Visualization of strategic localization of synaptic molecules

• Project/Area Number: 20500317
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: KAMASAWA Naomi National Institute for Physiological Sciences, 大脳皮質機能研究系, 特任助教 (50455218)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 脳・神経 / シナプス / 受容体 / チャネル / 電子顕微鏡 / レプリカ標識法 / グルタミン酸受容体 / カルシウムチャネル / 可視化 / 凍結割断レプリカ免疫コロイド金標識法

Research Abstract

Synaptic membrane molecules, glutamate receptors and calcium channels were ultrastructurally visualized in the calyx of Held synapse using freeze-fracture replica immunogold labeling methods. Presynaptic and postsynaptic components were synchronously localized to the synapses at the timing of the onset of hearing.

Research Products

• [Journal Article] Immunolocalization of multiple membrane proteins on a carbon replica with X STEM and EDX (2010)
  • Author(s): Loukanov A, Kamasawa N, Danev R, Shigemoto R, Nagayama K
  • Journal Title: Ultramicroscopy 110巻 Pages: 366-374
  • Peer Reviewed
• [Journal Article] Immunolocalization of multiple membrane proteins on a carbon replica with STEM and EDX (2010)
  • Author(s): Loukanov A, Kamasawa N, Danev R, Shigemoto R, Nagayama K
  • Journal Title: Ultramicroscopy 110 Pages: 366-374
  • Peer Reviewed
• [Journal Article] Connexin45-Containing Neuronal Gap Junctions in Rodent Retina Also Contain Connexin36 in Both Apposing Hemiplaques, Forming Bihomotypic Gap Junctions, with Scaffolding Contributed by Zonula Occludens-1 (2008)
  • Author(s): Li, N Kamasawa, C Ciolofan, CO Olson, S Lu, KGV Davidson, T Yasumura, R Shigemoto, JE Rash, JI Nagy
  • Journal Title: The Journal of Neuroscience 28巻 Pages: 9769-9789
  • Peer Reviewed
• [Journal Article] Connexin45-Containing Neuronal Gap Junctions in Rodent Retina Also Contain Connexin36 in Both Apposing Hemiplaques, Forming Bihomotypic Gap Junctions, with Scaffolding Contributed by Zonula Occludens-1 (2008)
  • Author(s): X Li, N Kamasawa, C Ciolofan, CO Olson, S Lu, KGV Davidson, T Yasumura, R Shigemoto, JE Rash, JI Nagy
  • Journal Title: The Journal of Neuroscience 28 Pages: 9769-9789
  • Peer Reviewed
• [Presentation] 生体分子の局在分布から機能を考える (2010)
  • Author(s): 釜澤尚美
  • Organizer: 日本顕微鏡学会 生体構造解析分科会 2010年度研究討論会
  • Place of Presentation: 湯沢ニューオータニホテル(新潟県)
  • Year and Date: 2010-12-27
• [Presentation] 生体分子の局在分布から機能を考える (2010)
  • Author(s): 釜澤尚美
  • Organizer: 日本顕微鏡学会生体構造解析分科会2010年度研究討論会
  • Place of Presentation: 揚沢ニューオータニホテル(新潟県)
  • Year and Date: 2010-12-27
• [Presentation] Ryuichi Shigemoto 「Quantitative ocalization of α1A subunit of P/A type calcium channel in rat cerebellum as revealed by SDS-Freeze Fracture replica labeling」 (2010)
  • Author(s): Dwi Wahyu Indriati, Naomi Kamasawa, Masahiko Watanabe
  • Organizer: 7th FENS (Forum of European Neuroscience)
  • Place of Presentation: アムステルダム(オランダ)
  • Year and Date: 2010-07-07
• [Presentation] Quantitative ocalization ofα1A subunit of P/A type calcium channel in rat cerebellum as revealed by SDS-Freeze Fracture replica labeling (2010)
  • Author(s): Dwi Wahyu Indriati, Naomi Kamasawa, Masahiko Watanabe, Ryuichi Shigemoto
  • Organizer: 7^<th> FENS(Forum of European Neuroscience)
  • Place of Presentation: アムステルダム(オランダ)
  • Year and Date: 2010-07-07
• [Presentation] 凍結割断レプリカ標識法によるカルシウムチャネルとグルタミン酸受容体の局在解析 (2010)
  • Author(s): 釜澤尚美、渡辺雅彦、重本隆一
  • Organizer: 日本顕微鏡学会第65回学術講演会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2010-05-25
• [Presentation] Ryuichi ShigemotoDevelopmental clustering of glutamate receptors in the Calyx of Held synapses (2009)
  • Author(s): Naomi Kamasawa, Ko Matsui, Timotheus Budisantoso
  • Organizer: Neuroscience 2009
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Developmental clustering of glutamate receptors in the Calyx of Held synapses (2009)
  • Author(s): Naomi Kamasawa, Ko Matsui, Timotheus Budisantoso, Ryuichi Shigemoto
  • Organizer: Neuroscience 2009
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Development and function of glutamate receptor clustering in the Calyx of Held synapses. (2009)
  • Author(s): Naomi Kamasawa, Ko Matsui, Timotheus Budisantoso, Ryuichi Shigemoto
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: 京都国際センター(京都府)
  • Year and Date: 2009-07-28
• [Presentation] Development and function of glutamate receptor clustering in the Calyx of Held synapses (2009)
  • Author(s): Naomi Kamasawa, Ko Matsui, Timotheus Budisantoso, Ryuichi Shigemoto
  • Organizer: The 36^<th> Congress of the International Union of Physiological Sciences
  • Place of Presentation: 京都国際センター(京都府)
  • Year and Date: 2009-07-28
• [Presentation] 双面レプリカ標識法を用いたシナプスの観察 (2009)
  • Author(s): 釜澤尚美、重本隆一
  • Organizer: 日本顕微鏡学会第65回学術講演会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2009-05-27
• [Presentation] 凍結割断レプリカ標識法で観察した台形体核グルタミン酸作動性シナプスの発達過程 (2009)
  • Author(s): 釜澤尚美、重本隆一
  • Organizer: 日本顕微鏡学会第65回学術講演会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2009-05-27
• [Presentation] ラット嗅鞘細胞における電位依存性Naチャネルの免疫局在 (2009)
  • Author(s): 長谷川瑠美、高見茂、釜澤尚美、重本隆一
  • Organizer: 日本顕微鏡学会第65回学術講演会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2009-05-27
• [Presentation] Double-replica labeling reveals that connexin-45 is accompanied by connexin-36 adult rodent retina, forming bi-homotypic gap junctions. (2008)
  • Author(s): Naomi Kamasawa, X Li, KGV. Davidson, T Yasumura, JI NAGY, R SHIGEMOTO, JE RASH
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-19
• [Presentation] Double-replica labeling reveals that connexin-45 is accompanied by connexin-36 adult rodent retina, forming bi-homotypic gap junctions (2008)
  • Author(s): N KAMASAWA, X LI, KGV. DAVIDSON, T YASUMURA, JI NAGY, R SHIGEMOTO, JE RASH
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-19
• [Presentation] 台形体核におけるグルタミン酸作動生シナプスの凍結割断レプリカ標識法による解析 (2008)
  • Author(s): 釜澤尚美、重本隆一
  • Organizer: 日本顕微鏡学会第64回学術講演会
  • Place of Presentation: 京都国際センター(京都府)
  • Year and Date: 2008-05-22
• [Presentation] 台形体核におけるグルタミン酸作動生シナプスの凍結割断レプリカ標識法による解析 (2008)
  • Author(s): 釜澤尚美、重本隆一
  • Organizer: 日本顕微鏡学会第64回学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2008-05-22