Analysis of synaptic molecules trafficking by using 2-photon activation of photo-activatable molecule

• Project/Area Number: 20500304
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KIYOSUE Kazuyuki National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 研究グループ付 (50356903)
• Co-Investigator(Kenkyū-buntansha): KAMEYAMA Kimihiko 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50224697)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 神経科学 / 脳・神経科学 / 光スイッチ / 可視化 / 脳・神経 / 2光子吸収

Research Abstract

Memory formation at synapse is a kind of transformation from electrical activity to morphological and composing protein changes Although synaptic molecules are localized at synapse by passive and positive transportation and assembled by themselves autonomically, it is unclear whether the behavior of synaptic molecules are changed by synaptic activation. We examined paGFP-tagged CaMKII behavior at synapse by 2p-photoconversion. The motility of CaMKII were slower than that of GluR1 receptors. This indicates that CaMKII which has been activated once can activate AMPA receptors coming into the synapse.

Research Products

• [Journal Article] 受容体の膜輸送によるシナプス機能制御 (2010)
  • Author(s): 清末和之
  • Journal Title: 生体の科学特集シナプスをめぐるシグナリング Vol61, No5 Pages: 534-536
• [Journal Article] 受容体の膜輸送によるシナプス機能制御 (2010)
  • Author(s): 清末和之
  • Journal Title: 生体の科学 特集 シナプスをめぐるシグナリング Volume: Vol61,No5 Pages: 534-536
• [Presentation] Tracking of synaptic proteins at synapse by photoactivatable GFP in hippocampal neurons (2009)
  • Author(s): 清末和之
  • Organizer: 日本生物物理学会
  • Place of Presentation: アスティ徳島(徳島県)
  • Year and Date: 2009-10-30