.S.-japan joint Conference of Parasitic Diseases
  • Place of Presentation: 東京
• [Presentation] Plasmodium vivax subtelomeric transmembrane protein 1(PvSTP1), a homolog of P.falciparum SURFIN ; polymorphism and human sera reactivity. (2009)
  • Author(s): Zhu XT, Sungkapong T, Kaewthamasorn M, Culleton R, Tachibana M, Tsuboi T, Torii M, Yahata K, Cao YM, Chotivanch K, Kaneko O
  • Organizer: Vivax Malaria Research III : 2009 and beyond
  • Place of Presentation: パナマ、パナマ共和国
• [Presentation] マラリア原虫の赤血球侵入・感染赤血球表面の生物学 (2009)
  • Author(s): 金子修
  • Organizer: 第17回分子寄生虫学ワークショップ
  • Place of Presentation: 群馬県(草津)、草津セミナーハウス
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN4.1 (2009)
  • Author(s): Xangsayarath P, Kaewthamasorn M, Yahata K, Nakazawa S, Kaneko O.
  • Organizer: The 3rd National Health Research Forum to Support the health research systems strengthening in Lao PDR
  • Place of Presentation: チャンパーサック、ラオス
• [Presentation] Variation in the rhophl/clag multigene family of Plasmodium falciparum from Thai field isolates (2009)
  • Author(s): Alexandre JSF, Kaewthamasorn M, Yahata K, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 沖縄(宜野湾市)、沖縄コンベンションセンター
• [Presentation] Diversity of Plasmodium falciparum SURFIN4.2 in Thai field isolates (2009)
  • Author(s): Kaewthamasorn M, Yahata Y, Alexandre JSF, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 沖縄(宜野湾市)、沖縄コンベンションセンター
• [Presentation] Polymorphism patterns of Plasmodium vivax subtelomeric transmembrane protein 1(PvSTPl)among China field isolates (2009)
  • Author(s): Zhu XT, Kaewthamasorn M, YahataK, Cao YM, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 沖縄(宜野湾市)、沖縄コンベンションセンター
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN4.1 (2009)
  • Author(s): Xangsayarath P, Kaewthamasorn M, Yahata K, Nakazawa S, Kaneko O
  • Organizer: 第50回日本熱帯医学会大会
  • Place of Presentation: 沖縄(宜野湾市)、沖縄コンベンションセンター
• [Presentation] Plasmodium SURFIN : evolution, polymorphism, and human sera reactivity (2009)
  • Author(s): Kaneko O
  • Organizer: The 4th Nagasaki Symposium on Tropical and Emerging Infectious Diseases
  • Place of Presentation: 長崎、長崎大学良順会館
• [Presentation] Plasmodium vivax subtelomeric transmembrane protein 1 (PVSTP1), a homolog of P. falciparum SURFIN, is highly polymorphic (2009)
  • Author(s): Zhu XT, Kaewthamasorn M, Yahata K, Cao YM, Kaneko O
  • Organizer: 43rd Annual U. S. -Japan Joint Conference of Parasitic Diseases
  • Place of Presentation: 東京
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN4.2 (2009)
  • Author(s): Kaewthamasorn M, Yahata Y, Alexandre JSF, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
• [Presentation] マラリア原虫感染赤血球表面分子に対する選択圧 (2008)
  • Author(s): 金子修、Kaewthamasorn M、矢幡一英、Alexandre JSF、中澤秀介、鳥居本美、Sattabongkot J、 Udomsangpetch R
  • Organizer: 第49回日本熱帯医学会大会・第23回日本国際保健医療学会学術大会合同大会
  • Place of Presentation: 東京
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN_<4.2> (2008)
  • Author(s): Kaewthamasorn M, Yahata K, Alexandre JSF, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O
  • Organizer: XVIIth International Congress for Tropical Medicine and Malaria
  • Place of Presentation: 韓国
• [Presentation] マラリア原虫感染赤血球表面分子に対する選択圧 (2008)
  • Author(s): Kaewthamasorn M,矢幡一英、Alexandre JSF、中澤秀介、鳥居本美、Sattabongkot J、Udomsangpetch R、金子修
  • Organizer: 第16回分子寄生虫学ワークショップ
  • Place of Presentation: 草津
• [Presentation] Positive diversifying selection on Plasmodium falciparum SURFIN4.2 (2008)
  • Author(s): Kaewthamasorn M, Yahata K, Alexandre JSF, Nakazawa S, Torii M, Sattabongkot J, Udomsangpetch R, Kaneko O.
  • Organizer: XVIIth International Congress for Tropical Medicine and Malaria
  • Place of Presentation: 韓国