Analys is of exocytotic mechanism of BDNF from astrocytes.

• Project/Area Number: 20500291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Kitasato University
• Principal Investigator: ITAKURA Makoto Kitasato University, 医学部, 講師 (30398581)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: グリア細胞 / 開口放出 / 脳神経栄養因子 / プロテインキナーゼC / SNAP-23 / BDNF / SNAREタンパク質 / siRNA / リン酸化 / リン酸化酵素 / SNARE / matureBDNF / proBDNF

Research Abstract

The brain are made up of many cells, including neurons and glial cells. Astrocytes, a type of glial cells release various bioactive substances via Ca(2+)-and SNARE-dependent exocytosis. In the present study, we revealed that potent protein kinase C activator, phorbol 12-myristate 13-acetate (PMA) treatment suppressed exogenously expressed human growth hormone release from cultured astrocytes. Moreover, PMA treatment induced the phosphorylation of synaptosomal-associated protein of 23 kDa on Ser(95), Ser(120), and Ser(160).

Research Products

• [Journal Article] M. PKC-dependent inhibition of CA2+-dependent exocytosis from astrocytes (2011)
  • Author(s): Yasuda, K., Itakura, M., Aoyagi, K., Sugaya, T., Nagata, E., Ihara, H., Takahashi
  • Journal Title: Glia 59 Pages: 143-151
  • Peer Reviewed
• [Journal Article] PKC-dependent inhibition of Ca2+-dependent exocytosis from astrocytes. (2011)
  • Author(s): Yasuda, K., Itakura, M., et al
  • Journal Title: Glia Volume: 59 Pages: 143-151
  • Peer Reviewed
• [Presentation] M. Phorbol 12-myristate 13-acetate suppresses Cat+-dependent exocytotic release from rat astroglial cells (2009)
  • Author(s): Yasuda, K., Itakura, M., Aoyagi, K., Sugaya, T., Yamamori, S., Takahashi
  • Organizer: 第36回国際生理学会世界大会
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] M. Identification of PKC-dependent phosphorylation sites of SNAP-23inclonalglia cells (2009)
  • Author(s): Yasuda, K., Itakura, M., Nagata, E., Yamamori, S., Ihara, H., Takahashi
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-23
• [Presentation] Identification of PKC-dependent phosphorylation sites of SNAP-23 in clonal glia cells (2009)
  • Author(s): K Yasuda, M ITakura, E Nagata, S Yamamori, H Ihara, M Takahashi
  • Organizer: 第52回 日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-23