Research of the scaffold protein JSAP1 during the differentiation of cerebellar granule cell precursors

• Project/Area Number: 20500282
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Kanazawa University
• Principal Investigator: YOSHIOKA Katsuji Kanazawa University, がん研究所, 教授 (60200937)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マウス / 小脳 / 顆粒前駆細胞 / シグナル伝達 / 足場タンパク質 / 発生・分化 / MAPキナーゼ / JNK / 顆粒細胞

Research Abstract

We performed immunohistochemical analysis and found that JSAP1, a scaffold protein for JNK signaling pathways, is expressed predominantly in the post-mitotic granule cell precursors (GCPs) of the inner external granular layer. We also showed that when stimulated by FGF receptor, JSAP1 translocates to the plasma membrane, where it recruits JNK and facilitates the activation of JNK, leading to the differentiation of cerebellar GCPs. Furthermore, we found that JSAP1-JNK signaling promotes the cell cycle exit and differentiation of cerebellar GCPs.

Research Products

• [Journal Article] N-cadherin regulates p38 MAPK signaling via association with JNK-associated leucine zipper protein : implications for neurodegeneration in Alzheimer disease. (2011)
  • Author(s): Ando, K., Uemura, K., Kuzuya, A., Maesako, M., Asada-Utsugi, M., Kubota, M., Aoyagi, N.Yoshioka, K., Okawa, K., Inoue, H., Kawamata, J., Shimohama, S., Arai, T., Takahashi, R., Kinoshita, A.
  • Journal Title: J.Biol.Chem. 286 Pages: 7619-7628
  • Peer Reviewed
• [Journal Article] Role of plasma membrane localization of the scaffold protein JSAPI during differentiation of cerebellar granule cell precursors. (2011)
  • Author(s): Sato, T., Enkhbat, A., Yoshioka, K.
  • Journal Title: Genes Cells 16 Pages: 58-68
  • Peer Reviewed
• [Journal Article] N-cadherin regulates p38 MAPK signaling via association with JNK-associated leucine zipper protein : implications for neurodegeneration in Alzheimer disease (2011)
  • Author(s): Ando.K
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 7619-7628
  • Peer Reviewed
• [Journal Article] Role of plasma membrane localization of the scaffold protein JSAP1 during differentiation of cerebellar granule cell precursors (2011)
  • Author(s): Sato.T
  • Journal Title: Genes to Cells Volume: 16 Pages: 58-68
  • Peer Reviewed
• [Journal Article] MafB protein stability is regulated by the JNK and ubiquitin-proteasome pathways. (2010)
  • Author(s): Tanahashi, H., Kito, K., Tto, T., Yoshioka, K.
  • Journal Title: Arch.Biochem.Biophys. 494 Pages: 94-100
  • NAID: 120001828354
  • Peer Reviewed
• [Journal Article] Pathogenic huntingtin inhibits fast axonal transport by activating JNK3 and phosphorylating kinesin. (2009)
  • Author(s): Morfini, G.A., You, Y.M., Pollema, S.L., Kaminska, A., Liu, K., Yoshioka, K., Bjorkblom, B., Coffey, E.T., Bagnato, C., Han, D., Huang, C.E., Banker, G., Pigino, G., Brady, S.T.
  • Journal Title: Nat.Neurosci. 12 Pages: 864-871
  • Peer Reviewed
• [Journal Article] Stress-activated mitogen kinase c-Jun NH_2 terminal kinase and p38 target Cdc25B for degradation. (2009)
  • Author(s): Uchida, S., Yoshioka, K., Kizu, R., Nakagama, H., Matsunaga, T., Ishizaka, Y., Poon, R.Y., Yamashita, K.
  • Journal Title: Cancer Res. 69 Pages: 6438-6444
  • Peer Reviewed
• [Journal Article] JNK-binding protein 1 regulates NF-kappaB activation through TRAF2 and TAKI. (2009)
  • Author(s): Yamaguchi, T., Miyashita, C., Koyano, S., Kanda, H., Yoshioka, K. , Shiba, T., Takamatsu, N., Ito, M.
  • Journal Title: Cell Biol.Int. 33 Pages: 364-368
  • Peer Reviewed
• [Journal Article] Stress-activated mitogen kinase c-Jun NH_2-terminal kinase and p38 target Cdc25B for degradation (2009)
  • Author(s): Uchida.S
  • Journal Title: Cancer Research 69 Pages: 6438-6444
  • Peer Reviewed
• [Journal Article] Pathogenic huntingtin inhibits fast axonal transport by activating JNK3 and phosphorylating kinesin (2009)
  • Author(s): Morfini.G.A.
  • Journal Title: Nature Neuroscience 12 Pages: 864-871
  • Peer Reviewed
• [Journal Article] JNK-binding protein 1 regulates NF-kappaB activation through TRAF2 and TAK1 (2009)
  • Author(s): Yamaguchi.T
  • Journal Title: Cell Biology International 33 Pages: 364-368
  • Peer Reviewed
• [Journal Article] The scaffold protein c-Jun NH2-terminal kinase-associated leucine zipper protein regulates cell migration through interaction with the G protein G(alpha 13). (2008)
  • Author(s): Gantulga, D., Tuvshintugs, B., Endo, Y., Taki no, T., Sato, H., Murakami, S., Yoshioka, K.
  • Journal Title: J.Biochem. 144 Pages: 693-700
  • Peer Reviewed
• [Journal Article] The scaffold protein JSAP1 regulates proliferation and differentiation of cerebellar granule cell precursors by modulating JNK signaling. (2008)
  • Author(s): Sato, T., Torashima, T., Sugihara, K., Hirai, H., Asano, M., Yoshioka, K.
  • Journal Title: Mol.Cell Neurosci. 39 Pages: 569-578
  • NAID: 120000807435
  • Peer Reviewed
• [Journal Article] Ablation of the scaffold protein JLP causes reduced fertility in male mice. (2008)
  • Author(s): Iwanaga, A., Wang, G., Sato, T., Baljinnyam, T., Shimizu, K., Takumi, K., Hayashi, M., Fuse, H., Sugihara, K., Asano, M., Yoshioka, K.
  • Journal Title: Transgenic Res. 17 Pages: 1045-1058
  • NAID: 120000997202
  • Peer Reviewed
• [Journal Article] RNA interference silencing of DRATL affects processing of amyloid precursor protein. (2008)
  • Author(s): Tanahashi, H., Yoshioka K.
  • Journal Title: Neurosci Lett. 439 Pages: 293-297
  • Peer Reviewed
• [Journal Article] The scaffold protein c-Jun NH_2-terminal kinase-associated leucine zipper protein regulates cell migration through interaction with the G protein G_<α13> (2008)
  • Author(s): Davaakhuu Gantulga, Katsuji Yoshioka, 他5名
  • Journal Title: Journal of Biochemistry 144 Pages: 693-700
  • NAID: 10024913756
  • Peer Reviewed
• [Journal Article] The scaffold protein JSAP1 regulates proliferation and differentiation of cerebellar granule cell precursors by modulating JNK signaling (2008)
  • Author(s): Tokiharu Sato, Katsuji Yoshioka, 他4名
  • Journal Title: Molecular and Cellular Neuroscience 39 Pages: 569-578
  • NAID: 120000807435
  • Peer Reviewed
• [Journal Article] Ablation of the scaffold protein JLP causes reduced fertility in malemice (2008)
  • Author(s): Asuka Iwanaga, Katsuji Yoshioka, 他9名
  • Journal Title: Transgenic Research 17 Pages: 1045-1058
  • Peer Reviewed
• [Presentation] (小脳顯粒前駆細胞の増殖・分化過程における足場タンパク質JSAP1の細胞内局在変化)第33回日本分子生物学会年会 (2010)
  • Author(s): 佐藤時春, Enkhbat, A., Baljinnyan, T., 善岡克次
  • Organizer: 第83回日本生化学大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] 小脳顆粒前駆細胞の増殖・分化過程における足場タンパク質JSAP1の細胞内局在変化 (2010)
  • Author(s): 佐藤時春
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] (Subcellular localization of the scaffold protein JSAP1 during the differentiation of cerebellar granule cell precursors)小脳穎粒前駆細胞の分化過程における足場タンパク質JSAP1の細胞内局在 (2010)
  • Author(s): 佐藤時春, Enkhbat,A., 善岡克次
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: リーガロイヤルホテル大阪(大阪府)
  • Year and Date: 2010-09-22
• [Presentation] Subcellular localization of the scaffold protein JSAP1 during the diffrentiation of cerebellar granule cell precursors (2010)
  • Author(s): 佐藤時春
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: リーガロイヤルホテル大阪(大阪府)
  • Year and Date: 2010-09-22
• [Presentation] Role of the scaffold protein JSAP1 during the differentiation of cerebellar granule cell precursors (2010)
  • Author(s): 佐藤時春, 善岡克次.
  • Organizer: The Joint Symposium of the 5th International Symposium of Institute Network and the International Symposium Commemorating Inauguration of Kanazawa University Cancer Research Institute
  • Place of Presentation: KKR Hotel Kanazawa (Ishikawa, Japan).
  • Year and Date: 2010-06-24
• [Presentation] Positive feedback regulation of the scaffold protein Jsapl gene expression during the differentiation of cerebellar granule cell precursors (2009)
  • Author(s): 佐藤時春、Anir Enkhbat、善岡克次
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] Positive feedback regulation of the scaffold protein Jsap1 gene expression during the differentiation of cerebellar granule cell precursors (2009)
  • Author(s): 佐藤時春
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] JNK誘発SCF^<beta-TrCP>依存的Cdc25Bユビキチン化の分子機構 (2009)
  • Author(s): 内田早苗、渡辺信元、工藤保誠、善岡克次、松永司、中釜斉、山下克美
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-10-03
• [Presentation] JSAP-1JNKシグナル伝達系による穎粒前駆細胞の増殖・分化制御 (2008)
  • Author(s): 佐藤時春、寅嶋崇、杉原一司、平井宏和、浅野雅秀、善岡克次
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2008-12-10
• [Presentation] JSAP1-JNKシグナル伝達系による顆粒前駆細胞の増殖・分化制御 (2008)
  • Author(s): 佐藤時春, 善岡克次
  • Organizer: 第31回日本分子生物大会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Remarks] ホームページ等
  • URL: http://www.kanazawa-u.ac.jp/~ganken/department/mccb/03.html
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• [Remarks]
  • URL: http://www.kanazawa-u.ac.jp/~ganken/index.html
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• [Patent(Industrial Property Rights)] 情動障害の治療薬のスクリーニング方法 (2009)
  • Inventor(s): 善岡克次、吉原亨、佐藤時春、浅野雅秀
  • Industrial Property Rights Holder: 国立大学法人金沢大学
  • Industrial Property Number: 2009-220591
  • Filing Date: 2009-09-25