Analysis on the present state in multi-drug resistant Mycobacterium tuberculosis infection and its clinical background in China

• Project/Area Number: 20406024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Infectious disease medicine
• Research Institution: Tohoku University
• Principal Investigator: KAWAKAMI Kazuyoshi 東北大学, 大学院・医学系研究科, 教授 (10253973)
• Co-Investigator(Renkei-kenkyūsha): KAKU Koki 防衛大学医学校, 准教授 (40431499) ; YAMAMOTO Natsuo 福島県立医科大学, 検査部, 講師 (50466562)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: アジア / 結核 / 耐性菌

Research Abstract

We investigated the present state in multi-drug resistant tuberculosis (TB)in Guangzhou City, Guangdong Province, China by collaborating with the Third Affiliated Hospital of Sun Yat-Sen University and Guangzhou Chest Hospital. In the Chest Hospital, the proportion of MDR-TB and extensively drug-resistant (XDR)-TB was 16. 7%and 2. 0%in 2008, 9. 1%and 0. 9%in 2009 and 13. 5%and 0. 3%in 2010, respectively, which showed that XDR-TB tended to be decreased during the study period though no change in MDR-TB. We sequenced the drug resistance genes, rpoB, kat G, inhA and ahpC, in 50 strains of MDR-TB and 5 strains of XDR-TB, and mutation was detected at 90%, 63%, 22%and 6%, respectively. In addition, we analyzed the host factors in these patients by measuring BMI, serum albumin levels, WBC counts, proportion of T and NK cells, CD4/CD8 ratio and reactivity of peripheral blood lymphocytes to TB antigens, and we did not find any significant difference between drug-sensitive and MDR-TB.