Epidemiological and virological studies on avian influenza virus H5N1 in Egypt

• Project/Area Number: 20406025
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Infectious disease medicine
• Research Institution: Osaka University
• Principal Investigator: IKUTA Kazuyoshi Osaka University, 微生物病研究所, 教授 (60127181)
• Co-Investigator(Kenkyū-buntansha): SAKUDO Akikazu 琉球大学, 医学部・保健学科, 助教 (10397672) ; NAKAYA Takaaki 大阪大学, 微生物病研究所, 准教授 (80271633) ; TAKAHASHI Kazuo 大阪府立公衆衛生研究所, 副所長 (10171472) ; 纐纈 律子 財団法人阪大微生物病研究会, 共同研究員 (80467521)
• Co-Investigator(Renkei-kenkyūsha): OKUNO Yoshinobu (財)阪大微生物病研究会, 観音寺研究所, 所長 (30112064) ; KOKETSU-KUBOTA Ritsuko (財)阪大微生物病研究会, 研究員 (80467521) ; WATANABE Yohei 大阪大学, 微生物病研究所, 特任研究員 (50452462) ; SASAKI TADAHIRO 大阪大学, 微生物病研究所, 特任研究員 (20547533)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 鳥インフルエンザウイルス / エジプト / アレキサンドリア大学 / ニワトリ / アヒル / ハト / 遺伝子配列 / 病原性

Research Abstract

Highly pathogenic avian influenza A virus (HPAIV H5N1) is currently widespread in several areas. Although the current H1N1 pandemic may have diverted attention from the continuing worldwide circulation of H5N1 virus, the pandemic threat of H5N1 is still alarming. In particular, Egypt has unexpectedly had the highest number of human cases of H5N1 infection. In this study, we conducted a molecular-epidemiological studies using H5N1 viruses isolated between 2006 and 2008-2009 in Egypt. The phylogenetic results showed that recent human isolates clustered into several new H5 sublineages. As expected, we found that these H5 sublineages have acquired an enhanced binding affinity for α2, 6SA.

Research Products

• [Journal Article] Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1 Clin. (2011)
  • Author(s): Mizuike R, Sasaki T, Baba K, Iwamoto H, Shibai Y, Kosaka M, Kubota-Koketsu R, Yang C-S, Du A, Sakudo A, Tsujikawa M, Yunoki M, Ikuta K
  • Journal Title: Vaccine Immunol. 18 Pages: 494-499
  • Peer Reviewed
• [Journal Article] Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1. (2011)
  • Author(s): Mizuike, R., Sasaki, T., Baba, K., Iwamoto, H., Shibai, Y., Kosaka, M., Kubota-Koketsu, R., Yang, C.-S., Du, A., Sakudo, A., Tsujikawa, M., Yunoki, M., Ikuta, K.
  • Journal Title: Clin.Vaccine Immunol. Volume: 18 Pages: 494-499
  • Peer Reviewed
• [Journal Article] Host-specific genetic variation of highly pathogenic avian influenza viruses (H5N1). (2011)
  • Author(s): Ibrahim, M.S.Watanabae, Y., Ellakany, H.F., Yamagishi, A., Sapsutthipas, S., Toyoda, T., Abd El-Hamied, H.S., Ikuta, K.
  • Journal Title: Virus Genes Volume: (印刷中 In press)
  • Peer Reviewed
• [Journal Article] Sawanpanyalert P.Distinct propagation efficiencies of H5N1 influenza virus Thai isolates in newly established murine respiratory region-derived cell clones. (2010)
  • Author(s): Kanai Y, Chittaganpitch M, Nakamura I, Li GM, Bai GR, Li YG, Ikuta K
  • Journal Title: Virus Res. 153 Pages: 218-225
  • Peer Reviewed
• [Journal Article] Ramasoota P.Fab MAbs specific to HA of influenza virus with H5N1 neutralizing activity selected from immunized chicken phage library. (2010)
  • Author(s): Pitaksajjakul P, Lekcharoensuk P, Uparagarin N, Barbas CF, 3^<rd>, Ibrahim MS, Ikuta K
  • Journal Title: Biochem.Biophys.Res.Commun. 395 Pages: 496-501
  • Peer Reviewed
• [Journal Article] Sawanpanyalert P.Characterization of H5N1 influenza viruses isolated from humans in vitro. (2010)
  • Author(s): Li YG, Chittaganpitch M, Waicharoen S, Kanai Y, Bai GR, Kameoka M, Takeda N, Ikuta K
  • Journal Title: Virological J. 7 Pages: 112-112
  • Peer Reviewed
• [Journal Article] Highly conserved sequences for human neutralization epitope on hema.gglutinin of influenza A viruses H3N2, H1N1 and H5N1 : Implication for humanmonoclonal antibody recognition. (2010)
  • Author(s): Yamashita A, Kawashita N, Kubota-Koketsu R, Inoue Y, Watanabe Y, Ibrahim MS, Ideno S, Yunoki M, Okuno Y, Takagi T, Yasunaga T, Ikuta K
  • Journal Title: Biochem.Biophys.Res.Commun. 393 Pages: 614-618
  • Peer Reviewed
• [Journal Article] Highly pathogenic H5N1 avian influenza virus induces extracellular Ca2+ influx, leading to apoptosis in avian cells. (2010)
  • Author(s): Ueda M, Daidoji T, Du A, Yang CS, Ibrahim MS, Ikuta K, Nakaya T
  • Journal Title: J.Virol. 84 Pages: 3068-3078
  • Peer Reviewed
• [Journal Article] Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. (2010)
  • Author(s): Kayamuro, H., Yoshioka, Y., Abe, Y., Arita, S., Katayama, K., Nomura, T., Yoshikawa, T., Kubota-Koketsu, R., Ikuta, K., Okamoto, S., Mori, Y., Kunisawa, J., Kiyono, H., Itoh, N., Nagano, K., Kamada, H., Tsutsumi, Y., Tsunoda, S.
  • Journal Title: J.Virol. Volume: 84 Pages: 12703-12712
  • Peer Reviewed
• [Journal Article] Distinct propagation efficiencies of H5N1 influenza virus Thai isolates in newly established murine respiratory region-derived cell clones. (2010)
  • Author(s): Kanai, Y., Chittaganpitch, M., Nakamura, I., Li, G.M., Bai, G.R., Li, Y.G., Ikuta, K., Sawanpanyalert, P.
  • Journal Title: Virus Res. Volume: 153 Pages: 218-225
  • Peer Reviewed
• [Journal Article] Fab MAbs specific to HA of influenza virus with H5N1 neutralizing activity selected from immunized chicken phage library. (2010)
  • Author(s): Pitaksajjakul, P., Lekcharoensuk, P., Uparagarin, N., Barbas, C.F., 3^<rd>., Ibrahim, M.S., Ikuta, K., Ramasoota, P.
  • Journal Title: Biochem.Biopys.Res.Commun. Volume: 395 Pages: 496-501
  • Peer Reviewed
• [Journal Article] The impact of antigenic drift of influenza A virus on human herdimmunity : Sero-epidemiological study of H1N1 in healthy Thai population in 2009. (2010)
  • Author(s): Kanai, Y., Boonsathorn, N., Chittaganpitch, M., Bai, G., Li, Y., Kase, T., Takahashi, K., Okuno, Y., Jampangern, W., Ikuta, K., Sawanpanyalert, P.
  • Journal Title: Vaccine Volume: 28 Pages: 5437-5444
  • Peer Reviewed
• [Journal Article] Characterization of H5N1 influenza viruses isolated from humans in vitro. (2010)
  • Author(s): Li, Y.G., Chittaganpitch, M., Waicharoen, S., Kanai, Y., Bai, G.R., Kameoka, M., Takeda, N., Ikuta, K., Sawanpanyalert, P.
  • Journal Title: Virological J. Volume: 7 Pages: 112-112
  • Peer Reviewed
• [Journal Article] Highly conserved sequences for human neutralization epitope on hemagglutinin of influenza A viruses H3N2, H1N1 and H5N1 : Implication for human monoclonal antibody recognition. (2010)
  • Author(s): Yamashita, A., Kawashita, N., Kubota-Koketsu, R., Inoue, Y., Watanabe, Y., Ibrahim, M.S., Ideno, S., Yunoki, M., Okuno, Y., Takagi, T., Yasunaga, T., Ikuta, K.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 393 Pages: 614-618
  • Peer Reviewed
• [Journal Article] Highly pathogenic H5N1 avian influenza virus induces extracellular Ca2+ influx, leading to apoptosis in avian cells. (2010)
  • Author(s): Ueda, M., Daidoji, T., Du, A., Yang, C.S., Ibrahim, M.S., Ikuta, K., Nakaya, T.
  • Journal Title: J.Virol. Volume: 84 Pages: 3068-3078
  • Peer Reviewed
• [Journal Article] Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1. (2010)
  • Author(s): Yunoki, M., Kubota-Koketsu, R., Urayama, T., Sasaki, T., Du, A., Konoshima, Y., Ideno, S., Fukunaga, Y., Morikawa, S., Hiroi, S., Takahashi, K., Okuno, Y., Hagiwara, K., Ikuta, K.
  • Journal Title: Br.J.Haematol. Volume: 148 Pages: 953-955
  • Peer Reviewed
• [Journal Article] Highly pathogenic H5N1 avian influenza virus induces extracellular Ca2+ influx, leading to apoptosis in avian cells. (2010)
  • Author(s): Ueda M, Daidoji T, Du A, Yang CS, Ibrahim MS, Ikuta K, Nakaya T.: Ueda M, Daidoji T, Du A, Yang CS, Ibrahim M S, Ikuta K, Nakaya T.
  • Journal Title: J.Virol. 84 Pages: 3068-3078
  • Peer Reviewed
• [Journal Article] Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach. (2009)
  • Author(s): Nakamura S, Yang CS, Sakon N, Ueda M, Tougan T, Yamashita A, Goto N, Takahashi K, Yasunaga T, Ikuta K, Mizutani T, Okamoto Y, Tagami M, Morita R, Maeda N, Kawai J, Hayashizaki Y, Nagai Y, Horii T, Iida T, Nakaya T
  • Journal Title: PLoS One 4
  • Peer Reviewed
• [Journal Article] Anionic polymer (2009)
  • Author(s): Sakudo A, Baba K, Tsukamoto M, Sugimoto A, Okada T, Kobayashi T, Kawashita N, Takagi T, Ikuta K
  • Journal Title: poly(methyl vinyl ether-maleic anhydride)-coated beads-based capture of human influenza A and B virus. Bioorg.Med.Chem. 17 Pages: 752-757
  • Peer Reviewed
• [Journal Article] Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody. (2009)
  • Author(s): Du A, Daidoji T, Koma T, Ibrahim MS, Nakamura S, de Silva UC, Ueda M, Yang CS, Yasunaga T, Ikuta K, Nakaya T
  • Journal Title: Biochem.Biophys.Res.Commun. 378 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus. (2009)
  • Author(s): Ueda M, Yamate M, Du A, Daidoji T, Okuno Y, Ikuta K, Nakaya T
  • Journal Title: Virus.Res. 136 Pages: 91-97
  • Peer Reviewed
• [Journal Article] Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody. (2009)
  • Author(s): Du, A., Daidoji, T., Koma, T., Ibrahim, M.S., Nakamura, S., de Silva, U.C., Ueda, M., Yang, C.S., Yasunaga, T., Ikuta, K., Nakaya, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 378 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Anionic polymer, poly(methyl vinyl ether-maleic anhydride)-coated beads-based capture of human influenza A and B virus. (2009)
  • Author(s): Sakudo, A., Baba, K., Tsukamo to, M., Sugimoto, A., Okada, T., Kobayashi, T., Kawashita, N., Takagi, T., Ikuta, K.
  • Journal Title: Bioorg. Med. Chem. 17 Pages: 752-757
  • Peer Reviewed
• [Journal Article] Direct metagenomic detection of viral pathogens in nasal and fecal specimens using an unbiased high-throughput sequencing approach. (2009)
  • Author(s): Nakamura, S., Yang, C.S., Sakon, N., Ueda, M., Tougan, T., Yamashita, A., Goto, N., Takahashi, K., Yasunaga, T., Ikuta, K., Mizutani, T., Okamoto, Y., Tagami, M., Morita, R., Maeda, N., Kawai, J., Hayashizaki, Y., Nagai, Y., Horii, T., Iida, T., Nakaya, T.
  • Journal Title: PLoS One 4
  • Peer Reviewed
• [Journal Article] Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors. (2009)
  • Author(s): Kubota-Koketsu, R., Mizuta, H., Oshita, M., Ideno, S., Yunoki, M., Kuhara, M., Yamamoto, N., Okuno, Y., Ikuta, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 387 Pages: 180-185
  • Peer Reviewed
• [Journal Article] Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand (2009)
  • Author(s): Bai, G.-R., Chittaganpitch, M., Kawai, Y., Li, Y.- G., Auwanit, W., Ikuta, K., Sawanpanyalert, P.
  • Journal Title: Biochem. Biophys. Res. Commun. 390 Pages: 897-901
  • Peer Reviewed
• [Journal Article] Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1. (2009)
  • Author(s): Yunoki, M., Kubota-Koketsu, R., Urayama, T., Sasaki, T., Du, A., Konoshima, Y., Ideno, S., Fukunaga, Y., Morikawa, S., Hiroi, S., Takahashi, K., Okuno, Y., Hagiwara, K, Ikuta, K.
  • Journal Title: Br. J.Haematol. 143 Pages: 953-955
  • Peer Reviewed
• [Journal Article] Highly conserved sequences for human neutralization epitope on hemagglutinin of influenza A viruses H3N2, H1N1 and H5N1 : Implication for human monoclonal antibody recognition. (2009)
  • Author(s): Yamashita, A., Kawashita, N., Kubota-Koketsu, R., Inoue, Y., Watanabe, Y., Ibrahim, M.S., Ideno, S., Yunoki, M., Okuno, Y., Takagi, T., Yasunaga, T., Ikuta, K.
  • Journal Title: Biochem. Biophys. Res. Commun. In press
  • Peer Reviewed
• [Journal Article] Detection of cshed monoclonarculating Asian H5N1 viruses by a newly establiantibody. (2009)
  • Author(s): Du A., et al.
  • Journal Title: Biochem Biophys Res Commun. 378 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Efficient capture of infectious H5 avian influenza virus utilizing magnetic beads coated with anionic polymer. (2008)
  • Author(s): Sakudo A, Ikuta K
  • Journal Title: Biochem.Biophys.Res.Commun. 377 Pages: 85-88
  • Peer Reviewed
• [Journal Article] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells. (2008)
  • Author(s): Daidoji T, Koma T, Du A, Yang CS, Ueda M, Ikuta K, Nakaya T
  • Journal Title: J.Virol. 82 Pages: 11294-11307
  • Peer Reviewed
• [Journal Article] Flu pandemic : the host or the hosting land? (2008)
  • Author(s): Ikuta K., et al.
  • Journal Title: Future Virology 3 Pages: 197-201
  • Peer Reviewed
• [Journal Article] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells. (2008)
  • Author(s): Daidoji T., et al.
  • Journal Title: J Virol. 82 Pages: 11294-11307
  • Peer Reviewed
• [Journal Article] Efficient capture of infectious H5 avzing magnetic beads coated with an ionan influenza virus utilic polymer. (2008)
  • Author(s): Sakudo A., et al.
  • Journal Title: Biochem Biophys Res Commun. 377 Pages: 85-88
  • Peer Reviewed
• [Journal Article] Acquisition of Human-Type Receptor Binding Specificity by New H5N1 Influenza Virus Sublineages during Their Emergence in Birds in Egypt.
  • Author(s): Watanabe Y, Ibrahim MS, Ellakany HF, Kawashita N, Mizuike R, Hiramatsu H, Sriwilaijaroen N, Takagi T, Suzuki Y, Ikuta K
  • Journal Title: PLoS Pathog. in press
  • Peer Reviewed
• [Journal Article] Host-specific genetic variation of highly pathogenic avian influenza viruses (H5N1).
  • Author(s): Ibrahim MS, Watanabe Y, Ellakany HF, Yamagishi A, Sapsutthipas S, Toyoda T, Abd El-Hamied HS, Ikuta K
  • Journal Title: Virus Genes in press
  • Peer Reviewed
• [Presentation] エジプトで流行するH5N1高病原性鳥インフルエンザウイルスのヒト型レセプター結合親和性獲得に関与する変異 (2010)
  • Author(s): 渡辺洋平、M.S.Ibrahim、川下理日人、H.F.Ellakany、N.Scriwilaijaroen、平松宏明、鈴木康夫、生田和良
  • Organizer: ウイルス学会
  • Place of Presentation: 徳島市 あわぎんホール
  • Year and Date: 2010-11-08
• [Presentation] Evolutionary Dynamics of Highly Pathogenic Avian Influenza Virus H5N1 in Egypt (2010)
  • Author(s): Watanabe Y, Ibrahim MS, Ellakany HF, Abd El-Hamid HS, Ikuta K
  • Organizer: 2nd Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2010-04-17
• [Presentation] Development of Diagnostic and Therapeutic Products of Murine and Human Monoclonal Antibodies against Influenza Virus (2010)
  • Author(s): 生田和良
  • Organizer: 3rd International Conference of Virology, Egyptian Society of Virology
  • Place of Presentation: Cairo, Egypt
• [Presentation] エジプトで流行するH5N1高病原性鳥インフルエンザウイルスのヒト型レセプター結合親和性獲得に関与する変異 (2010)
  • Author(s): 渡邊洋平、Madiha S. Ibrahim, 川下理日人、Hany F. Ellakany, Nongluk Sriwilaijaroen, 平松宏明、鈴木康夫、生田和良
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] アヒル固体内の各臓器で選択されたH5N1高病原性鳥インフルエンザウイルスの性状 (2009)
  • Author(s): 渡辺洋平、M.S.Ibrahim, H.F.Ellakany, 生田和良
  • Organizer: ウイルス学会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2009-09-26
• [Presentation] Propagation-specific sequence changes of highly pathogenic avian influenza H5N1viruses (2009)
  • Author(s): Ibrahim, B.S., 渡辺洋平、生田和良
  • Organizer: ウイルス学会
  • Place of Presentation: 都市センターホテル
  • Year and Date: 2009-09-25
• [Presentation] アヒル固体内臓器で選択されたH5N1高病原性鳥インフルエンザウイルスの性状 (2009)
  • Author(s): 渡邊洋平、Madiha S. Ibrahim, Hany F. Ellakany, 生田和良
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
• [Presentation] Propagation-Specific Sequence Changes of Highly Pathogenic Avian Influenza H5N1 Viruses (2009)
  • Author(s): Madiha S. Ibrahim, 渡邊洋平、生田和良
  • Organizer: The 57th Annual Meeting of The Japanese Society for Virology
  • Place of Presentation: 東京
• [Presentation] Preparation of influenza virus-specific human monoclonal antibodies showing broad neutralization activity (2008)
  • Author(s): 生田和良
  • Organizer: Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2008-11-12
• [Presentation] Development of Pre-pandemic Vaccines for Pandemic Influenza in Japan (2008)
  • Author(s): Okuno Y
  • Organizer: Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2008-11-12
• [Presentation] Development of rapid diagnosis test for avian influenza viruses (2008)
  • Author(s): Takahashi K
  • Organizer: Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2008-11-12
• [Presentation] H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells (2008)
  • Author(s): Nakaya T
  • Organizer: Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2008-11-12
• [Presentation] Molecular Characterization of Highly Pathogenic Avian Influenza Viruses from Egypt (2008)
  • Author(s): Ibrahim MS
  • Organizer: Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2008-11-12
• [Presentation] Intra-species shifts of H5N1 Highly Pathogenic Influenza Viruses (2008)
  • Author(s): 渡邊洋平, Ibrahim MS, Ellakany HF, Abd El-Hamid HS, 生田和良
  • Organizer: Joint Scientific Forum on Avian Influenza
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2008-11-12
• [Presentation] Molecular Characterization of highly Pathogenic Avian Influenza Viruses (H5N1) from Egypt (2008)
  • Author(s): Madiha S. Ibrahim, Hany F. Ellakany、渡邊洋平、生田和良
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] H5N1鳥インフルエンザに感染した家禽固体内におけるウイルス遺伝子変異の解析 (2008)
  • Author(s): 渡邊洋平、Madiha S. Ibrahim, Hany F. Ellakany, 生田和良
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] Molecular characterization of highly pathogenic avian influenza virus (H5N1) from Egypt (2008)
  • Author(s): Madiha S. Ibrahim
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] H5N1鳥インフルエンザウイルスに感染した家禽個体内におけるウイルス遺伝子変異の解析 (2008)
  • Author(s): 渡邊 洋平
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Remarks] ホームページ等
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• [Remarks]
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• [Remarks]
  • URL: http://virology.biken.osaka-u.ac.jp/index.html