Physiological functions of proteolipid family proteins

• Project/Area Number: 20500298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Genetics
• Principal Investigator: HIRATA Tatsumi National Institute of Genetics, 総合遺伝研究系, 准教授 (80260587)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経 / 発生 / 軸索伸長 / ノックアウトマウス / ノツクアウトマウス / proteolipid蛋白質

Research Abstract

The proteolipid family is a group of four transmembrane proteins that are highly expressed in the nervous systems. To gain a better understanding of physiological functions of these proteins, we analyzed brain phenotypes of gene knockout mice for M6a and M6b that belong to this family. Although no significant abnormalities have been so far detected in each single mutant, we found hypoplasia of the corpus callosum in the double mutant mice that had homozygous mutations in both of the genes. Primary culture analyses suggested that the neurons of these mutant mice have impaired ability to extend neurites.

Research Products

• [Journal Article] Proteomics Analysis of the Temporal Changes in Axonal Proteins during Maturation. (2010)
  • Author(s): Yamatani H., Kawasaki T., Mita.S., Inagaki N., Hirata T.
  • Journal Title: Dev.Neurobiol. 70 Pages: 523-537
  • Peer Reviewed
• [Journal Article] Proteomics Analysis of the Temporal Changes in Axonal Proteins during Maturation (2010)
  • Author(s): Yamatani, et al.
  • Journal Title: Dev.Neurobiol. Volume: 70 Pages: 523-537
  • Peer Reviewed
• [Journal Article] Proteomics Analysis of the Temporal Changes in Axonal Proteins during Maturation (2010)
  • Author(s): Yamatani, et al.
  • Journal Title: Dev.Neurobiol. (印刷中)
  • Peer Reviewed
• [Journal Article] The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells. (2009)
  • Author(s): Nakadate N., Uchida K., Shikata K., Yoshimura, S., Azuma M., Hirata T., Konishi H., Kiyama H., Tachibana T.
  • Journal Title: Biochem.Biophys.Res.Comm. 378 Pages: 209-212
  • Peer Reviewed
• [Journal Article] The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells. (2009)
  • Author(s): Nakadate Y, et al.
  • Journal Title: Biochem. Biophys. Res. Comm. 37 Pages: 209-212
  • Peer Reviewed
• [Journal Article] Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface. (2008)
  • Author(s): Ito K., Kawasaki T., Takashima S., Matsuda I., Aiba A., Hirata, T.
  • Journal Title: J.Neurosci. 28 Pages: 4414-4422
  • Peer Reviewed
• [Journal Article] Involvement of stem cell factor and its receptor tyrosine kinase c-kit in pain regulation. (2008)
  • Author(s): Takagi K., Okuda-Ashitaka E., Mabuchi T., Katano T., Ohnishi T., Matsumura S., Abe T., Hirata, T., Minami T., Ito S.
  • Journal Title: Neuroscience 153 Pages: 1278-1288
  • Peer Reviewed
• [Journal Article] Identification of presomitic mesoderm (PSM)-specific Mesp1 enhancer and generation of a PSM-specific Mesp1/Mesp2-null mouse using BAC-based rescue technology. (2008)
  • Author(s): Oginuma M., Hirata T., Saga Y.
  • Journal Title: Mech.Dev. 125 Pages: 432-440
  • Peer Reviewed
• [Journal Article] Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface. (2008)
  • Author(s): Ito K, et al.
  • Journal Title: J. Neurosci 28 Pages: 4414-4422
  • Peer Reviewed
• [Journal Article] Involvement of stem cell factor and its receptor tyrosine kinase c-kit in pain regulation. (2008)
  • Author(s): Takagi K, at al.
  • Journal Title: Neuroscience 153 Pages: 1278-1288
  • Peer Reviewed
• [Journal Article] Induction of axon growth arrest without growth cone collapse through the N-terminal region of four-transmembrane glycoprotein M6a.
  • Author(s): Sato Y., Mita S., Fukushima N., Fujisawa H., Saga, Y., Hirata,, T.
  • Journal Title: Dev.Neurobiol. (in press)
  • Peer Reviewed
• [Presentation] M6 proteins regulate axon outgrowth in mouse callosal neurons. (2010)
  • Author(s): Mita S., Saga Y., Werner H.B., Nave K-A, Hirata T.
  • Organizer: 第33回日本神経科学会年会
  • Place of Presentation: 神戸
• [Presentation] M6 proteins regulate axon outgrowth in mouse callosal neurons (2010)
  • Author(s): Mita, et al.
  • Organizer: 第33回日本神経科学会年会
  • Place of Presentation: 神戸
• [Presentation] Axon outgrowth inhibition mediated by four-transmembrane protein M6a. (2008)
  • Author(s): Hirata, T.
  • Organizer: San Fransisco-Japan Joint Meeting on Vertebrate Organogenesis
  • Place of Presentation: San Francisco, USA.
  • Year and Date: 2008-11-24
• [Presentation] Axon outgrowth inhibition mediated by four-transmembrane protein M6a. (2008)
  • Author(s): Hirata T.
  • Organizer: UTMD Anderson Cancer Center/RIKEN-CDB Joint Symposium : Vertebrate Development and Organogenesis
  • Place of Presentation: Houston, USA.
  • Year and Date: 2008-11-21
• [Presentation] Axon outgrowth inhibition mediated by a growth cone membrane protein. (2008)
  • Author(s): Hirata T.
  • Organizer: IISER-SOKENDAI Lecture Workshop on "Trends in Modern Biology"
  • Place of Presentation: Pune, India.
  • Year and Date: 2008-10-24
• [Presentation] Axon outgrowth inhibition mediated by a growth cone membrane protein. (2008)
  • Author(s): Hirata T.
  • Organizer: Scientific Colloquium at Department of Zoology, University of Delhi
  • Place of Presentation: Delhi, India.
  • Year and Date: 2008-10-22
• [Remarks] ホームページ等
  • URL: http://www.nig.ac.jp/section/hirata/hirata-j.html