Regulation mechanism of inhibitory synaptic plasticity
| Project/Area Number |
20500296
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
KURIU Toshihiko Tokushima Bunri University, 香川薬学部, 助教 (10401374)
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| Co-Investigator(Renkei-kenkyūsha) |
KONISHI Shiro 徳島文理大学, 香川薬学部, 教授 (20014277)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 抑制性シナプス / シナプス可塑性 / シナプス形成 / 神経科学 / 可塑性 / 長期増強 / GABA / 抑制シナプス |
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| Research Abstract |
To visualize dynamics of both presynaptic and postsynaptic structures on inhibitory synapse, we used two distinct pre- and postsynaptic fluorescent probes. Venus-expressing presynaptic inhibitory varicosities markedly change their morphology and the postsynaptic clusters of mCherry-gephyrin dynamically move around inhibitory synaptic sites. These movements of inhibitory synaptic contact sites stabilized depending on the length of culture period. The motilities of preaynaptic varicosities and postsynaptic gephyrin clusters significantly decreased during the period of the maximum increases in their densities. They underwent characteristic long-lasting changes (fusion, fission, movement) in their shape during synaptogenesis. These results suggest that motilities of pre- and postsynaptic structure could play an important role in the inhibitory synapse formation, including rearrangements during synaptogenesis.
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Report
(4 results)
Research Products
(13 results)
