Molecular mechanisms for regional specification in the retina

• Project/Area Number: 20500299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute for Basic Biology
• Principal Investigator: SAKUTA Hiraki National Institute for Basic Biology, 統合神経生物学研究部門, 助教 (40343743)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 網膜視蓋投射 / CBF2 / 領域特異化 / 網膜 / ニワトリ

Research Abstract

Misexpression of CBF2 represses the expression of CBF1, GH6, SOHo1, and ephrin-A5, and induces that of EphA3 in the retina. GH6 and SOHo1 repress the expression of CBF2. In contrast to the inhibitory effect of CBF1 on bone morphogenic protein (BMP) signaling, CBF2 does not alter the expression of BMP4 or BMP2. Studies with chimeric mutants of CBF2 showed that CBF2 acts as a transcription repressor in controlling its downstream targets in the retina. Furthermore, Fgf and Wnt first play pivotal roles in inducing the region-specific expression of CBF1 and CBF2 in the optic vesicle.

Research Products

• [Journal Article] Functional mode of FoxD1/CBF2 for the establishment of temporal retinal specificity in the developing chick retina. (2009)
  • Author(s): Takahashi H., Sakuta H., Shintani T., Noda M.
  • Journal Title: Dev Biol 331 Pages: 300-310
  • Peer Reviewed
• [Journal Article] Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion. (2009)
  • Author(s): Yonehara K., Ishikane H., Sakuta H., Shintani T., Nakamura-Yonehara K., Kamiji N. L., Usui S., Noda M.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Functional mode of FoxD1/CBF2 for the establishment of temporal retinal specificity in the developing chick retina (2009)
  • Author(s): Takahashi, H., et al.
  • Journal Title: Dev.Biol. 331 Pages: 300-310
  • Peer Reviewed
• [Journal Article] APC2 plays an essential role in axonal projections through the regulation of microtubule stability (2009)
  • Author(s): Shintani, T., et al.
  • Journal Title: J.Neurosci. 29 Pages: 11628-11640
  • Peer Reviewed
• [Journal Article] Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Sakuta, H.
  • Journal Title: Springer Pages: 105-116
• [Journal Article] Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion (2009)
  • Author(s): Yonehara, K.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation. (2008)
  • Author(s): Sakuta H., Suzuki R., Noda M.
  • Journal Title: Dev Growth Differ 50 Pages: 453-457
• [Journal Article] Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation (2008)
  • Author(s): Sakuta, H.
  • Journal Title: Dev. Growth Differ. 50 Pages: 453-457
• [Presentation] SPIG1の発現により明らかになったON中心型方向選択性神経節細胞サブタイプの発達様式 (2008)
  • Author(s): 米原圭祐、新谷隆史、鈴木亮子、作田拓、竹内靖、中村-米原佳世、石金浩史、Nilton L.Kamiji、臼井支朗、野田昌晴
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Book] Electroporation and Sonoporation in Developmental Biology (H. Nakamura, ed.)(Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation.) (2009)
  • Author(s): Sakuta H., Suzuki R., Noda M.
  • Publisher: Springer, Springer-Verlag
• [Book] Encyclopedia of Neuroscience 4th ed.(L. M. Squire et al., eds.)(Neural Patterning : Eye fields.) (2009)
  • Author(s): Noda M., Takahashi H., Sakuta H.
  • Publisher: Elsevier B. V., Amsterdam
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