Molecular analyses of G6PD variants in malaria endemic areas in Asian and African countries

• Project/Area Number: 20406023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: MATSUOKA Hiroyuki Jichi Medical University, 医学部, 教授 (10173816)
• Co-Investigator(Kenkyū-buntansha): HIRAI Makoto 群馬大学, 医学部, 講師 (50326849) ; 佐野 元市郎 自治医科大学, 医学部, 助教 (30383730)
• Co-Investigator(Renkei-kenkyūsha): KAWAMOTO Fumihiko 大分大学, 医学部, 教授 (40115556) ; MATSUDA Hajime 獨協医科大学, 医学部, 名誉教授 (30114648)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: グルコース6リン酸脱水素酵素 / 先天性代謝異常症 / 溶血発作マラリア / プリマキン / 感染蚊対策 / 分子疫学 / グルコニス6リン酸脱水素酵素 / マラリア / 人類遺伝学 / 溶血性貧血 / 新生児黄疸 / 東南アジア / シエラレオネ

Research Abstract

We visited malaria endemic areas in Asian and African countries to introduce rapid methods for malaria diagnosis and glucose-6-phosphate dehydrogenase (G6PD) activity test. For malaria diagnosis, acridine orange method was employed. For G6PD activity test, WST-8 method was used. These methods were done at the site of malaria endemic areas and results were informed within 30 minutes after taking blood. Patients could receive anti-malarial medicine accordingly including primaquine to eliminate gametocytes. Primaquine was not used for the patients with G6PD deficiency. WST-8 method was useful for the detection of G6PD deficiency in rural areas without electricity and was recommendable for use in malaria control programs. We found more than 700 G6PD deficient people in these activities. DNA analysis of G6PD gene was performed after receiving informed consent. Thus we read about 400 samples and found 22 molecular variants including 5 new G6PD variants.

Research Products

• [Journal Article] 溶血発作で発症したグルコース6リン酸脱水素酵素異常症の早産児の1例 (2011)
  • Author(s): 赤澤陽平, 馬場淳, 伊藤有香子, 才田謙, 平林佳奈枝, 三澤由佳, 石田岳史, 服部幸夫, 藤井寿一, 川本文彦, 松岡裕之
  • Journal Title: 日本未熟児新生児学会雑誌 23(1) Pages: 108-112
  • NAID: 10029413972
• [Journal Article] A new glucose-6-phosphate dehydrogenase deficiency variant, G6PD Mizushima, showing increases in serum ferritin and cytosol leucine aminopeptidase levels. (2011)
  • Author(s): Suga Y, Nagita A, Takesako R, Tanaka I, Kobayashi K, Hirai M, Matsuoka H
  • Journal Title: J Pediatr Hematol Oncol 33(1) Pages: 15-17
• [Journal Article] 溶血発作で発症したグルコース6リン酸脱水素酵素異常症の早産児の1例 (2011)
  • Author(s): 赤澤陽平
  • Journal Title: 日本未熟児新生児学会雑誌 Volume: 23 Pages: 108-112
  • Peer Reviewed
• [Journal Article] Incidence and mutation analysis of glucose-6-phosphate dehydrogenase deficiency in eastern Indonesian populations. (2010)
  • Author(s): Tantular IS, Matsuoka H, Kasahara Y, Pusarawati S, Kanbe T, Tuda JSB, Kido Y, Dachlan YP, Kawamoto F.
  • Journal Title: Acta Med Okayama 64(6) Pages: 367-373
  • NAID: 120002690099
• [Journal Article] The first case of a class I glucose-6-phosphate dehydrogenase deficiency, G6PD Santiago de Cuba (1339 G>A), from Chinese population as found in a survey for G6PD deficiency in northeastern and central China. (2010)
  • Author(s): Wang J, Matsuoka H, Hirai M, Mu L, Yang L, Luo E.
  • Journal Title: Acta Med Okayama 64(1) Pages: 49-54
• [Journal Article] Incidence and mutation analysis of glucose-6-phosphate dehydrogenase deficiency in eastern Indonesian populations (2010)
  • Author(s): Tantular IS
  • Journal Title: Acta Medica Okayama Volume: 64 Pages: 367-373
  • Peer Reviewed
• [Journal Article] The first case of a class I glucose-6-phosphate dehydrogenase deficiency, G6PD Santiago de Cuba (1339 G>A), from Chinese population as found in a survey for G6PD deficiency in northeastern and central China (2010)
  • Author(s): Wang J
  • Journal Title: Acta Medica Okayama Volume: 64 Pages: 49-54
  • Peer Reviewed
• [Journal Article] Glucose-6-phosphate dehydrogenase (G6PD) variants in three minority ethnic groups in central and northern Vietnam. (2009)
  • Author(s): Hung NM, Matsuoka H, Eto H, Mita T, Tinh TT, Dao LM, Trung PH, Phuong VN, Marma, ASP, Kobayakawa T.
  • Journal Title: Trop Med Health 37(1) Pages: 17-20
  • NAID: 10025715046
• [Journal Article] Fatal Chromobacterium violaceum septicaemia in northern Laos, a modified oxidase test and post-mortem forensic family G6PD analysis. (2009)
  • Author(s): Slesak G, Douangdala P, Inthalad S, Silisouk J, Vongsouvath M, Senguangphachanh A, Moore CE, Mayxay M, Matsuoka H, Newton PN
  • Journal Title: Annals Clin Microbiol Antimicrobials 8 Pages: 24-24
• [Journal Article] T-cell epitope polymorphisms of the Plasmodium falciparum circumsporozoite protein among field isolates from Sierra Leone : age-dependent haplotype distribution? (2009)
  • Author(s): Jalloh A, Jalloh M, Matsuoka H
  • Journal Title: Malaria Journal 8 Pages: 120-120
  • Peer Reviewed
• [Journal Article] Polymorphism at the apical membrane antigen 1 gene(AMA1)of the malaria parasite Plasmodium falciparum in a Vietnamese population (2009)
  • Author(s): Quang ND, Hoa PTP, Tuan MS, Viet NX, Jalloh A, Matsuoka H
  • Journal Title: Biochemical Genetics 47 Pages: 370-383
  • Peer Reviewed
• [Journal Article] Fatal Chromobacterium violaceum septicaemia in northern Laos, a modified oxidase test and post-mortem forensic family G6PD analysis (2009)
  • Author(s): Slesak G, Douangdala P, Inthalad S, Silisouk J, Vongsouvath M, Senguangphachanh A, Moore CE, Mayxay M, Matsuoka H, Newton PN
  • Journal Title: Annals Clin Microbiol Antimicrobials 8 Pages: 24-24
  • Peer Reviewed
• [Journal Article] G6PD deficiency assessment in Freetown, Sierra Leone, reveals further insight into the molecular heterogeneity of G6PD A-. (2008)
  • Author(s): Jalloh A, Jalloh M, Gamanga I, Baion D, Sahr F, Gbakima A, Willoughby VR, Matsuoka H
  • Journal Title: J Hum Genet 53(7) Pages: 675-679
  • NAID: 10021249964
• [Journal Article] Nine different glucose-6-phosphate dehydrogenase (G6PD) variants in a Malaysian population with Malay, Chinese, Indian and Orang Asli (Aboriginal Malaysian) backgrounds. (2008)
  • Author(s): Wang J, Luo E, Hirai M, Arai M, Abdul-Manan EAS, Mohamed-Isa Z, Hidayah NI, Matsuoka H
  • Journal Title: Acta Med Okayama 62(5) Pages: 327-332
  • NAID: 120002305207
• [Journal Article] Nine different glucose-6-phosphate dehydrogenase (G6PD)variants in a Malaysian population with Malay, Chinese, Indian and Orang Asli (Aboriginal Malaysian)backgrounds (2008)
  • Author(s): Wang J, Luo E, Hirai M, Arai M, Abdul-Manan EAS, Mohamed-Isa Z, Hidayah NI, Matsuoka H
  • Journal Title: Acta Medica Okayama 53 Pages: 327-332
  • NAID: 120002305207
  • Peer Reviewed
• [Journal Article] G6PD deficiency assessment in Freetown, Sierra Leone, reveals further insight into the molecular heterogeneity of G6PD A- (2008)
  • Author(s): Jalloh A, Jalloh M, Gamanga I, Gbakima FSA, Willoughby VR, Matsuoka H
  • Journal Title: Journal of Human Genetics 53 Pages: 675-679
  • NAID: 10021249964
  • Peer Reviewed
• [Presentation] 三日熱マラリアおよび卵型マラリアの根治療法に先立つG6PD活性測定の意義 (2010)
  • Author(s): 松岡裕之
  • Organizer: 第70回日本寄生虫学会東日本支部会(抄録集p17)
  • Place of Presentation: 栃木県壬生町
  • Year and Date: 2010-10-02
• [Presentation] Importance of glucose-6-phosphate dehydrogenase (G6PD) activity test prior to the treatment of vivax malaria and ovale malaria. (2010)
  • Author(s): Matsuoka H, Kawamoto F
  • Organizer: 第51回日本熱帯医学会大会(抄録集p63)
  • Place of Presentation: 仙台市
• [Presentation] Importance of glucose-6-phosphate dehydrogenase (G6PD) activity test priorto the treatment of vivax malaria and ovale malaria (2010)
  • Author(s): Matsuoka H
  • Organizer: 第51回日本熱帯医学会大会
  • Place of Presentation: 東北大学(仙台)
• [Presentation] アジア・アフリカにおけるG6PD変異症および日本国内におけるG6PD変異症の状況について (2008)
  • Author(s): 松岡裕之, 平井誠, Amadu Jalloh
  • Organizer: 第7回分子寄生虫・マラリア研究フォーラム(抄録集p20)
  • Place of Presentation: 松山市
• [Presentation] Introduction of rapid methods for malaria diagnosis and glucose-6-phosphate dehydrogenase activity test in malaria endemic areas in Asian and African countries. (2008)
  • Author(s): Matsuoka H, Jalloh A, Hirai M, Kawamoto F
  • Organizer: XVIIth International Congress for Tropical Medicine and Malaria.(Abstract p422)
  • Place of Presentation: Korea
• [Presentation] Field surveys for malaria and glucose-6-phosphate dehydrogenase deficiency in eastern Indonesia (2008)
  • Author(s): Kawamoto F, Tantular IS, Kasahara Y, Matsuoka H, Kanbe T
  • Organizer: 第77回日本寄生虫学会大会(抄録集p72)
  • Place of Presentation: 長崎市
• [Presentation] Introduction of rapid methods for malaria diagnosis and glucose-6-phosphate dehydrogenase activity test in malaria endemic areas in Asian and African countries. (2008)
  • Author(s): Matsuoka H, Jalloh A, Hirai M, Kawamoto F
  • Organizer: XVIIth International Congress for TropicalMedicine and Malaria
  • Place of Presentation: 韓国 済州島
• [Presentation] アジア・アフリカにおけるG6PD変異症および日本国内におけるG6PD変異症の状況について (2008)
  • Author(s): 松岡裕之, 平井誠, Amadu Jalloh
  • Organizer: 第7回分子寄生虫・マラリア研究フォーラム
  • Place of Presentation: 愛媛県 松山市
• [Remarks] ホームページ等
  • URL: http://www.jichi.ac.jp/idoubutsu/MedZool-home.html