| Project/Area Number |
20500316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
IIJIMA Norio (2009-2010) Nippon Medical School, 医学部, 准教授 (00285248)
薛 昊がん (2008) Nippon Medical School, 医学部, 助教 (10328809)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Masakatsu 日本医科大学, 医学部, 准教授 (90143239)
戸張 靖子 日本医科大学, 大学院・医学研究科, ポストドクター (90453919)
飯島 典生 日本医科大学, 医学部, 准教授 (00285248)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 視床下部 / 生殖神経内分泌 / GnRH / 免疫組織化学 / kisspeptin / Dopamine, / プロラクチン / GnRHニューロン / kisspeptinニューロン / TIDAニューロン / ラット / 思春期 / spine / 視索前野 / 形態変化 / 生殖 |
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| Research Abstract |
We made a specific polyclonal antibody against kisspeptin which is a remarkable regulatory peptide for hypothalamo-pituitary-gonadal (HPG) axis. Immunohistochemistry using this antibody revealed that a few kisspeptin-immunoreactive (ir) fibers were located in GnRH cell bodies and did not show any direct contact between them. However, kisspeptin-ir fibers were densely distributed in the dorsal part of arcuate nucleus in which dopamine neurons were located, which were called TIDA neurons. The morphological investigation in this study revealed that kisspeptin-ir fibers showed direct contact with TIDA neurons. In addition, physiological experiments revealed that TIDA neurons showed a increase of intracellular calcium and expression of cFos in response to kisspeptin. Thus, we conclude that kisspeptin regulates the prolactin secretion from the pituitary via the TIDA neurons. Furthermore, this knowledge suggests that kisspeptin regulates the HPG axis via prolactin which permeate into the brain.
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