| Project/Area Number |
20406023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
MATSUOKA Hiroyuki Jichi Medical University, 医学部, 教授 (10173816)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Makoto 群馬大学, 医学部, 講師 (50326849)
佐野 元市郎 自治医科大学, 医学部, 助教 (30383730)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMOTO Fumihiko 大分大学, 医学部, 教授 (40115556)
MATSUDA Hajime 獨協医科大学, 医学部, 名誉教授 (30114648)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | グルコース6リン酸脱水素酵素 / 先天性代謝異常症 / 溶血発作マラリア / プリマキン / 感染蚊対策 / 分子疫学 / グルコニス6リン酸脱水素酵素 / マラリア / 人類遺伝学 / 溶血性貧血 / 新生児黄疸 / 東南アジア / シエラレオネ |
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| Research Abstract |
We visited malaria endemic areas in Asian and African countries to introduce rapid methods for malaria diagnosis and glucose-6-phosphate dehydrogenase (G6PD) activity test. For malaria diagnosis, acridine orange method was employed. For G6PD activity test, WST-8 method was used. These methods were done at the site of malaria endemic areas and results were informed within 30 minutes after taking blood. Patients could receive anti-malarial medicine accordingly including primaquine to eliminate gametocytes. Primaquine was not used for the patients with G6PD deficiency. WST-8 method was useful for the detection of G6PD deficiency in rural areas without electricity and was recommendable for use in malaria control programs. We found more than 700 G6PD deficient people in these activities. DNA analysis of G6PD gene was performed after receiving informed consent. Thus we read about 400 samples and found 22 molecular variants including 5 new G6PD variants.
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